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Purified Mouse IgG2a, κ Isotype Ctrl Antibody

Pricing & Availability
Clone
MG2a-53 (See other available formats)
Regulatory Status
RUO
Isotype
Mouse IgG2a, κ
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Product Citations
publications
MG2a-53_GoChIP_IgG2a_Iso-Con_060618_updated.png
Chromatin Immunoprecipitations (ChIP) were performed with cross-linked chromatin samples from 4 X 106 of Jurkat cells with either A)1:100 dilution of Go-ChIP-Grade™ Purified anti-human Ikaros (Clone 16B5C71, Cat. No. 687103) or B) equal amount of Go-ChIP-Grade™ Purified Mouse IgG2a, κ Isotype Control Antibody (Clone MG2a-53, Cat. No. 401505) by using Go-ChIP-Grade™ Protein G Enzymatic Kit (Cat. No. 699904). The enriched DNA was purified and quantified by real-time qPCR using primers targeting human Hes1 gene region and α-Satellite repeats. The amount of immunoprecipitated DNA in each sample is represented as signal relative to total amount of input chromatin.
  • MG2a-53_GoChIP_IgG2a_Iso-Con_060618_updated.png
    Chromatin Immunoprecipitations (ChIP) were performed with cross-linked chromatin samples from 4 X 106 of Jurkat cells with either A)1:100 dilution of Go-ChIP-Grade™ Purified anti-human Ikaros (Clone 16B5C71, Cat. No. 687103) or B) equal amount of Go-ChIP-Grade™ Purified Mouse IgG2a, κ Isotype Control Antibody (Clone MG2a-53, Cat. No. 401505) by using Go-ChIP-Grade™ Protein G Enzymatic Kit (Cat. No. 699904). The enriched DNA was purified and quantified by real-time qPCR using primers targeting human Hes1 gene region and α-Satellite repeats. The amount of immunoprecipitated DNA in each sample is represented as signal relative to total amount of input chromatin.
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401501 50 µg 38€
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401502 500 µg 118€
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Description

The isotype of MG2a-53 immunoglobulin is mouse IgG2a,κ. This antibody was chosen as an isotype control after screening on a variety of resting, activated, live, and fixed mouse, rat and human tissues.

Product Details
Technical Data Sheet (pdf)

Product Details

Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Trinitrophenol + KLH
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The immunoglobulin was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The immunoglobulin solution should be stored undiluted between 2°C and 8°C
Application

FC - Quality tested
ChIP - Verified
ICFC, WB, IP, ICC, IHC, FA - Reported in the literature, not verified in house
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis as negative control, and the purity is greater than 95% by SDS-PAGE. Use at concentrations comparable to those of the specific antibody of interest.

Application Notes

Additional reported applications (for the relevant formats) include: Western blotting, immunoprecipitation, immunocytochemistry, immunohistochemistry, and functional assay. The Ultra-LEAF™ purified antibody (Endotoxin <0.1 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. Nos. 401507-401512).

Application References

(PubMed link indicates BioLegend citation)
  1. Bhattacharyya S, et al. 2007. Am. J. Physiol. Gastrointest Liver Physiol. doi:10.1152/ajpgi.00149.
  2. Lee H, et al. 2014. Invest. Ophthalmol. Vis. Sci. 55:2885. PubMed
  3. Baker GF, et al. 2014. Cancer Res. 74:5079. PubMed
Product Citations
  1. Shah D, et al. 2021. Oncoimmunology. 10:1939601. PubMed
  2. Vitry J, et al. 2021. Int J Mol Sci. 22:. PubMed
  3. Saleh KK, et al. 2022. iScience. 25:105415. PubMed
  4. Ogura H, et al. 2022. Nat Commun. 13:7063. PubMed
  5. Frasca D, et al. 2020. Front Immunol. 11:392. PubMed
  6. Zhang SY, et al. 2023. Signal Transduct Target Ther. 8:236. PubMed
  7. Baker G, et al. 2014. Cancer Res. 74:5079. PubMed
  8. Gu C, et al. 2019. J Immunol. 203:389. PubMed
  9. Bradford BM, et al. 2018. Parasite Immunol. 40:e12566. PubMed
  10. Lee H, et al. 2014. Invest Ophthalmol Vis Sci . 55:2885. PubMed
  11. Wallis J, et al. 2020. Eur J Pharm Sci. 152:105456. PubMed
  12. Wang J, et al. 2020. Cell. 183:1264. PubMed
  13. Narayan S, et al. 2022. iScience. 25:104518. PubMed
  14. Yamada S, et al. 2021. J Tissue Eng. 12:20417314211019375. PubMed
  15. Liu PY, et al. 2019. Nat Commun. 3.906944444. PubMed
  16. Parra-Sánchez H, et al. 2018. Mol Immunol. 96:1. PubMed
  17. Xu L, et al. 2020. Nat Cell Biol. 1162:22. PubMed
  18. Ghosh R, et al. 2018. PLoS One. 13:e0194611. PubMed
  19. Paré G, et al. 2021. Front Immunol. 12:650808. PubMed
  20. Dunham C, et al. 2020. FASEB J. 34:12976. PubMed
  21. Smith TC, et al. 2022. Skelet Muscle. 12:2. PubMed
  22. Zhou J, et al. 2020. Nat Commun. 0.625. PubMed
RRID
AB_2800437 (BioLegend Cat. No. 401501)
AB_2800437 (BioLegend Cat. No. 401502)

Antigen Details

Gene ID
NA

Related FAQs

IgG2a gene is deleted in some mouse strains, which ones are they?

IgG2a gene is deleted in C57Bl/6, C57Bl/10, SJL, and NOD mice. It is replaced with IgG2c gene instead.

Other Formats

View All Reagents
Description Clone Applications
Purified Mouse IgG2a, κ Isotype Ctrl MG2a-53 FC,ICFC,WB,IP,ICC,IHC,FA,ChIP
Ultra-LEAF™ Purified Mouse IgG2a, κ Isotype Ctrl MG2a-53 FC,ICFC,WB,IP,ICC,IHC,FA
TotalSeq™-Bn1364 Mouse IgG2a, κ Isotype Ctrl MG2a-53 SB
Go To Top Version: 2    Revision Date: 08.24.2021

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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