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- Regulatory Status
- RUO
- Other Names
- CD44 antigen, Extracellular matrix receptor III, ECMR-III, GP90 lymphocyte homing/adhesion receptor, HUTCH-I, Hermes antigen, Hyaluronate receptor, Lymphocyte antigen 24, Ly-24, Phagocytic glycoprotein 1, PGP-1, gp85, H-CAM
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- Product Citations
- publications
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Immobilized recombinant mouse CD44 at 0.5 µg/mL binds biotinylated hyaluronan in a dose-dependent manner. The ED50 for this effect is 6 - 36 ng/mL. -
Stability Testing for Recombinant Mouse CD44. Recombinant Mouse CD44 was aliquoted in PBS, pH 7 at 0.2 mg/mL. One aliquot was frozen and thawed four times (4x Freeze/Thaw) and compared to the control that was kept at 4°C (Control). The samples were tested for their ability to bind biotinylated hyaluronan in a dose-dependent manner using a functional ELISA. The ED50 for this effect is 6 - 36 ng/mL.
CD44, also known as Hermes antigen, PGP-1, H-CAM, or HUTCH-I, is an 80-95 kD glycoprotein. CD44 is a major receptor for hyaluronan (HA). Other ligands to CD44 were identified in the extracellular matrix (ECM), such as fibronectin, laminin and type I collagen. CD44 is expressed on all leukocytes, endothelial cells, hepatocytes, and mesenchymal cells. As B and T cells become activated or progress to the memory stage, CD44 expression increases from low or medium levels to high levels. Thus, CD44 has been reported to be a valuable marker for memory cell subsets. High CD44 expression on Treg cells has been associated with potent suppressive function via high production of IL-10. CD44 is an adhesion molecule involved in leukocyte attachment to and rolling on endothelial cells, homing to peripheral lymphoid organs and to sites of inflammation and leukocyte aggregation. CD44 associates with ezrin/radixin/moesin (ERM) proteins and serves as a platform to link the extracellular matrix and the actin cytoskeleton. Membrane-type I matrix metalloproteinase (MT1-MMP) ADAM-like protease mediates constitutive shedding of the common form of CD44 in many types of cells and human tumor tissues. Proteolytic cleavage of the ectodomain of CD44 is induced by various stimulations, such as calcium influx and the activation of PKC, Rac and Ras oncoproteins. Release of the soluble form of the ectodomain is responsible for the regulation of the interaction of CD44 and the ECM during cell adhesion, migration, and invasion.
Product DetailsProduct Details
- Source
- Mouse CD44, amino acid (Gln25-Thr224) (Accession: #NP_033981.2), with a linker (GSSR) and a C-terminal human IgG1 (Pro100-Lys330), was expressed in CHO cells.
- Molecular Mass
- The 435 amino acid recombinant protein has a predicted molecular mass of approximately 48.48 kD. The DTT-reduced and non-reduced protein migrates at approximately 70 kD and 140 kD by SDS-PAGE, respectively. The predicted N-terminal amino acid is Gln.
- Purity
- >95%, as determined by Coomassie stained SDS-PAGE.
- Formulation
- 0.22 µm filtered protein solution is in PBS.
- Endotoxin Level
- Less than 0.01 ng per µg cytokine as determined by the LAL method.
- Concentration
- 10 and 25 µg sizes are bottled at 200 µg/mL. 100 µg size and larger sizes are lot-specific and bottled at the concentration indicated on the vial. To obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.
- Storage & Handling
- Unopened vial can be stored between 2°C and 8°C for up to 2 weeks, at -20°C for up to six months, or at -70°C or colder until the expiration date. For maximum results, quick spin vial prior to opening. The protein can be aliquoted and stored at -20°C or colder. Stock solutions can also be prepared at 50 - 100 µg/mL in appropriate sterile buffer, carrier protein such as 0.2 - 1% BSA or HSA can be added when preparing the stock solution. Aliquots can be stored between 2°C and 8°C for up to one week and stored at -20°C or colder for up to 3 months. Avoid repeated freeze/thaw cycles.
- Activity
- Mouse CD44 binds biotinylated hyaluronan with an EC50 of 15-60 ng/mL in a functional ELISA when immobilized at 0.5 µg/mL.
- Application
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Bioassay
- Application Notes
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BioLegend carrier-free recombinant proteins provided in liquid format are shipped on blue ice. Our comparison testing data indicates that when handled and stored as recommended, the liquid format has equal or better stability and shelf-life compared to commercially available lyophilized proteins after reconstitution. Our liquid proteins are verified in-house to maintain activity after shipping on blue ice and are backed by our 100% satisfaction guarantee. If you have any concerns, contact us at tech@biolegend.com.
Antigen Details
- Structure
- Homodimer
- Distribution
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All leukocytes, epithelial cells, endothelial cells, hepatocytes, mesenchymal cells; plasma membrane.
- Function
- Leukocyte attachment and rolling on endothelial cells, stromal cells and ECM.
- Interaction
- CD44 interacts with HIF-2α and improves HIF target gene activation.
- Ligand/Receptor
- Hyaluronan, MIP-1β, fibronectin, collagen, laminin, and other glycosaminoglycans.
- Bioactivity
- Immobilized recombinant mouse CD44 at 0.5 µg/mL binds biotinylated hyaluronan in a dose-dependent manner.
- Cell Type
- B cells, Granulocytes, T cells, Tregs
- Biology Area
- Cell Adhesion, Cell Motility/Cytoskeleton/Structure, Immuno-Oncology, Immunology
- Molecular Family
- Adhesion Molecules, Blood Group Markers, Soluble Receptors
- Antigen References
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- Melrose J, et al. 1996. Electrophoresis. 17:205-12.
- Yang B, et al. 1995. Anal. Biochem. 228:299-306.
- Yonemura S, et al. 1998. J. Cell. Biol. 140:885.
- Bourguignon LY, et al. 2007. J. Neurochem. 101:1002-17.
- Nakamura H, et al. 2004. Cancer Res. 64:876-82.
- Nagano O, Saya H. 2004. Cancer Sci. 95:930-35.
- Legg JW, et al. 2002. Nat. Cell. Biol. 4:399-407.
- Johansson E, et al. 2017. Cell. Rep. 20:1641-1653.
- Gene ID
- 12505 View all products for this Gene ID
- UniProt
- View information about CD44 on UniProt.org
Related FAQs
- Why choose BioLegend recombinant proteins?
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• Each lot of product is quality-tested for bioactivity as indicated on the data sheet.
• Greater than 95% Purity or higher, tested on every lot of product.
• 100% Satisfaction Guarantee for quality performance, stability, and consistency.
• Ready-to-use liquid format saves time and reduces challenges associated with reconstitution.
• Bulk and customization available. Contact us.
• Learn more about our Recombinant Proteins. - How does the activity of your recombinant proteins compare to competitors?
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We quality control each and every lot of recombinant protein. Not only do we check its bioactivity, but we also compare it against other commercially available recombinant proteins. We make sure each recombinant protein’s activity is at least as good as or better than the competition’s. In order to provide you with the best possible product, we ensure that our testing process is rigorous and thorough. If you’re curious and eager to make the switch to BioLegend recombinants, contact your sales representative today!
- What is the specific activity or ED50 of my recombinant protein?
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The specific activity range of the protein is indicated on the product datasheets. Because the exact activity values on a per unit basis can largely fluctuate depending on a number of factors, including the nature of the assay, cell density, age of cells/passage number, culture media used, and end user technique, the specific activity is best defined as a range and we guarantee the specific activity of all our lots will be within the range indicated on the datasheet. Please note this only applies to recombinants labeled for use in bioassays. ELISA standard recombinant proteins are not recommended for bioassay usage as they are not tested for these applications.
- Have your recombinants been tested for stability?
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Our testing shows that the recombinant proteins are able to withstand room temperature for a week without losing activity. In addition the recombinant proteins were also found to withstand four cycles of freeze and thaw without losing activity.
- Does specific activity of a recombinant protein vary between lots?
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Specific activity will vary for each lot and for the type of experiment that is done to validate it, but all passed lots will have activity within the established ED50 range for the product and we guarantee that our products will have lot-to-lot consistency. Please conduct an experiment-specific validation to find the optimal ED50 for your system.
- How do you convert activity as an ED50 in ng/ml to a specific activity in Units/mg?
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Use formula Specific activity (Units/mg) = 10^6/ ED50 (ng/mL)
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