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Purified anti-PTK2B Antibody

Pricing & Availability
Clone
W20075C (See other available formats)
Regulatory Status
RUO
Other Names
Protein-tyrosine kinase 2-beta, FAK2, CADTK, RAFTK, PYK2, FADK2, CAK-beta, CAKB, Proline-rich tyrosine kinase 2, Related adhesion focal tyrosine kinase
Isotype
Rat IgG2b, κ
Ave. Rating
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Product Citations
publications
A_W20075C_PURE_PTK2B_Antibody_1_10192022
Whole cell extracts (15 µg total protein) from the indicated cell lines were resolved on a 4-12% Bis-Tris gel, transferred to a PVDF membrane, and probed with 1 µg/mL (1:500 dilution) of purified anti-PTK2B (clone W20075C) overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP goat anti-rat IgG (Cat. No. 405405) at a 1:3000 dilution. Direct-Blot™ HRP anti-GAPDH (Cat. No. 607904) was used as a loading control at a 1:50000 dilution. Western-Ready™ ECL substrate premium kit (Cat. No. 426319) was used as a detection agent. Lane M: Molecular weight marker
  • A_W20075C_PURE_PTK2B_Antibody_1_10192022
    Whole cell extracts (15 µg total protein) from the indicated cell lines were resolved on a 4-12% Bis-Tris gel, transferred to a PVDF membrane, and probed with 1 µg/mL (1:500 dilution) of purified anti-PTK2B (clone W20075C) overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP goat anti-rat IgG (Cat. No. 405405) at a 1:3000 dilution. Direct-Blot™ HRP anti-GAPDH (Cat. No. 607904) was used as a loading control at a 1:50000 dilution. Western-Ready™ ECL substrate premium kit (Cat. No. 426319) was used as a detection agent. Lane M: Molecular weight marker
  • B_W20075C_PURE_PTK2B_Antibody_ICC_120122
    ICC staining of purified anti-PTK2B (clone W20075C) on Daudi cells. The cells were fixed and permeabilized with iced-cold 100% methanol, and blocked with 5% FBS for 1 hour at room temperature. The cells were then stained with 5.0 µg/mL of the primary antibody, followed by incubation with 2.5 µg/mL of Alexa Fluor® 594 Goat anti-rat IgG (red) (Cat. No. 405422) for 1 hour at room temperature. Nuclei were counterstained with DAPI (blue) (Cat. No. 422801). Scale bar: 20 μm
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608151 25 µg 95€
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608152 100 µg 235€
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Description

Protein-tyrosine kinase 2-beta (PTK2B or PYK2), a calcium-dependent non-receptor tyrosine kinase, belongs to focal adhesion kinase family and shares approximately 47% amino acid sequence with FAK (or PTK2). As a member of focal adhesion family, PTK2B’s phosphorylation enables interactions with other scaffold and effector proteins that regulate cell migration, adhesion, spreading, actin cytoskeleton reorganization and focal adhesion contact. PTK2B responds to several cellular stimuli to regulate critical phycological functions such as G-protein coupled receptor stimulation in vascular muscle cells, T cell and B cell antigen receptor engagement, and calcium-activated MAP kinase signaling pathway in neuronal cells. A genome-wide research suggests PTK2B correlates with Alzheimer’s disease and is able to reduce the Tau toxicity in animal models.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
E. coli derived recombinant fragment of human PTK2B
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested

ICC - Verified
Recommended Usage

Each lot of this antibody is quality control tested by western blotting. For western blotting, the suggested use of this reagent is 0.1 - 1.0 µg/mL. For immunocytochemistry, a concentration range of 1.25 - 5.0 μg/mL is recommended. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

This product does not cross react with mouse in western blotting. This product is verified to work in immunocytochemistry with methanol only fixed cells but not paraformaldehyde-fixed cells. This product is not suitable for IHC-P (fluorescence).

RRID
AB_2927961 (BioLegend Cat. No. 608151)
AB_2927961 (BioLegend Cat. No. 608152)

Antigen Details

Structure
A 1009 amino-acid protein with a predicted molecular weight of 115.8 kD; forms homodimers, or homooligomers
Distribution

Detected in cytosol and membrane; expressed preferentially in endothelium, neuronal and hematopoietic tissue

Function
A protein kinase participates in cytoskeleton rearrangement, cell spreading and cell migration; regulates humoral immune response, angiogenesis and bone remodeling
Interaction
Interacts with paxillin, RHOU, PDPK1, SRC family, and CRK-associated substrate (CAS) family
Cell Type
B cells, Basophils, Dendritic cells, Endothelial cells, Eosinophils, Lymphocytes, Monocytes, Neurons, Neutrophils, NK cells, NKT cells, T cells, Tregs
Biology Area
Cancer Biomarkers, Cell Adhesion, Cell Motility/Cytoskeleton/Structure, Neurodegeneration, Neuroscience, Signal Transduction
Molecular Family
Adaptor Proteins, Adhesion Molecules, APP/Aβ Degradation, Protein Kinases/Phosphatase, Synaptic Vesicle Trafficking/Endocytosis
Antigen References
  1. Lev S, et al. 1995. Nature. 376:737-45.
  2. Astier A, et al. 1997. J Biol Chem. 272:228-32.
  3. Qian D, et al. 1997. J Exp Med. 185:1253-9.
  4. Lambert JC, et al. 2013. Nat Genet. 45:1452-8.
  5. Gurakt A, et al. 2019. Exp Neurol. 307:62-73.
  6. Dourlen P, et al. 2017. Mol Psychiatry. 22:874-883.
Gene ID
2185 View all products for this Gene ID
UniProt
View information about PTK2B on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 10.19.2022

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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