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Purified anti-Musashi-1 Antibody

Pricing & Availability
Clone
3F8-E6-B9 (See other available formats)
Regulatory Status
RUO
Other Names
RNA-binding protein Musashi homolog 1, Musashi-1, MSI1, Musashi RNA Binding Protein 1
Isotype
Mouse IgG2b, κ
Ave. Rating
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Product Citations
publications
3F8-E6-B9_PURE_Musashi-1_Antibody_1_073119
Western blot of purified anti-Musashi-1 antibody (clone 3F8-E6-B9). Lane 1: Molecular weight marker; Lane 2: 20 μg of normal human brain lysate; Lane 3: 20 μg of Alzheimer's disease brain lysate. The blot was incubated with 0.5 μg/mL of the primary antibody overnight at 4°C, followed by incubation with HRP-labeled goat anti-mouse IgG (Cat. No. 405306). Enhanced chemiluminescence was used as the detection system.
  • 3F8-E6-B9_PURE_Musashi-1_Antibody_1_073119
    Western blot of purified anti-Musashi-1 antibody (clone 3F8-E6-B9). Lane 1: Molecular weight marker; Lane 2: 20 μg of normal human brain lysate; Lane 3: 20 μg of Alzheimer's disease brain lysate. The blot was incubated with 0.5 μg/mL of the primary antibody overnight at 4°C, followed by incubation with HRP-labeled goat anti-mouse IgG (Cat. No. 405306). Enhanced chemiluminescence was used as the detection system.
  • 3F8-E6-B9_PURE_Musashi-1_Antibody_2_073119
    Western blot of purified anti-Musashi-1 antibody (clone 3F8-E6-B9). Lane 1: Molecular weight marker; Lane 2: 20 μg of rat brain lysate; Lane 3: 20 μg of mouse brain lysate. The blot was incubated with 0.5 μg/mL of anti-Musashi-1 (left panel) or anti-mouse IgG2b (right panel, isotype control) antibodies, followed by incubation with HRP-labeled goat anti-mouse IgG (Cat. No. 405306). Enhanced chemiluminescence was used as the detection system.
  • 3F8-E6-B9_PURE_Musashi-1_Antibody_3_073119
    IHC staining of purified anti-Musashi-1 antibody (clone 3F8-E6- B9) on formalin-fixed paraffin-embedded mouse brain tissue. Following antigen retrieval using Sodium Citrate H.I.E.R., the tissue was incubated with 0.5 µg/mL of the primary antibody overnight at 4°C. BioLegend's Ultra Streptavidin (USA) HRP Detection Kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale bar: 50 µm
  • 3F8-E6-B9_PURE_Musashi-1_Antibody_4_073119
    IHC staining of purified anti-Musashi-1 antibody (clone 3F8-E6- B9) on formalin-fixed paraffin-embedded mouse brain tissue. Following antigen retrieval using Sodium Citrate H.I.E.R., the tissue was incubated with 0.5 µg/mL of the primary antibody overnight at 4°C. BioLegend's Ultra Streptavidin (USA) HRP Detection Kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale bar: 50 µm
  • 3F8-E6-B9_PURE_Musashi-1_Antibody_5_073119
    IHC staining of purified anti-Musashi-1 antibody (clone 3F8-E6- B9) on formalin-fixed paraffin-embedded rat brain tissue. Following antigen retrieval using Sodium Citrate H.I.E.R., the tissue was incubated with 0.5 µg/mL of the primary antibody overnight at 4°C. BioLegend's Ultra Streptavidin (USA) HRP Detection Kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale bar: 50 µm
  • 3F8-E6-B9_PURE_Musashi-1_Antibody_6_073119
    IHC staining of purified anti-Musashi-1 antibody (clone 3F8-E6- B9) on formalin-fixed paraffin-embedded rat brain tissue. Following antigen retrieval using Sodium Citrate H.I.E.R., the tissue was incubated with 0.5 µg/mL of the primary antibody overnight at 4°C. BioLegend's Ultra Streptavidin (USA) HRP Detection Kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale bar: 50 µm
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869101 25 µg 85€
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869102 100 µg 221€
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Description

Musashi-1 (MSI1) is an evolutionarily conserved RNA-binding protein with essential role in stem cell maintenance, nervous system development, and tumorigenesis. It was first identified in Drosophila as a protein required for the development of adult external sensory organs (sensilla) and neuronal potential in asymmetric cell division. In mammalian nervous systems, MSI1 is one of the key oncogenic factors for glioblastomas and medulloblastomas. The expression of MSI1 is associated with Notch signaling. The high expression of MSI1 is a marker of poor prognosis in medulloblastoma. Genomic analysis indicates that MSI1 functions as a master regulator of genes during development and tumor formation. 

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human, Mouse, Rat
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Human Musashi-1 recombinant protein
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
IHC-P - Verified
Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.5 - 10 µg per ml. For immunohistochemistry, a concentration range of 0.5 - 10 µg/ml is suggested. It is recommended that the reagent be titrated for optimal performance for each application.

Product Citations
  1. Chen L, et al. 2021. J Biol Chem. 297:101275. PubMed
RRID
AB_2814629 (BioLegend Cat. No. 869101)
AB_2814629 (BioLegend Cat. No. 869102)

Antigen Details

Structure
Human MSI1 is a 362 amino acid protein with a predicted and observed molecular mass of 39 kD.
Distribution

Tissue distribution: Central nervous system, fetal kidney, liver, lung, and pancreas

Cellular distribution: Nucleus and cytoplasm

Function
MSI1 is an RNA binding protein that regulates the expression of target mRNAs at the translation level.
Cell Type
Neural Stem Cells, Neurons, Oligodendrocytes
Biology Area
Cancer Biomarkers, Neurodegeneration, Neuroscience
Molecular Family
Enzymes and Regulators
Antigen References
  1. Okabe M, et al. 2001. Nature. 411:94-8. 
  2. de Sousa Abreu R. et al. 2009. J Biol Chem. 284(18):12125-35.
  3. Vo DT. et al. 2012. Am J Pathol. 181(5):1762-72. 
  4. Sengupta U. et al. 2018. Acta Neuropathol Commun. 6(1):113.
Gene ID
4440 View all products for this Gene ID
UniProt
View information about Musashi-1 on UniProt.org
Go To Top Version: 1    Revision Date: 07.31.2019

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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