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Spark Blue™ 515 anti-human TNF-α Antibody

Pricing & Availability
Clone
W19063E (See other available formats)
Regulatory Status
RUO
Other Names
Tumor necrosis factor-α, Cachectin, Necrosin, Macrophage cytotoxic factor (MCF), Differentiation inducing factor (DIF), TNFSF2
Isotype
Rat IgG1, κ
Ave. Rating
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Product Citations
publications
W19063E_Spark-blue-515_TNF-A_Ab_012324
Human peripheral blood lymphocytes were left un-stimulated (right) or stimulated with Cell Activation Cocktail (with Brefeldin A) (Cat. No. 423303) for 6 hours (left) were surface stained with anti-human CD3 (clone UCHT1) Brilliant Violet 421™. Cells were then fixed, permeabilized, and intracellularly stained with anti-human TNF-α (clone W19063E) Spark Blue™ 515. Data shown is gated on the lymphocyte population.
  • W19063E_Spark-blue-515_TNF-A_Ab_012324
    Human peripheral blood lymphocytes were left un-stimulated (right) or stimulated with Cell Activation Cocktail (with Brefeldin A) (Cat. No. 423303) for 6 hours (left) were surface stained with anti-human CD3 (clone UCHT1) Brilliant Violet 421™. Cells were then fixed, permeabilized, and intracellularly stained with anti-human TNF-α (clone W19063E) Spark Blue™ 515. Data shown is gated on the lymphocyte population.
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376217 25 tests 137€
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376218 100 tests 296€
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Description

TNF-α is secreted by macrophages, monocytes, neutrophils, T cells (principally CD4+), and NK cells. Many transformed cell lines also secrete TNF-α. Monomeric human TNF-α is a 157 amino acid protein (non-glycosylated) with a reported molecular weight of 17 kD. TNF-α forms multimeric complexes; stable trimers are most common in solution. A 26 kD membrane form of TNF-α has also been described. TNF-α binding to surface receptors elicits a wide array of biological activities including: cytolysis and cytostasis of many tumor cell lines in vitro, hemorraghic necrosis of tumors in vivo, increased fibroblast proliferation, and enhanced chemotaxis and phagocytosis in neutrophils.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Rat
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography and conjugated with Spark Blue™ 515 under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

ICFC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by intracellular flow cytometry using our Cyto-Fast™ Fix/Perm Buffer Set. For flow cytometric staining, the suggested use of this reagent is 5 µL per 10^6 cells in 100 µL volume. It is highly recommended that the reagent be titrated for optimal performance for each application.

* Spark Blue™ 515 has a maximum excitation of 490 nm and a maximum emission of 515 nm.

Excitation Laser
Blue Laser (488 nm)
RRID
AB_3097529 (BioLegend Cat. No. 376217)
AB_3097529 (BioLegend Cat. No. 376218)

Antigen Details

Structure
TNF superfamily; dimer/trimer; 17 kD (Mammalian)
Distribution

Activated monocytes, neutrophils, macrophages, T cells, B cells, NK cells, LAK cells

Function
Type II integral membrane protein processed by TACE for secretion; upregulated by interferons, IL-2, GM-CSF, substance P, bradykinin, PAF, immune complexes, cyclooxygenase; downregulated by IL-6, TGF-β, vitamin D3, prostaglandin E2, PAF antagonists
Interaction
Monocytes, neutrophils, macrophages, T cells, fibroblasts, endothelial cells, osteoclasts, adipocytes, astroglia, microglia
Ligand/Receptor
TNFRSF1A (TNF-R1, CD120a, TNFR-p60 Type β, p55); TNFRSF1B (TNF-R2, CD120b, TNFR-p80 Type A, p75)
Cell Type
B cells, Granulocytes, Lymphocytes, Macrophages, Monocytes, NK cells, NKT cells, T cells, Th1, Th17
Biology Area
Adaptive Immunity, Cell Death, Immunology, Innate Immunity
Molecular Family
Cytokines/Chemokines
Antigen References
  1. Volova L.T, et al. 2020. Biomolecules. 10:1563.
  2. Szondy Z, et al. 2017. Pharmacological Research. 115:124-132.
Gene ID
7124 View all products for this Gene ID
UniProt
View information about TNF-alpha on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 01.11.2024

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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