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Purified anti-SUZ12 Antibody

Pricing & Availability
Clone
W22172F (See other available formats)
Regulatory Status
RUO
Other Names
SUZ12 Polycomb Repressive Complex 2 Subunit, Polycomb protein SUZ12, Chromatin precipitated E2F target 9 protein, ChET 9 protein, CHET9, Joined To JAZF1 Protein, JJAZ1, Suppressor Of Zeste 12 Protein Homolog
Isotype
Rat IgG2a, κ
Ave. Rating
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Product Citations
publications
a. W22172C_PURE_SUZ12_WB-1_070824
Whole cell extracts (15 µg total protein per lane) from indicated cell lines were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a PVDF membrane, and probed with purified anti-SUZ12 (clone W22172F) overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP Goat anti-rat IgG (Cat. No. 405405). Direct-Blot™ HRP anti-GAPDH (Cat. No. 607903) was used as a loading control at a 1:50000 dilution. Western-Ready™ ECL Substrate Premium Kit (Cat. No. 426319) was used as a detection agent. Lane M: Molecular weight marker
  • a. W22172C_PURE_SUZ12_WB-1_070824
    Whole cell extracts (15 µg total protein per lane) from indicated cell lines were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a PVDF membrane, and probed with purified anti-SUZ12 (clone W22172F) overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP Goat anti-rat IgG (Cat. No. 405405). Direct-Blot™ HRP anti-GAPDH (Cat. No. 607903) was used as a loading control at a 1:50000 dilution. Western-Ready™ ECL Substrate Premium Kit (Cat. No. 426319) was used as a detection agent. Lane M: Molecular weight marker
  • b. W22172C_PURE_SUZ12_WB-2_070824
    Whole cell extracts (15 µg total protein per lane) from indicated cell lines were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a PVDF membrane, and probed with purified anti-SUZ12 (clone W22172F) overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP Goat anti-rat IgG (Cat. No. 405405). Direct-Blot™ HRP anti-GAPDH (Cat. No. 607903) was used as a loading control at a 1:50000 dilution. Western-Ready™ ECL Substrate Premium Kit (Cat. No. 426319) was used as a detection agent. Lane M: Molecular weight marker
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647051 25 µg 136€
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647052 100 µg 332€
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Description

The SUZ12 protein is a key component of the Polycomb Repressive Complex 2 (PRC2), a major player in gene silencing through histone modification. SUZ12 is essential for the PRC2 complex's ability to methylate histone H3 at lysine 27 (H3K27me3). This methylation marks chromatin for repression, leading to transcriptional silencing of target genes. By contributing to the formation of H3K27me3 marks, SUZ12 helps maintain the repression of genes involved in various cellular processes including development, differentiation, and cell cycle regulation. SUZ12 and the PRC2 complex are crucial for normal embryonic development. They regulate the expression of genes necessary for maintaining pluripotency in embryonic stem cells and for proper lineage commitment during development. Abnormal SUZ12 expression or function are linked to various cancers. SUZ12, as part of PRC2, can act as an oncogene or tumor suppressor, depending on the context. Its deregulation can lead to inappropriate gene silencing or activation, contributing to tumorigenesis.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human, Mouse, Rat
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Recombinant fragment of human SUZ12
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by western blotting. For western blotting, the suggested use of this reagent is 0.25 - 0.5 µg/mL. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

This antibody (clone W22172C) is not suitable for immunocytochemistry (ICC) or immunohistochemistry (IHC).

RRID
AB_3662328 (BioLegend Cat. No. 647051)
AB_3662328 (BioLegend Cat. No. 647052)

Antigen Details

Structure
Contain a C2H2-type Zinc finger domain
Distribution

Located in the nucleus, SUZ12 is overexpressed in breast and colon cancer

Function
A component of Polycomb Repressive Complex 2 (PRC2) which methylates 'Lys-9' (H3K9me) and 'Lys-27' (H3K27me) of histone H3
Interaction
Part of Polycomb Repressive Complex 2 (PRC2); interacts with EED, EZH1, EZH2, JARD2
Biology Area
Chromatin Remodeling/Epigenetics
Antigen References
  1. Pasini D, et al. 2004. EMBO J. 23:4061-71.
  2. Cao R, et al. 2004. Mol Cell. 2004.15:57-67.
  3. Pasini D, et al. 2007. Mol Cell Biol. 2007. 27:3769–3779.
  4. Lee S-R, et al. 2015. Clin Cancer Res. 21:5391-403.
  5. Højfeldt JW, et al. 2018. Nat Struct Mol. Biol. 25:225-232.
Gene ID
23512 View all products for this Gene ID
UniProt
View information about SUZ12 on UniProt.org
Go To Top Version: 1    Revision Date: 07.08.2024

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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