- Clone
- PY20 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- p-Tyr
- Isotype
- Mouse IgG2b, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
Cat # | Size | Price | Quantity Check Availability | Save | ||
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309301 | 25 µg | 44€ | ||||
309302 | 100 µg | 85€ |
Phosphorylation is a common modification of proteins that can result in alterations in protein function, protein-protein association, cellular localization, and protein-half life. Phosphorylation can occur on threonine, serine, and tyrosine residues. The PY20 monoclonal antibody recognizes phosphorylated tyrosine residues in all species tested (human, mouse, rat, dog, chicken, and frog). The PY20 antibody has been shown to be useful for flow cytometry, immunoprecipitation, Western blotting, and immunofluorescence staining.
Product DetailsProduct Details
- Verified Reactivity
- Human, Mouse, Rat, All Species
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- KLH-conjugated phosphotyrosine
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 50% glycerol. Final antibody concentration is 0.5 mg/ml.
- Preparation
- The antibody was purified by affinity chromatography.
- Concentration
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored at -20°C.
- Application
-
WB - Quality tested
ICC - Verified
ICFC, IP- Reported in the literature, not verified in house - Recommended Usage
-
Each lot of this antibody is quality control tested by Western blotting. Suggested working dilution(s): Use 0.125 - 1.0 µg per ml antibody dilution buffer per mini-gel. Do not use dilution or blocking buffers containing milk as they may interfere with antibody binding to proteins of interest. Dilution and blocking buffers containing 4% bovine serum albumin are recommended for use with this antibody. For immunocytochemistry, a concentration range of 0.2 - 0.5 μg/ml is recommended. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
-
Additional reported applications (for the relevant formats) include: immunoprecipitation1,2, Western blotting1,2, immunocytochemistry3.
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Application References
(PubMed link indicates BioLegend citation) -
- Vuori K, et al. 1995. J. Biol. Chem. 270:22259. (IP, WB)
- Glenney J, et al. 1988. J. Immunol. Meth. 109:277. (IP, WB)
- Prahalad P, et al. 2004. Am J Physiol Cell Physiol 286:C693. (ICC)
- Zentillin L, et al. 2009. FASEB J. 24:1467. PubMed
- Philipsen L, et al. 2013. Mol Cell Proteomics. 12:2551. PubMed
- Cespedes PF, et al. 2014. PNAS. 111:3214. PubMed
- Product Citations
-
- RRID
-
AB_314763 (BioLegend Cat. No. 309301)
AB_314763 (BioLegend Cat. No. 309302)
Antigen Details
- Distribution
-
Phospho-Specific
- Function
- Phosphorylation of specific tyrosine residues, signal transduction, cell cycle progression, oncogenic transformation
- Biology Area
- Cell Biology, Immunology, Signal Transduction
- Molecular Family
- Phospho-Proteins, Protein Kinases/Phosphatase
- Gene ID
- 5800 View all products for this Gene ID
- UniProt
- View information about Phosphotyrosine on UniProt.org
Other Formats
View All Phosphotyrosine Reagents Request Custom ConjugationDescription | Clone | Applications |
---|---|---|
Biotin anti-Phosphotyrosine | PY20 | WB |
Purified anti-Phosphotyrosine | PY20 | WB,ICC,ICFC,IP |
Alexa Fluor® 488 anti-Phosphotyrosine | PY20 | ICFC |
Alexa Fluor® 647 anti-Phosphotyrosine | PY20 | ICFC |
APC anti-Phosphotyrosine | PY20 | ICFC |
PE anti-Phosphotyrosine | PY20 | ICFC |
Alexa Fluor® 594 anti-Phosphotyrosine | PY20 | ICC |
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Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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Biotin anti-Phosphotyrosine
Western blot analysis of Hela untreated (lane 1) cell and He... -
Purified anti-Phosphotyrosine
Total cell lysate (15 µg protein) from non-treated and 1mM P... NIH3T3 cells were non-treated (A and B) or treated with 1mM ... -
Alexa Fluor® 488 anti-Phosphotyrosine
Hydrogen peroxide stimulated EL4 cells intracellularly stain... -
Alexa Fluor® 647 anti-Phosphotyrosine
Hydrogen peroxide stimulated EL4 cells intracellularly stain... -
APC anti-Phosphotyrosine
Human T leukemia cell line Jurkat was cultured in serum-free... -
PE anti-Phosphotyrosine
Human T leukemia cell line Jurkat was cultured in serum-free... -
Alexa Fluor® 594 anti-Phosphotyrosine
NIH3T3 cells were non-treated (A-C) or treated with 1mM perv...
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