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Purified anti-SHP-2 Phospho (Tyr542) Antibody

Pricing & Availability
Clone
A22019A (See other available formats)
Regulatory Status
RUO
Other Names
PTPN11, CFC, NS1, JMML, SHP2, BPTP3, PTP2C, METCDS, PTP-1D, SH-PTP2, SH-PTP3, Syp, Protein Tyrosine Phosphatase Non-Receptor Type 11, SH2 Domain-Containing Protein Tyrosine Phosphatase 2
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
A. A22019A_PURE_SHP-2_WB_1_100324
Whole cell extracts (15 µg total protein) from NIH-3T3 cells either untreated (-) or treated (+) with PDGF-BB (Cat. No. 558806) were resolved on a 4-12% Bis-Tris gel, transferred to a PVDF membrane and then probed with Purified anti-SHP-2 Phospho (Tyr542) (clone A22019A) (left blot) or Purified anti-Pan SHP-2 (right blot). Proteins were visualized by chemiluminescence detection using HRP Goat anti-mouse IgG (Cat. No. 405306). Direct-Blot™ HRP anti-GAPDH (Cat. No. 607904) was used as a loading control. Western-Ready™ ECL Substrate Premium Kit (Cat. No. 426318) was used as a detection agent. Lane M: Molecular weight marker
  • A. A22019A_PURE_SHP-2_WB_1_100324
    Whole cell extracts (15 µg total protein) from NIH-3T3 cells either untreated (-) or treated (+) with PDGF-BB (Cat. No. 558806) were resolved on a 4-12% Bis-Tris gel, transferred to a PVDF membrane and then probed with Purified anti-SHP-2 Phospho (Tyr542) (clone A22019A) (left blot) or Purified anti-Pan SHP-2 (right blot). Proteins were visualized by chemiluminescence detection using HRP Goat anti-mouse IgG (Cat. No. 405306). Direct-Blot™ HRP anti-GAPDH (Cat. No. 607904) was used as a loading control. Western-Ready™ ECL Substrate Premium Kit (Cat. No. 426318) was used as a detection agent. Lane M: Molecular weight marker
  • B. A22019A_PURE_SHP-2_WB_2_100324
    Whole cell extracts (15 µg total protein) from A-431 cells untreated (UnTr) or treated with EGF (Cat. No. 585506) for 10, 20, and 30 minutes, were resolved on a 4-12% Bis-Tris gel, transferred to a PVDF membrane and then probed with Purified anti-SHP-2 Phospho (Tyr542) (clone A22019A). Proteins were visualized by chemiluminescence detection using HRP Goat anti-mouse IgG (Cat. No. 405306). Direct-Blot™ HRP anti-GAPDH (Cat. No. 607904) was used as a loading control. Western-Ready™ ECL Substrate Premium Kit (Cat. No. 426318) was used as a detection agent. Lane M: Molecular weight marker
Cat # Size Price Quantity Check Availability Save
651751 25 µg 124€
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651752 100 µg 308€
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Description

SHP-2 is encoded by the PTPN11 gene, is a well-known oncogenic member of the protein tyrosine phosphatase (PTP) family. SHP-2 regulates cellular functions by dephosphorylating targets downstream of PD1, EGFRvIII, HGF receptor, and cytokine receptors. Cellular processes controlled by SHP-2 include mitotic cycle, cell growth, differentiation, and oncogenesis. Phosphorylation of tyrosine 542 and 580 of SHP-2 in response to growth factor receptor activation leads to a stimulation in its tyrosine phosphatase activity. SHP-2 has been recognized as a potential therapeutic target for cancers including breast cancer, lung cancer, leukemia, gastric cancer, and oral cancer.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human, Mouse
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Synthetic peptide of human SHP-2 phosphorylated at Tyr542
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by western blotting. For western blotting, the suggested use of this reagent is 0.25 - 1.0 µg/mL. It is recommended that the reagent be titrated for optimal performance for each application.

Antigen Details

Structure
SHP-2 is a 593 amino acid protein with a molecular mass of 68 kD.
Distribution

Cytoplasm, Nucleus

Function
SHP-2 regulates cell proliferation, apoptosis, invasion, and metastasis
Interaction
SHP-2 interacts with PD1, EGFR, HGF receptor, RET, FGFR2, FGFR3, PI3K/AKT, and TLR3 signaling pathways.
Biology Area
Adaptive Immunity, Cell Biology, Immuno-Oncology, Immunology, Signal Transduction
Molecular Family
Protein Kinases/Phosphatase
Antigen References
  1. Hao C, et al. 2020. Oncogene. 39:7166-7180.
  2. Marie D, et al. 2008. Cell Signal. 20:453-9.
  3. Cheng Kui QU. 2000. Cell Research. 10:279-288.
  4. Bennett AM, et al. 1994. Proc Natl Acad Sci USA. 91:7335.
Gene ID
5781 View all products for this Gene ID
UniProt
View information about SHP-2 Phospho Tyr542 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 10.03.2024

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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