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- Clone
- G025H7 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- CXCR3, G protein-coupled receptor 9 (GPR9), CKR-L2
- Isotype
- Mouse IgG1, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
Human CXCR3, also known as GPR9, is a chemokine receptor that binds CXCL9, CXCL10, and CXCL11. It is a 38 kD seven-pass transmembrane receptor coupled to G-protein. CXCR3 is highly expressed by T cells (Th1), natural killer cells (NK cells), dendritic cells, mast cells, alveolar macrophages, eosinophils, and human airway epithelial cells. CXCR3 is important for effector lymphocyte recruitment into inflamed tissue in various inflammatory and autoimmune diseases, such as chronically inflamed liver, Crohn's disease, rheumatoid arthritis, multiple sclerosis, and inflammatory skin diseases.
Product DetailsProduct Details
- Verified Reactivity
- Human, Cynomolgus, Rhesus
- Reported Reactivity
- African Green, Baboon
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- Human CXCR3 transfectants
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
- Preparation
- The antibody was purified by affinity chromatography and conjugated with Spark Red™ 718 under optimal conditions.
- Concentration
- 0.2 mg/mL
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
FC
- Recommended Usage
-
Flexi-Fluors™ are provided at a standard 0.2 mg/mL concentration. We recommend titrating this reagent to determine the optimal concentration for each application. For many flow cytometry applications, conjugated antibodies perform well at concentrations ranging from 0.03 to 1.0 µg per million cells in 100 µL. We recommend testing a range of concentrations starting from 10 µg/mL.
For example, make five 1:1 serial dilutions of the 0.2 mg/mL antibody. Add 5 µL of each dilution (including the undiluted antibody) to 100 µL of cells (at 107 cells/mL) to test six concentrations -- 1.0, 0.5, 0.25, 0.125, 0.06, and 0.03 µg per million cells in 100 µL volume. Compare staining patterns or create a titration curve using the MFI or staining index to determine the optimal concentration. - Excitation Laser
-
Red Laser (633 nm)
- Additional Product Notes
-
For more information about Flexi-Fluors™, visit our Flexi-Fluor™ page and review FAQs associated with this product line.
- RRID
-
AB_3106328 (BioLegend Cat. No. 285070)
Antigen Details
- Structure
- CXC-chemokine receptor, G protein-coupled receptor, seven-pass transmembrane receptor
- Distribution
-
T cell subset, NK cells, plasmacytoid dendritic cells, GM-CSF activated CD34+ hematopoietic progenitors, mast cells, alveolar macrophages, eosinophils, and airway epithelial cells
- Function
- Essential in T cell recruitment to sites of inflammation
- Ligand/Receptor
- CXCL9, CXCL10, and CXCL11
- Cell Type
- Dendritic cells, Eosinophils, Epithelial cells, Hematopoietic stem and progenitors, Macrophages, Mast cells, NK cells, T cells, Tregs
- Biology Area
- Cell Biology, Immunology, Neuroinflammation, Neuroscience
- Molecular Family
- CD Molecules, Cytokine/Chemokine Receptors, GPCR
- Antigen References
-
1. Loetscher M, et al. 1996. J. Exp. Med. 184:963.
2. Cole KE, et al. 1998. J. Exp. Med. 187:2009.
3. Aksoy MO, et al. 2006. Am. J. Physiol. Lung Cell Mol. Physiol. 290:L909.
4. Curbishley SM, et al. 2005. Am. J. Pathol. 167:887.
5. Turner JE, et al. 2007. Mini. Rev. Med. Chem. 7:1089.
6. Wenzel J, et al. 2008. J. Invest. Dermatol. 128:67. - Gene ID
- 2833 View all products for this Gene ID
- UniProt
- View information about CD183 on UniProt.org
Related Pages & Pathways
Pathways
Related FAQs
- Does staining at room temperature or even at 37°C help for checking chemokine receptors expression?
-
Due to continuous recycling of many chemokine receptors, it may be worthwhile to consider staining at room temperature or at 37°C if the staining at lower temperature (which can potentially reduce receptor turnover) is not optimal.
- What are Flexi-Fluors?
-
Flexi-Fluors are rapidly made-to-order conjugated antibodies. The technology, manufacturing processes, and specifications used to create Flexi-Fluors are the same as our regular catalog products. However, the optimal concentration and performance of each Flexi-Fluor must be determined by the customer.
- How quickly will I receive my order?
-
We aim to ship Flexi-Fluors within 2-3 weeks of receipt of your order. However, depending on your location, shipping times may vary.
- How are Flexi-Fluors different from regular catalog products?
-
Flexi-Fluors are made on demand, specifically for you. Flexi-Fluors are manufactured using the same high-quality standards, and specifications as other catalog products. For faster delivery, Flexi-Fluors are not tested by flow cytometry to determine optimal concentrations or evaluate performance. This testing needs to be performed by the customer.
- How do I determine the optimal concentration for using my Flexi-Fluor? How should I titrate my antibody?
-
Flexi-Fluors are provided at a standard 0.2 mg/mL concentration. We recommend that you titrate your antibody to determine the optimal concentration to use for your application. For many flow cytometry applications, conjugated antibodies perform well at concentrations ranging from 0.03 to 1.0 µg per million cells in 100 µL volume. We recommend that you test a range of concentrations starting from 10 µg/mL.
For example, make five 1:1 serial dilutions of your 0.2 mg/mL antibody. Add 5 µL of each dilution (including the undiluted antibody) to 100 µL of cells (at 107 cells/ml) to test six concentrations - 1.0, 0.5, 0.25, 0.125, 0.06, and 0.03 µg per million cells in 100 µL volume. Compare staining patterns or create a titration curve using the MFI or staining index to determine the optimal concentration.
- I can’t find the antibody-dye combination that I need. When will it be available?
-
We continuously update our catalog, introducing scores of new products every month. Please get in touch with our Technical Service team for an update on new products or recommendations for suitable alternatives to complete your panel. Or contact Custom Solutions to inquire about our affordable custom conjugation services.
- I need help to validate the performance of my Flexi-Fluor. Who should I contact?
-
Please get in touch with Technical Service for assistance.
- Can I order more than 50 μg of a Flexi-Fluor?
-
Yes, you can order multiple vials of the same Flexi-Fluor products. We cannot guarantee, however, that these vials will be bottled from the same lot. For bulk single-lot orders, contact our Custom Solutions team.
- What is the expiration date of my Flexi-Fluor?
-
Expiration dates can be found on the vial label or by using our CoA lookup tool.
Other Formats
View All CD183 Reagents Request Custom ConjugationCompare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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Purified anti-human CD183 (CXCR3)
Human peripheral lymphocytes were stained with purified anti... -
APC/Cyanine7 anti-human CD183 (CXCR3)
Human peripheral blood lymphocytes were stained with CD3 FIT... -
FITC anti-human CD183 (CXCR3)
Human peripheral lymphocytes were stained with CD3 PE and CX... 
-
PE anti-human CD183 (CXCR3)
Human peripheral blood lymphocytes were stained with CD3 APC... 
-
APC anti-human CD183 (CXCR3)
Human peripheral lymphocytes were stained with CD3 FITC and ... 
-
Alexa Fluor® 488 anti-human CD183 (CXCR3)
Human peripheral blood lymphocytes were stained with CD3 APC... 
-
Alexa Fluor® 647 anti-human CD183 (CXCR3)
Human peripheral blood lymphocytes were stained with CD3 PE ... 
-
PerCP/Cyanine5.5 anti-human CD183 (CXCR3)
Human peripheral lymphocytes were stained with CD3 APC and C... -
Brilliant Violet 421™ anti-human CD183 (CXCR3)
Human peripheral blood lymphocytes were stained with CD3 FIT... 
-
PE/Cyanine7 anti-human CD183 (CXCR3)
Human peripheral lymphocytes were stained with CD3 FITC and ... -
Pacific Blue™ anti-human CD183 (CXCR3)
-
Brilliant Violet 510™ anti-human CD183 (CXCR3)
Human peripheral blood lymphocytes were stained with CD3 APC... 
-
Brilliant Violet 605™ anti-human CD183 (CXCR3)
Human peripheral blood lymphocytes were stained with CD3 APC... 
-
Brilliant Violet 650™ anti-human CD183 (CXCR3)
-
Brilliant Violet 711™ anti-human CD183 (CXCR3)
Human peripheral blood lymphocytes were stained with CD3 FIT... 
-
Purified anti-human CD183 (CXCR3) (Maxpar® Ready)
Human PBMCs stained with 170Er-anti-CD3 (UCHT1) and 156Gd-an... -
PE/Dazzle™ 594 anti-human CD183 (CXCR3)
Human peripheral blood lymphocytes were stained with CD3 APC... 
-
PerCP anti-human CD183 (CXCR3)
Human peripheral blood lymphocytes were stained with CD3 APC... 
-
Brilliant Violet 785™ anti-human CD183 (CXCR3)
Human peripheral blood lymphocytes were stained with CD3 Per... -
Alexa Fluor® 700 anti-human CD183 (CXCR3)
Human peripheral blood lymphocytes were stained with CD3 FIT... 
-
Biotin anti-human CD183 (CXCR3)
Human peripheral blood lymphocytes were stained with CD3 FIT... 
-
TotalSeq™-A0140 anti-human CD183 (CXCR3)
-
TotalSeq™-C0140 anti-human CD183 (CXCR3)
-
Ultra-LEAF™ Purified anti-human CD183 (CXCR3)
Anti-human CXCR3 (G025H7) blocks the chemotaxis of Baf3-hCXC...
Human peripheral lymphocytes were stained with purified anti... -
TotalSeq™-B0140 anti-human CD183 (CXCR3)
-
APC/Fire™ 750 anti-human CD183 (CXCR3)
Human peripheral blood lymphocytes were stained with anti-hu... -
TotalSeq™-D0140 anti-human CD183 (CXCR3)
-
PE/Fire™ 810 anti-human CD183 (CXCR3) Antibody
Human peripheral blood lymphocytes were stained with anti-hu... -
APC/Fire™ 810 anti-human CD183 (CXCR3) Antibody
Human peripheral blood lymphocytes were stained with anti-hu... -
PE/Cyanine5 anti-human CD183 (CXCR3) Antibody
Human peripheral blood lymphocytes were stained with CD3 FIT... -
PE/Fire™ 640 anti-human CD183 (CXCR3)
Human peripheral lymphocytes were stained with anti-human C... -
PerCP/Fire™ 780 anti-human CD183 (CXCR3)
Human peripheral blood lymphocytes were stained with anti-hu... -
Spark Red™ 718 anti-human CD183 (CXCR3) (Flexi-Fluor™)
-
PE/Fire™ 700 anti-human CD183 (CXCR3)
Human peripheral blood lymphocytes were stained with anti-hu...
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