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Purified anti-rat RT1A Antibody

Pricing & Availability
Clone
OX-18 (See other available formats)
Regulatory Status
RUO
Other Names
MHC Class I, RT1.A
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
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205201 100 µg 100€
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Description

The rat monomorphic MHC class I antigen RT1A is expressed on nucleated cells (except neurons and glial cells) and erythrocytes. RT1A is involved in antigen presentation to T cells expressing CD3/TCR and CD8 proteins.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Rat
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
RT1B-depleted membrane glycoproteins isolated from Wistar rat spleen
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

FC - Quality tested
IHC-F, IP - Reported in the literature, not verified in house
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Additional reported applications (For the relevant formats) include: immunohistochemical staining of acetone-fixed frozen sections, immnoprecipitation, and blocking function

Application References

(PubMed link indicates BioLegend citation)
  1. Hellstrom M, et al. 2014. Acta Biomater. 10:5034. PubMed
Product Citations
  1. Yu Z, et al. 2018. J Transl Med. 0.902083333. PubMed
  2. Hellström M, et al. 2014. Acta Biomater. 10:5034. PubMed
RRID
AB_1227711 (BioLegend Cat. No. 205201)

Antigen Details

Distribution

Nucleated cells and erythrocytes

Biology Area
Immunology
Molecular Family
MHC Antigens
Gene ID
100188935 View all products for this Gene ID
UniProt
View information about RT1A on UniProt.org
Go To Top Version: 1    Revision Date: 11.30.2012

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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