Spark Blue™ 515 anti-mouse CD8a Antibody, CD8a, 53-6.7

Pricing & Availability

Clone: 53-6.7 (See other available formats)
Regulatory Status: RUO
Other Names: T8, Lyt2, Ly-2
Isotype: Rat IgG2a, κ
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Product Citations: publications

53-67_Spark-Blue-515_CD8a_Antibody_041823_2 — C57BL/6 mouse splenocytes were stained with anti-mouse CD3ε APC and anti-mouse CD8a (clone 53-6.7) Spark Blue™ 515 (left) or stained with anti-mouse CD3ε APC only (right).

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104005

25 µg

132€

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104006

100 µg

300€

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Description

CD8, also known as Lyt-2, Ly-2, or T8, consists of disulfide-linked α and β chains that form the α(CD8a)/β(CD8b) heterodimer and α/α homodimer. CD8a is a 34 kD protein that belongs to the immunoglobulin family. The CD8 α/β heterodimer is expressed on the surface of most thymocytes and a subset of mature TCR α/β T cells. CD8 expression on mature T cells is non-overlapping with CD4. The CD8 α/α homodimer is expressed on a subset of γ/δ TCR-bearing T cells, NK cells, intestinal intraepithelial lymphocytes, and lymphoid dendritic cells. CD8 is an antigen co-receptor on T cells that interacts with MHC class I on antigen-presenting cells or epithelial cells. CD8 promotes T cell activation through its association with the TCR complex and protein tyrosine kinase lck.

Product Details

Technical Data Sheet (pdf)

Product Details

Verified Reactivity: Mouse
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: Mouse thymus or spleen
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation: The antibody was purified by affinity chromatography and conjugated with Spark Blue™ 515 under optimal conditions.
Concentration: 0.5 mg/mL
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.

* Spark Blue™ 515 has a maximum excitation of 490 nm and a maximum emission of 515 nm.
Excitation Laser: Blue Laser (488 nm)
Application Notes: Clone 53-6.7 antibody competes with clone 5H10-1 antibody for binding to thymocytes³. The 53-6.7 antibody has been reported to block antigen presentation via MHC class I and inhibit T cell responses to IL-2. This antibody has also been used for depletion of CD8a⁺ cells. Additional reported applications (for the relevant formats) include: immunoprecipitation^1,3, in vivo and in vitro cell depletion^2,10,15, inhibition of CD8 T cell proliferation³, blocking of cytotoxicity^3,4, immunohistochemical staining^5,6 of acetone-fixed frozen sections and zinc-fixed paraffin-embedded sections, and spatial biology (IBEX)^29,30. Clone 53-6.7 is not recommended for immunohistochemistry of formalin-fixed paraffin sections. The Ultra-LEAF™ purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays or in vivo studies (Cat No. 100746).
RRID: AB_2941374 (BioLegend Cat. No. 104005)
AB_2941374 (BioLegend Cat. No. 104006)

Antigen Details

Structure: Ig superfamily, CD8α chain, 34 kD
Distribution: Most thymocytes, T cell subset, some NK cells, lymphoid dendritic cells
Function: Co-receptor for TCR
Ligand/Receptor: MHC class I molecule
Antigen References: 1. Barclay A, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Zamoyska R. 1994. Immunity 1:243.
3. Ellmeier W, et al. 1999. Annu. Rev. Immunol. 17:523.
Gene ID: 12525 View all products for this Gene ID
UniProt: View information about CD8alpha on UniProt.org

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Other Formats

View All CD8a Reagents Request Custom Conjugation

Description	Clone	Applications
APC anti-mouse CD8a	53-6.7	FC
Biotin anti-mouse CD8a	53-6.7	FC,IHC
FITC anti-mouse CD8a	53-6.7	FC
PE anti-mouse CD8a	53-6.7	FC
PE/Cyanine5 anti-mouse CD8a	53-6.7	FC
Purified anti-mouse CD8a	53-6.7	FC,CyTOF®,IHC-F,IP
PE/Cyanine7 anti-mouse CD8a	53-6.7	FC
APC/Cyanine7 anti-mouse CD8a	53-6.7	FC
Alexa Fluor® 488 anti-mouse CD8a	53-6.7	FC,3D IHC
Alexa Fluor® 647 anti-mouse CD8a	53-6.7	FC,IHC-F,3D IHC,SB
Pacific Blue™ anti-mouse CD8a	53-6.7	FC
Alexa Fluor® 700 anti-mouse CD8a	53-6.7	FC
PerCP/Cyanine5.5 anti-mouse CD8a	53-6.7	FC
PerCP anti-mouse CD8a	53-6.7	FC
Brilliant Violet 421™ anti-mouse CD8a	53-6.7	FC,IHC,SB
Brilliant Violet 570™ anti-mouse CD8a	53-6.7	FC
Brilliant Violet 650™ anti-mouse CD8a	53-6.7	FC
Brilliant Violet 605™ anti-mouse CD8a	53-6.7	FC
Ultra-LEAF™ Purified anti-mouse CD8a	53-6.7	FC,CyTOF®,IHC,IP,Depletion,Block
Brilliant Violet 711™ anti-mouse CD8a	53-6.7	FC
Brilliant Violet 785™ anti-mouse CD8a	53-6.7	FC
Brilliant Violet 510™ anti-mouse CD8a	53-6.7	FC
Purified anti-mouse CD8a (Maxpar® Ready)	53-6.7	FC,CyTOF®
Alexa Fluor® 594 anti-mouse CD8a	53-6.7	IHC-F,FC,3D IHC
PE/Dazzle™ 594 anti-mouse CD8a	53-6.7	FC
APC/Fire™ 750 anti-mouse CD8a	53-6.7	FC
GoInVivo™ Purified anti-mouse CD8a	53-6.7	FC
TotalSeq™-A0002 anti-mouse CD8a	53-6.7	PG
Spark Blue™ 550 anti-mouse CD8a	53-6.7	FC
Spark NIR™ 685 anti-mouse CD8a	53-6.7	FC
TotalSeq™-C0002 anti-mouse CD8a	53-6.7	PG
TotalSeq™-B0002 anti-mouse CD8a	53-6.7	PG
Spark YG™ 570 anti-mouse CD8a	53-6.7	IHC-F
PE/Fire™ 640 anti-mouse CD8a	53-6.7	FC
PE/Fire™ 700 anti-mouse CD8a	53-6.7	FC
Spark Blue™ 574 anti-mouse CD8a Antibody	53-6.7	FC
Spark Violet™ 423 anti-mouse CD8a Antibody	53-6.7	FC
Spark UV™ 387 anti-mouse CD8a	53-6.7	FC
Spark Blue™ 515 anti-mouse CD8a	53-6.7	FC
APC/Fire™ 810 anti-mouse CD8a	53-6.7	FC
Spark Red™ 718 anti-mouse CD8a (Flexi-Fluor™)	53-6.7	FC
PE/Fire™ 810 anti-mouse CD8a	53-6.7	FC
Spark PLUS UV395™ anti-mouse CD8a	53-6.7	FC
PerCP/Fire™ 780 anti-mouse CD8a	53-6.7	FC

Certificate of Analysis

Go To Top Version: 1 Revision Date: 04.18.2023

For Research Use Only. Not for diagnostic or therapeutic use.

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

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