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Purified anti-VCP Antibody

Pricing & Availability
Clone
A18143C (See other available formats)
Regulatory Status
RUO
Other Names
Valosin-Containing Protein, CDC48
Isotype
Mouse IgG2b, κ
Ave. Rating
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Product Citations
publications
A18143C_PURE_VCP_Antibody_1_021920
Western blot of purified anti-VCP antibody (clone A18143C). Lane 1: Molecular weight marker; Lane 2: 20 µg of human brain lysate; Lane 3: 20 µg of mouse brain lysate; Lane 4: 20 µg of rat brain lysate. The blot was incubated with 2 µg/mL of the primary antibody overnight at 4°C, followed by incubation with HRP goat anti-mouse IgG antibody (Cat. No. 405306). Enhanced chemiluminescence was used as the detection system.
  • A18143C_PURE_VCP_Antibody_1_021920
    Western blot of purified anti-VCP antibody (clone A18143C). Lane 1: Molecular weight marker; Lane 2: 20 µg of human brain lysate; Lane 3: 20 µg of mouse brain lysate; Lane 4: 20 µg of rat brain lysate. The blot was incubated with 2 µg/mL of the primary antibody overnight at 4°C, followed by incubation with HRP goat anti-mouse IgG antibody (Cat. No. 405306). Enhanced chemiluminescence was used as the detection system.
  • A18143C_PURE_VCP_Antibody_2_021920
    IHC staining of purified anti-VCP antibody (clone A18143C) on formalin-fixed paraffin-embedded rat brain tissue. Following antigen retrieval using Sodium Citrate H.I.E.R., the tissue was incubated with 2 µg/mL of the primary antibody overnight at 4°C. BioLegend's Ultra Streptavidin (USA) HRP Detection Kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale bar: 50 µm
  • A18143C_PURE_VCP_Antibody_3_021920
    IHC staining of purified anti-VCP antibody (clone A18143C) on formalin-fixed paraffin-embedded mouse brain tissue. Following antigen retrieval using Sodium Citrate H.I.E.R., the tissue was incubated with 2 µg/mL of the primary antibody overnight at 4°C. BioLegend's Ultra Streptavidin (USA) HRP Detection Kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale bar: 50 µm
Cat # Size Price Quantity Check Availability Save
870901 25 µg 85€
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870902 100 µg 221€
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Description

Valocin-containing protein (VCP) is a member of a family of proteins that include putative ATP-binding proteins involved in vesicle transport and fusion, 26S proteasome function, and assembly of peroxisomes. VCP associates with clathrin and heat-shock protein Hsc70 to form a complex that functions as a chaperon to unfold and transport proteins to different cellular compartments. Diseases associated with VCP include inclusion body myopathy with early-onset Paget's disease, and frontotemporal dementia 1 and amyotrophic lateral sclerosis 14, with or without frontotemporal dementia.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human, Mouse, Rat
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Recombinant Human VCP Protein
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
IHC-P - Verified
Recommended Usage

Each lot of this antibody is quality control tested by western blotting. For western blotting, the suggested use of this reagent is 0.5 - 2.0 µg/mL. For immunohistochemistry on formalin-fixed paraffin-embedded tissue sections, a concentration range of 0.5 - 10 µg/mL is suggested. It is recommended that the reagent be titrated for optimal performance for each application.

RRID
AB_2861105 (BioLegend Cat. No. 870901)
AB_2861105 (BioLegend Cat. No. 870902)

Antigen Details

Structure
VCP is an 806 amino acid protein with predicted molecular mass of ~89 kD and observed molecular mass at ~ 97 kD.
Distribution

Tissue Distribution: Ubiquitously expressed
Cellular Distribution: Cytosol, endoplasmic reticulum, nucleus, extracellular, and lysosome

Function
Protein Trafficking, protein clearance
Interaction
Forms homohexamers and a ternary complex with STX5A and NSFLC. Interacts with NPLOC4, UFD1, and clathrin.
Cell Type
Astrocytes, Dopaminergic Neurons, Endothelial cells, Epithelial cells, GABAergic Neurons, Glutamatergic Neurons, Mature Neurons, Microglia, Neural Stem Cells, Neurons, Oligodendrocytes
Biology Area
Cell Motility/Cytoskeleton/Structure, Neurodegeneration, Neuroinflammation, Neuroscience, Protein Misfolding and Aggregation, Protein Trafficking and Clearance, Signal Transduction, Ubiquitin/Protein Degradation
Molecular Family
Endoplasmic Reticulum Markers, Neurotransmitter Receptor Trafficking/Endocytosis, Synaptic Vesicle Trafficking/Endocytosis
Antigen References
  1. Lan B, et al. 2017. Curr Mol Med. 17(9):608.
  2. van den Boom J & Meyer H. 2018. Mol Cell. 69(2):182.
  3. Van Mossevelde S, et al. 2018. Nat Rev Neurol. 14(6):363.
Gene ID
7415 View all products for this Gene ID 269523 View all products for this Gene ID 116643 View all products for this Gene ID
UniProt
View information about VCP on UniProt.org
Go To Top Version: 1    Revision Date: 02.19.2020

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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