- Clone
- CTD110.6 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- O-Linked N-Acetylglucosamine
- Isotype
- Mouse IgM, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
Cat # | Size | Price | Quantity Check Availability | Save | ||
---|---|---|---|---|---|---|
838004 | 100 µg | 278€ |
β-O-linked N-acetylglucosamine (O-GlcNAc) is an abundant post-translational modification occuring on serine and threonine residues. The modification has been detected on a variety of nuclear and cytoplasmic proteins involved in diverse cellular functions. Studies to elucidate the role of O-GlcNAc-ylation have been hampered by the lack of a sensitive, specific assay to identify or purify proteins bearing O-GlcNAc.
Product DetailsProduct Details
- Verified Reactivity
- All Species
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- The antibody was raised against a synthetic peptide containing Serine-O-GlcNAc.
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography.
- Concentration
- 0.5 mg/mL
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C.
- Application
-
WB - Quality tested
ICC, IHC-P - Verified
IP - Reported in the literature, not verified in house - Recommended Usage
-
Each lot of this antibody is quality control tested by western blotting. For immunocytochemistry, a concentration range of 2.5 - 10 μg/mL is recommended. For immunohistochemical staining on formalin-fixed paraffin-embedded tissue sections, the suggested use of this reagent is 1.0 - 10 µg/mL. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
-
This antibody detects Ser-O-GlcNAc and Thr-O-GlcNAc but shows no cross-reactivity with peptide determinants or other closely-related carbohydrate antigens. Interaction between the antibody and the Ser- or Thr-O-GlcNAc epitope can be blocked by competition with free GlcNAc. Milk proteins may cross-react with the antibody therefore we recommend using BSA or goat serum as a blocker.
Additional reported applications (for the relevant formats) include: ELISA2, immunocytochemistry7, immunoprecipitation2,5,6, and Western blotting3,4,7. -
Application References
(PubMed link indicates BioLegend citation) -
- Comer FI, et al. 2001. Anal. Biochem. 293:169.
- Hart GW, et al. 1995. Adv. Exp. Med. Biol. 376:115. (ELISA, WB, IP)
- Anthonisen EH, et al. 2010. J. Biol. Chem. 285:1607. (WB)
- Dias WB, et al. 2009. J. Biol. Chem. 32:21327. (WB)
- Wang Z, et al. 2007. Mol. Cell Proteomics 8:1365. (IP)
- Kim SJ, et al. 2003. J. Biol. Chem. 43:41890. (IP)
- Kneass ZT, Marchase RB. 2004. J. Biol. Chem. 44:45759. (WB, ICC, IF)
- Product Citations
-
- RRID
-
AB_2629520 (BioLegend Cat. No. 838004)
Antigen Details
- Structure
- 1046 amino acids with a molecular weight of 117 kD.
- Distribution
-
Cytosol, mitochondrion, nucleus, plasma membrane, and cytoskeleton.
- Function
- Catalyzes the transfer of a single N-acetylglucosamine from UDP-GlcNAc to a serine or threonine residue in cytoplasmic and nuclear proteins resulting in their modification with a beta-linked N-acetylglucosamine (O-GlcNAc), Glycosylates a large and diverse number of proteins including histone H2B, AKT1, EZH2, PFKL, KMT2E/MLL5, MAPT/TAU and HCFC1, Can regulate their cellular processes via cross-talk between glycosylation and phosphorylation or by affecting proteolytic processing.
- Biology Area
- Cell Biology, Transcription Factors
- Gene ID
- 8473 View all products for this Gene ID
- UniProt
- View information about O-GlcNAc on UniProt.org
Related Pages & Pathways
Pages
Other Formats
View All O-GlcNAc Reagents Request Custom ConjugationDescription | Clone | Applications |
---|---|---|
Purified anti-O-GlcNAc | CTD110.6 | WB,ICC,IHC-P,IP |
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