- Clone
- 2T5C9 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- PALB, ATTR, Prealbumin, TBPA, TTR
- Isotype
- Mouse IgG1, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
![2T5C9_PURE_Transthyretin_CAPTUREELISA_010317_resized 2T5C9_PURE_Transthyretin_CAPTUREELISA_010317_resized](https://d1spbj2x7qk4bg.cloudfront.net/Admin/Public/GetImage.ashx?Image=/Files/Images/media_assets/products/product_images/2T5C9_PURE_Transthyretin_CAPTUREELISA_010317_resized.jpg&Width=240&Height=300&altFmImage_path=&Compression=90&Crop=5)
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Capture ELISA of purified anti-Transthyretin, aggregated antibody (clone 2T5C9) binding to soluble aggregates of recombinant human TTR . ELISA was performed by coating wells with 100 ng of clone 2T5C9. The wells were then incubated at 37°C for 1h with serially diluted (0.005 to 10 µg/ml) soluble TTR aggregates, followed by incubation with horseradish peroxidase labeled goat anti-TTR secondary antibody. TMB (3, 3', 5, 5' tetramethylbenzidine) was used as the detection system (Cat. No. 421501). -
Congo red (A) and purified anti-Transthyretin, aggregated antibody (clone 2T5C9) (B) staining of Transtheyretin amyloid deposits in formalin-fixed paraffin-embedded heart tissue of a patient with familial amyloidotic polyneuropathy (FAP). Following antigen retrieval using Antigen Retrieval Citra Plus (BioGenex), the tissue was incubated with the primary antibody at 3 µg/mL overnight at 4°C. Horse radish peroxidase conjugated anti-mouse IgG was used as the detection system.
Cat # | Size | Price | Quantity Check Availability | Save | ||
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848201 | 25 µg | 90€ | ||||
848202 | 100 µg | 231€ |
Transthyretin (TTR) is a carrier protein capable of transporting thyroid hormones in the plasma and cerebrospinal fluid, and retinol (vitamin A) in the plasma. TTR can form aberrant, insoluble amyloid fibrils that primarily deposit in a patient's heart, peripheral nerves, or both. The wild-type protein is associated with senile systemic amyloidosis (SSA) and is typically diagnosed in men over 70-years-old. Single or double mutations in TTR are primarily associated with familial amyloidotic polyneuropathy (FAP) or familial amyloid cardiopathy (FAC) which affects younger patients, often in their 30s. Clinical symptoms of TTR amyloidosis have historically been attributed to fibrils, but recent experimental evidence indicates that soluble TTR aggregates are also pathogenic.
Product DetailsProduct Details
- Verified Reactivity
- Human
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- A 1:1 mixture of wild-type Transthyretin fibrils and soluble aggregates of Y78F TTR.
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography.
- Concentration
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C.
- Application
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ELISA Capture - Quality tested
IHC-P - Verified
Direct ELISA - Reported in the literature, not verified in house - Recommended Usage
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Each lot of this antibody is quality control tested by ELISA assay. For ELISA capture applications, a concentration of 2.0 µg/ml is recommended. For immunohistochemical staining on formalin-fixed paraffin-embedded tissue sections, a concentration of is 1.0 - 5.0 µg/ml is recommmended. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
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Clone 2T5C9 recognizes a mid-region epitope on transthyretin aggregates.
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Application References
(PubMed link indicates BioLegend citation) -
- Phay M, et al. 2014. Rejuvenation Res. 17(2):97. (Direct ELISA, Capture ELISA, IHC) PubMed
- RRID
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AB_2632817 (BioLegend Cat. No. 848201)
AB_2632818 (BioLegend Cat. No. 848202)
Antigen Details
- Structure
- Transthyretin (TTR) is a β-sheet rich homotetrameric protein that has a molecular mass of 55 kD. Each monomer consists of 127 amino acids.
- Distribution
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Primarily extracellular in blood and cerebrospinal fluid.
- Function
- The normal function of TTR is to transport thyroxine and retinol-binding protein in the blood and cerebrospinal fluid.
- Interaction
- Perlecan, receptor for advanced glycation end product (RAGE).
- Ligand/Receptor
- Thyroxine, retinol-binding protein bound to retinol.
- Cell Sources
- Liver, choroid plexus epithelium, retina pigment epithelium.
- Biology Area
- Cell Biology, Neurodegeneration, Neuroscience, Protein Misfolding and Aggregation
- Antigen References
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1. Plante-Bordeneuve V, Said G. 2011. Lancet Neurol. 10:1086. PubMed
2. Pinney JH, et al. 2013. J. Am. Heart Assoc. 2:e000098. PubMed
3. Hawkins PN, et al. 2015. Ann Med. 47(8):625. PubMed - Gene ID
- 7276 View all products for this Gene ID
- UniProt
- View information about Transthyretin on UniProt.org
Related Pages & Pathways
Pages
Other Formats
View All Transthyretin Reagents Request Custom ConjugationDescription | Clone | Applications |
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Purified anti-Transthyretin, aggregated | 2T5C9 | ELISA Capture,IHC-P,Direct ELISA |
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Purified anti-Transthyretin, aggregated
Capture ELISA of purified anti-Transthyretin, aggregated ant... Congo red (A) and purified anti-Transthyretin, aggreg...
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