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Purified anti-TEC Antibody

Pricing & Availability
Clone
W19291E (See other available formats)
Regulatory Status
RUO
Other Names
Tec Protein Tyrosine Kinase, PSCTK4
Isotype
Rat IgG2a, κ
Ave. Rating
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Product Citations
publications
W19291E_PURE_TEC_Antibody_1_121420
Whole cell extracts (15 µg protein) from the indicated cell lines were resolved on a 4-12% Bis-Tris gel, transferred to PVDF and probed with 1.0 µg/mL (1:500 dilution) purified anti-TEC antibody (clone W19291E) overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP goat anti-rat IgG antibody (Cat. No. 405405) at a 1:3000 dilution. Direct-Blot™ HRP anti-β-actin antibody (Cat. No. 643807) was used as a loading control at a 1:10000 dilution (lower). Lane M: Molecular weight marker.
  • W19291E_PURE_TEC_Antibody_1_121420
    Whole cell extracts (15 µg protein) from the indicated cell lines were resolved on a 4-12% Bis-Tris gel, transferred to PVDF and probed with 1.0 µg/mL (1:500 dilution) purified anti-TEC antibody (clone W19291E) overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP goat anti-rat IgG antibody (Cat. No. 405405) at a 1:3000 dilution. Direct-Blot™ HRP anti-β-actin antibody (Cat. No. 643807) was used as a loading control at a 1:10000 dilution (lower). Lane M: Molecular weight marker.
  • W19291E_PURE_TEC_Antibody_2_121420
    Whole cell extracts (250 µg total protein) prepared from HeLa cells were immunoprecipitated overnight with 2.5 µg purified rat IgG2a, κ isotype ctrl antibody (Cat. No. 400502) or purified anti-TEC antibody (clone W19291E). The resulting IP fractions and whole cell extract input (6%) were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a PVDF membrane, and probed with clone W19291E followed by a light chain-specific HRP conjugated secondary anti-rat antibody. Lane M: Molecular weight marker.
  • W19291E_PURE_TEC_Antibody_3_121420
    Total cell lysates (15 µg total protein) from HeLa untreated, treated with non-targeting control siRNA (siCON) or siRNA directed against TEC (siTEC) cells were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a PVDF membrane, and probed with 1.0 µg/mL purified anti-TEC antibody (clone W19291E) overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP goat anti-rat IgG antibody (Cat. No. 405405) at a 1:3000 dilution. Direct-Blot™ HRP anti-GAPDH antibody (Cat. No. 607904) was used as a loading control at a 1:50000 dilution (lower). Lane M: Molecular weight marker.
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944201 25 µg 95€
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944202 100 µg 235€
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Description

TEC is a part of the non-receptor protein tyrosine kinases subfamily which includes Bmx, Itk/Tsk/Emt, Rlk/Txk, and Btk proteins. As a non-receptor protein kinase, TEC contributes to signaling from many receptors and acts as a signal transducer in multiple downstream pathways, this includes the regulation of the actin cytoskeleton. The enzyme’s primary location in mammalian tissues is the plasma membrane. It is involved in regulating the development, function and differentiation of conventional T-cells and nonconventional NKT-cells.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Partial recombinant human TEC protein
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
KO/KD-WB, IP – Verified
Recommended Usage

Each lot of this antibody is quality control tested by western blotting. For western blotting, the suggested use of this reagent is 0.5 - 1.0 µg/mL. For immunoprecipitation, the suggested use of this reagent is 2.5 µg/test. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

This clone was tested for ICC using HeLa cells treated with control siRNA or siRNA targeting TEC. While staining was observed, it was not responsive to TEC protein depletion using siRNA, indicating the staining was non-specific. We therefore do not recommend this clone for ICC.

RRID
AB_2890870 (BioLegend Cat. No. 944201)
AB_2890870 (BioLegend Cat. No. 944202)

Antigen Details

Structure
TEC is a 631 amino acid protein with a predicted molecular weight of 74 kD.
Distribution

Broadly expressed/Plasma membrane-associated, cytoplasm.

Biology Area
Signal Transduction
Molecular Family
Protein Kinases/Phosphatase, TCRs
Antigen References
  1. Yang WC, et al. 1999. J Biol Chem. 274:607-17.
  2. Susaki K, et al. 2010. Immunol Lett. 127:135-42.
  3. Yang WC, et al. 2001. J Immunol. 166:387-95.
Gene ID
7006 View all products for this Gene ID
UniProt
View information about TEC on UniProt.org

Related FAQs

There are no FAQs for this product.

Other Formats

View All TEC Reagents Request Custom Conjugation
Description Clone Applications
Purified anti-TEC W19291E WB,KO/KD-WB,IP
Go To Top Version: 2    Revision Date: 04.13.2021

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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