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Spark Blue™ 550 anti-human CD335 (NKp46) (Flexi-Fluor™) Antibody

Pricing & Availability
Clone
9E2 (See other available formats)
Regulatory Status
RUO
Other Names
NKp46, NCR1
Isotype
Mouse IgG1, κ
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Product Citations
publications
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Cat # Size Price Quantity Save
285253 50 µg 316€
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Description

CD335, also known as NKp46, is a member of the natural cytotoxicity receptor (NCR) family which triggers cytotoxicity in NK cells. CD335 is direct­ly involved in target cell recognition and lysis, and is exclusively expressed on CD3-CD56+ NK cells, suggesting it is a universal marker for NK cells. NKp46, along with NKp30 and NKp44, is referred to as a natural cytoxicity receptor (NCR) and plays a very important role in killing virus-infected tumor cells and MHC-class I-unprotected cells.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
NKp46-Fc fusion protein
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation
The antibody was purified by affinity chromatography and conjugated with Spark Blue™ 550 under optimal conditions.
Concentration
0.2 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC
Recommended Usage

Flexi-Fluors™ are provided at a standard 0.2 mg/mL concentration. We recommend titrating this reagent to determine the optimal concentration for each application. For many flow cytometry applications, conjugated antibodies perform well at concentrations ranging from 0.03 to 1.0 µg per million cells in 100 µL. We recommend testing a range of concentrations starting from 10 µg/mL.

For example, make five 1:1 serial dilutions of the 0.2 mg/mL antibody. Add 5 µL of each dilution (including the undiluted antibody) to 100 µL of cells (at 107 cells/mL) to test six concentrations -- 1.0, 0.5, 0.25, 0.125, 0.06, and 0.03 µg per million cells in 100 µL volume. Compare staining patterns or create a titration curve using the MFI or staining index to determine the optimal concentration.

Excitation Laser
Blue Laser (488 nm)
Application Notes

Clone 9E2 has been shown to block NK activation through NKp46.6

Additional Product Notes

For more information about Flexi-Fluors™, visit our Flexi-Fluor™ page and review FAQs associated with this product line.

Application References

(PubMed link indicates BioLegend citation)
  1. Nakajima H, et al. 2000. Eur. J. Immunol. 30:3309.
  2. Kalberer CP, et al. 2003. Blood 102:127.
  3. Chen Y, et al. 2007. J. Immunol. 179:2766.
  4. Jarahian M, et al. 2009. J. Virol. 83:8108. PubMed
  5. Correia DV, et al. 2011. Blood 118:992. (FC) PubMed
  6. Achdout H. et al. 2010. J. Virol. 84:3993.
RRID
AB_3662557 (BioLegend Cat. No. 285253)

Antigen Details

Structure
Type I membrane glycoprotein (46 kD)
Distribution

Expressed on resting and activated NK cells

Cell Type
NK cells
Biology Area
Immunology, Innate Immunity
Molecular Family
CD Molecules
Antigen References

1. Mandelboim O and Porgador A. 2001. Int. J. Biochem. Cell Biol. 33:1147.
2. Nakajima H, et al. 2000. Eur. J. Immunol. 30:3309.
3. Sivori S. 1999. Eur. J. Immunol. 29:1656.

Gene ID
NA
UniProt
View information about CD335 on UniProt.org

Related FAQs

What are Flexi-Fluors?

Flexi-Fluors are rapidly made-to-order conjugated antibodies. The technology, manufacturing processes, and specifications used to create Flexi-Fluors are the same as our regular catalog products. However, the optimal concentration and performance of each Flexi-Fluor must be determined by the customer. 

How quickly will I receive my order?

We aim to ship Flexi-Fluors within 2-3 weeks of receipt of your order. However, depending on your location, shipping times may vary. 

How are Flexi-Fluors different from regular catalog products?

Flexi-Fluors are made on demand, specifically for you. Flexi-Fluors are manufactured using the same high-quality standards, and specifications as other catalog products. For faster delivery, Flexi-Fluors are not tested by flow cytometry to determine optimal concentrations or evaluate performance. This testing needs to be performed by the customer.

How do I determine the optimal concentration for using my Flexi-Fluor? How should I titrate my antibody?

Flexi-Fluors are provided at a standard 0.2 mg/mL concentration. We recommend that you titrate your antibody to determine the optimal concentration to use for your application. For many flow cytometry applications, conjugated antibodies perform well at concentrations ranging from 0.03 to 1.0 µg per million cells in 100 µL volume. We recommend that you test a range of concentrations starting from 10 µg/mL. 

 

For example, make five 1:1 serial dilutions of your 0.2 mg/mL antibody.  Add 5 µL of each dilution (including the undiluted antibody) to 100 µL of cells (at 107 cells/ml) to test six concentrations - 1.0, 0.5, 0.25, 0.125, 0.06, and 0.03 µg per million cells in 100 µL volume. Compare staining patterns or create a titration curve using the MFI or staining index to determine the optimal concentration. 

I can’t find the antibody-dye combination that I need. When will it be available?

We continuously update our catalog, introducing scores of new products every month. Please get in touch with our Technical Service team for an update on new products or recommendations for suitable alternatives to complete your panel. Or contact Custom Solutions to inquire about our affordable custom conjugation services. 

I need help to validate the performance of my Flexi-Fluor. Who should I contact?

Please get in touch with Technical Service for assistance. 

Can I order more than 50 μg of a Flexi-Fluor?

Yes, you can order multiple vials of the same Flexi-Fluor products.  We cannot guarantee, however, that these vials will be bottled from the same lot. For bulk single-lot orders, contact our Custom Solutions team.

What is the expiration date of my Flexi-Fluor?

Expiration dates can be found on the vial label or by using our CoA lookup tool. 

Other Formats

View All CD335 Reagents Request Custom Conjugation
Description Clone Applications
Purified anti-human CD335 (NKp46) 9E2 FC
Biotin anti-human CD335 (NKp46) 9E2 FC
PE anti-human CD335 (NKp46) 9E2 FC
Alexa Fluor® 647 anti-human CD335 (NKp46) 9E2 FC
Pacific Blue™ anti-human CD335 (NKp46) 9E2 FC
Brilliant Violet 421™ anti-human CD335 (NKp46) 9E2 FC
PerCP/Cyanine5.5 anti-human CD335 (NKp46) 9E2 FC
APC anti-human CD335 (NKp46) 9E2 FC
PE/Cyanine7 anti-human CD335 (NKp46) 9E2 FC
FITC anti-human CD335 (NKp46) 9E2 FC
Brilliant Violet 510™ anti-human CD335 (NKp46) 9E2 FC
Brilliant Violet 605™ anti-human CD335 (NKp46) 9E2 FC
Brilliant Violet 650™ anti-human CD335 (NKp46) 9E2 FC
PE/Dazzle™ 594 anti-human CD335 (NKp46) 9E2 FC
Alexa Fluor® 700 anti-human CD335 (NKp46) 9E2 FC
Brilliant Violet 711™ anti-human CD335 (NKp46) 9E2 FC
APC/Fire™ 750 anti-human CD335 (NKp46) 9E2 FC
Alexa Fluor® 488 anti-human CD335 (NKp46) 9E2 FC
TotalSeq™-B0101 anti-human CD335 (NKp46) 9E2 PG
TotalSeq™-C0101 anti-human CD335 (NKp46) 9E2 PG
TotalSeq™-A0101 anti-human CD335 (NKp46) 9E2 PG
Brilliant Violet 785™ anti-human CD335 (NKp46) 9E2 FC
Ultra-LEAF™ Purified anti-human CD335 (NKp46) 9E2 FC,FA
APC/Cyanine7 anti-human CD335 (NKp46) Antibody 9E2 FC
PE/Cyanine5 anti-human CD335 (NKp46) 9E2 FC
PE/Fire™ 810 anti-human CD335 (NKp46) 9E2 FC
TotalSeq™-D0101 anti-human CD335 (NKp46) 9E2 PG
PE/Cyanine7 anti-human CD335 9E2 FC
Spark Red™ 718 anti-human CD335 (NKp46) (Flexi-Fluor™) 9E2 FC
GMP PE/Cyanine7 anti-human CD335 (NKp46) 9E2 FC
Spark Blue™ 574 anti-human CD335 (NKp46) (Flexi-Fluor™) 9E2 FC
Spark Blue™ 550 anti-human CD335 (NKp46) (Flexi-Fluor™) 9E2 FC
Go To Top Version: 1    Revision Date: 09.12.2024

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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