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Purified anti-rat CD8b Antibody

Pricing & Availability
Clone
341 (See other available formats)
Regulatory Status
RUO
Other Names
CD8, Lyt3
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
341_Pure_021208
LOU rat splenocytes stained with purified 341, followed by anti-mouse IgG FITC
  • 341_Pure_021208
    LOU rat splenocytes stained with purified 341, followed by anti-mouse IgG FITC
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200702 500 µg 141€
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Description

CD8b antigen is also known as T8, Lyt2, Ly-2, and CD8β. It is a member of the immunoglobulin superfamily expressed on most thymocytes, some mature T cell subsets, and macrophages. CD8b forms heterodimers with the CD8α chain (CD8a). CD8 is an antigen co-receptor on T cells that interacts with MHC class I on antigen-presenting cells or epithelial cells. CD8 participates in T cell activation through its association with the T cell receptor complex and protein tyrosine kinase lck (p56lck).

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Rat
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
CD8+ Wistar rat splenic T cell hybridomas
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

FC - Quality tested
IP, WB, IHC-F - Reported in the literature, not verified in house
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Additional reported applications (for the relevant formats) include: immunoprecipitation1, Western blotting1, immunohistochemical staining1,2 of acetone-fixed frozen sections and Zinc-fixed paraffin-embeded sections, in vitro blocking of allogeneic responses1, Macrophage stimulation3,4 and in vivo depletion.

Application References

(PubMed link indicates BioLegend citation)
  1. Torres-Nagel N, et al. 1992. Eur. J. Immunol. 22:2841. (IP IHC WB)
  2. Kuhnlein P, et al. 1994. J. Immunol. 153:979. (IHC)
  3. Hirji N, et al. 1997. J. Immunol. 158:1833.
  4. Hirji N, et al. 1998. J. Immunol. 160:6004.
Product Citations
  1. Tang-Huau TL, et al. 2021. Viruses. 13: . PubMed
RRID
AB_313883 (BioLegend Cat. No. 200702)

Antigen Details

Structure
Ig superfamily, CD8β chain
Distribution

Most thymocytes, subset of mature T cells (i.e. including most T suppressor/cytotoxic cells), macrophages

Function
Co-receptor for TCR
Ligand/Receptor
MHC class I molecule
Cell Type
Macrophages, T cells
Biology Area
Immunology
Molecular Family
CD Molecules
Antigen References

1. Torres-Nagel N, et al. 1992. Eur. J. Immunol. 22:2841.
2. Kuhnlein P, et al. 1994. J. Immunol. 153:979.
3. Hirji N, et al. 1997. J. Immunol. 158:1833.
4. Hirji N, et al. 1998. J. Immunol. 160:6004.

Gene ID
24931 View all products for this Gene ID
UniProt
View information about CD8beta on UniProt.org
Go To Top Version: 1    Revision Date: 11.30.2012

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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