Western blot of purified anti-Synapsin I/II/III antibody (clone A17080A). Lane 1: Molecular weight marker; Lane 2: 20 µg of human brain lysate; Lane 3: 20 µg of rat brain lysate. The blot was incubated with 0.005 µg/mL of the primary antibody overnight at 4°C, followed by incubation with HRP-labeled goat anti-mouse IgG (Cat. No. 405306). Enhanced chemiluminescence was used as the detection system.
Western blot of purified anti-Synapsin I/II/III antibody (clone A17080A). Lane 1: Molecular weight marker; Lane 2: 20 µg of human brain lysate; Lane 3: 20 µg of rat brain lysate. The blot was incubated with 0.005 µg/mL of the primary antibody overnight at 4°C, followed by incubation with HRP-labeled goat anti-mouse IgG (Cat. No. 405306). Enhanced chemiluminescence was used as the detection system.
IHC staining of purified anti-Synapsin I/II/III antibody (clone A17080A) on formalin-fixed paraffin-embedded human cerebellum tissue. Following antigen retrieval using Sodium Citrate H.I.E.R., the tissue was incubated with 0.1 µg/mL of the primary antibody overnight at 4°C. BioLegend´s Ultra Streptavidin (USA) HRP Detection Kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale Bar: 50 µm
IHC staining of purified anti-Synapsin I/II/III antibody (clone A17080A) on formalin-fixed paraffin-embedded rat cerebellum tissue. Following antigen retrieval using Sodium Citrate H.I.E.R., the tissue was incubated with 0.1 µg/mL of the primary antibody overnight at 4°C. BioLegend´s Ultra Streptavidin (USA) HRP Detection Kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale Bar: 50 µm
Synapsins are a family of proteins involved in neurotransmitter release at the chemical synapses. Synapsins anchor synaptic vesicles to the cytoskeletal structures at the presynaptic terminal. Upon receiving an action potential, phosphorylation of synapsins, possibly by PKA, leads to the release of synaptic vesicles from cytoskeletal structures. This allows membrane docking and fusion of synaptic vesicles with the presynaptic membrane and neurotransmitter release.
Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.001 - 0.005 µg per ml. For immunohistochemistry, a concentration range of 0.1 - 0.5 µg/ml is suggested. It is recommended that the reagent be titrated for optimal performance for each application.
Product Citations
Anastas JN, et al. 2020. Cancer Cell. 36(5):528-544.e10.. PubMed
Synapsin I is a 704 amino acid protein with an apparent molecular mass of ~74 kD. Synapsin II is a 582 amino acid protein with an apparent molecular mass of ~63 kD. Synapsin III is a 580 amino acid protein with an apparent molecular mass of ~63 kD.
Distribution
Tissue distribution: Mainly in the central nervous system. Also expressed in testis.
Cellular distribution: Cytosol, nucleus, and plasma membrane.
Function
Synapsins are involved in the regulation of synaptic vesicle fusion and neurotransmitter release at the presynaptic terminals.
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