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Purified anti-p38 MAPK Antibody

Pricing & Availability
Clone
Poly6224 (See other available formats)
Regulatory Status
RUO
Other Names
Stress activated kinase p38, p38 MAP kinase, stress activated protein kinase 2A
Isotype
Rabbit Polyclonal IgG
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Product Citations
publications
Poly6224_PURE_p38_MAPK_Antibody_1_072120.png
Whole cell extracts (15 µg protein) from NTERA-2, HeLa, Jurkat and NIH/3T3 cells were resolved by electrophoresis (4-12% Bis-Tris), transferred to PVDF, and probed overnight with a 1:4000 dilution of purified anti-p38 MAPK antibody (clone Poly6224). Proteins were visualized by chemiluminescent detection using HRP donkey anti-rabbit IgG antibody (Cat. No. 406401). Direct-Blot™ HRP anti-β-actin antibody (Cat. No. 664804) was used as a loading control. Lane M: Molecular weight ladder.
  • Poly6224_PURE_p38_MAPK_Antibody_1_072120.png
    Whole cell extracts (15 µg protein) from NTERA-2, HeLa, Jurkat and NIH/3T3 cells were resolved by electrophoresis (4-12% Bis-Tris), transferred to PVDF, and probed overnight with a 1:4000 dilution of purified anti-p38 MAPK antibody (clone Poly6224). Proteins were visualized by chemiluminescent detection using HRP donkey anti-rabbit IgG antibody (Cat. No. 406401). Direct-Blot™ HRP anti-β-actin antibody (Cat. No. 664804) was used as a loading control. Lane M: Molecular weight ladder.
  • Poly6224_PURE_p38_MAPK_Antibody_2_072120.png
    Human paraffin-embedded placenta tissue slices were prepared with a standard protocol of deparaffinization and rehydration. Antigen retrieval was done with 1X Citrate Buffer (diluted from 10X Citrate Buffer, Cat. No. 420902) at 95°C for 40 minutes. Tissue was then stained with a 1:200 dilution of purified anti-p38 MAPK antibody (clone Poly6224) at 4°C overnight, followed by 2.5 µg/mL (1:250 dilution) of Alexa Fluor® 594 donkey anti-rabbit IgG (Cat. No. 406418) antibody for two hours at room temperature. Nuclei were counterstained with DAPI (blue). The image was captured with a 10X objective.
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622403 50 µL 95€
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Description

p38 mitogen-activated protein kinase (MAPK) is a member of the stress activated kinase family with a molecular weight of 38 kD. MAPK can be activated by a number of stimuli including heat, changes in osmolarity, irradiation, and cold. The MAPKs require dual phosphorylation (threonine and tyrosine) for enzymatic activation. The p38 MAP kinase is required for G2 cell cycle delay after UV irradiation, also participates in cytokine production (IL-6 and erythropoietin). The Poly6224 antibody recognizes human, mouse, and rat p38 MAPK and has been shown to be useful for Western blotting.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse, Rat, Human
Antibody Type
Polyclonal
Host Species
Rabbit
Immunogen
peptide-KLH, C terminal
Formulation
PBS containing 0.09% sodium azide and 0.2% gelatin.

Previous lots of this product may have been formulated with 0.1% or 0.05% NaN3 instead of 0.09% NaN3. For further information please contact BioLegend Technical Support or Customer Service.
Preparation
The antibody was purified by affinity chromatography.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
IHC-P - Verified
Recommended Usage

Each lot of this antibody is quality control tested by western blotting. For western blotting, the suggested use of this reagent is 1:4000 dilution. For immunohistochemistry on formalin-fixed paraffin-embedded tissue sections, a dilution of 1:200 is suggested. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

This product may contain other non-IgG subtypes.

Application References

(PubMed link indicates BioLegend citation)
  1. da Silva, et al. 2011. Biochim Biophys Acta. 181:1863. PubMed
  2. Chen W, et al. 2012. Am J Physiol Lung Cell Mol Physiol. 303:279. PubMed
  3. Maeda S, et al. 2014. J Biol Chem. 289:23786. PubMed
Product Citations
  1. Amirova KM, et al. 2023. Int J Mol Sci. 24:. PubMed
  2. Jiang Q, et al. 2022. Stem Cells Int. 2022:5897194. PubMed
RRID
AB_2876740 (BioLegend Cat. No. 622403)

Antigen Details

Structure
Belongs to serine/threonine kinase family, MAP subfamily, 38 kD
Distribution

Ubiquitously expressed, nuclear and cytoplasmic

Function
p38 MAPK plays a critical role in the initiation of G2 delay after ultraviolet radiation; gene knock-out studies have also revealed a critical role for p38 in cardiac remodeling. Downstream targets of p38 include the transcription factors ELK1 and ATF2, MEF2C and MAX.
Interaction
Interacts with MAX, Cdc25B, Cdc25C. Binds to kinase interaction domain in the protein tyrosine phosphatase PTPRR; this interaction retains p38 MAPK in the cytoplasm.
Modification
Phosphorylation
Biology Area
Cell Biology
Molecular Family
Protein Kinases/Phosphatase
Antigen References

1. Bulavin DV, et al. 2001. Nature 411:102.
2. Han J, et al. 1994. Science 265:808.
3. Lee JC, et al. 1994. Nature 372:739.

Regulation
Activated by environmental stress, many pro-inflammatory cytokines and lipopolysaccharide. Dual phosphorylation by MAP2K3 and MAP2K6 is required for activation of p38 MAPK.
Gene ID
80824 View all products for this Gene ID
UniProt
View information about p38 MAPK on UniProt.org
Go To Top Version: 2    Revision Date: 02.08.2024

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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