- Clone
- B3 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- T cell receptor Vγ9, TCR Vγ2 (Strauss, Quertermous nomenclature), TRGV9, TCRGV2S1
- Isotype
- Mouse IgG1, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
The Vγ9 TCR is a variant of the TCR γ chain expressed on a subset of γ/δ T cells. Vγ9Vδ2 T lymphocytes, a major γ/δ T cell subset in humans, recognize phosphoantigens, certain tumor cells, and cells treated with aminobisphosphonates. This cell population displays cytolytic activity against various tumor cells. The γ/δ TCR is a heterodimeric TCR complex composed of covalently bound γ and δ chains involved in antigen recognition and the non-covalently associated monomorphic proteins CD3δ, γ, ε, and ζ chains.
The B3 antibody reacts specifically with human TCR Vγ9 as designated by the Lefranc/Foster nomenclature system. Human TCR Vγ9 is also known as TCR Vγ2 under the Strauss, Quertermous nomenclature system.
Several TCR γ and δ chain nomenclature systems exist. In order to consolidate the various nomenclature systems, we have created charts for corresponding names across some of the most popular naming methods. Click here to see charts.
Product Details
- Verified Reactivity
- Human
- Reported Reactivity
- Chimpanzee, Cynomolgus
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
- Preparation
- The antibody was purified by affinity chromatography and conjugated with Spark Red™ 718 under optimal conditions.
- Concentration
- 0.2 mg/mL
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
FC
- Recommended Usage
-
Flexi-Fluors™ are provided at a standard 0.2 mg/mL concentration. We recommend titrating this reagent to determine the optimal concentration for each application. For many flow cytometry applications, conjugated antibodies perform well at concentrations ranging from 0.03 to 1.0 µg per million cells in 100 µL. We recommend testing a range of concentrations starting from 10 µg/mL.
For example, make five 1:1 serial dilutions of the 0.2 mg/mL antibody. Add 5 µL of each dilution (including the undiluted antibody) to 100 µL of cells (at 107 cells/mL) to test six concentrations -- 1.0, 0.5, 0.25, 0.125, 0.06, and 0.03 µg per million cells in 100 µL volume. Compare staining patterns or create a titration curve using the MFI or staining index to determine the optimal concentration. - Excitation Laser
-
Red Laser (633 nm)
- Additional Product Notes
-
For more information about Flexi-Fluors™, visit our Flexi-Fluor™ page and review FAQs associated with this product line.
-
Application References
(PubMed link indicates BioLegend citation) -
- Van Rhijn I, et al. 2003. Intl. Immunol. 15:373.
- Yoshino N, et al. 2000. Exp. Anim. (Tokyo) 49:97. (FC)
- RRID
-
AB_3662447 (BioLegend Cat. No. 285211)
Antigen Details
- Structure
- Ig superfamily member, TCR γ/δ with CD3 forms the CD3/TCR complex
- Distribution
-
Expressed on majority of peripheral γ/δ T cells
- Function
- Antigen recognition, T cell activation
- Ligand/Receptor
- Peptide bound to MHC
- Cell Type
- T cells
- Biology Area
- Adaptive Immunity, Immunology
- Molecular Family
- TCRs
- Antigen References
-
- Scotet E, et al. 2005. Immunity 22:71
- Rincon-Orozco B, et al. 2005. J. Immunol. 175:2144
- Gene ID
- NA
- UniProt
- View information about TCR Vgamma9 on UniProt.org
Related Pages & Pathways
Pages
Related FAQs
- What are Flexi-Fluors?
-
Flexi-Fluors are rapidly made-to-order conjugated antibodies. The technology, manufacturing processes, and specifications used to create Flexi-Fluors are the same as our regular catalog products. However, the optimal concentration and performance of each Flexi-Fluor must be determined by the customer.
- How quickly will I receive my order?
-
We aim to ship Flexi-Fluors within 2-3 weeks of receipt of your order. However, depending on your location, shipping times may vary.
- How are Flexi-Fluors different from regular catalog products?
-
Flexi-Fluors are made on demand, specifically for you. Flexi-Fluors are manufactured using the same high-quality standards, and specifications as other catalog products. For faster delivery, Flexi-Fluors are not tested by flow cytometry to determine optimal concentrations or evaluate performance. This testing needs to be performed by the customer.
- How do I determine the optimal concentration for using my Flexi-Fluor? How should I titrate my antibody?
-
Flexi-Fluors are provided at a standard 0.2 mg/mL concentration. We recommend that you titrate your antibody to determine the optimal concentration to use for your application. For many flow cytometry applications, conjugated antibodies perform well at concentrations ranging from 0.03 to 1.0 µg per million cells in 100 µL volume. We recommend that you test a range of concentrations starting from 10 µg/mL.
For example, make five 1:1 serial dilutions of your 0.2 mg/mL antibody. Add 5 µL of each dilution (including the undiluted antibody) to 100 µL of cells (at 107 cells/ml) to test six concentrations - 1.0, 0.5, 0.25, 0.125, 0.06, and 0.03 µg per million cells in 100 µL volume. Compare staining patterns or create a titration curve using the MFI or staining index to determine the optimal concentration.
- I can’t find the antibody-dye combination that I need. When will it be available?
-
We continuously update our catalog, introducing scores of new products every month. Please get in touch with our Technical Service team for an update on new products or recommendations for suitable alternatives to complete your panel. Or contact Custom Solutions to inquire about our affordable custom conjugation services.
- I need help to validate the performance of my Flexi-Fluor. Who should I contact?
-
Please get in touch with Technical Service for assistance.
- Can I order more than 50 μg of a Flexi-Fluor?
-
Yes, you can order multiple vials of the same Flexi-Fluor products. We cannot guarantee, however, that these vials will be bottled from the same lot. For bulk single-lot orders, contact our Custom Solutions team.
- What is the expiration date of my Flexi-Fluor?
-
Expiration dates can be found on the vial label or by using our CoA lookup tool.
Other Formats
View All TCR Vγ9 Reagents Request Custom ConjugationDescription | Clone | Applications |
---|---|---|
Purified anti-human TCR Vγ9 | B3 | FC |
FITC anti-human TCR Vγ9 | B3 | FC |
PE anti-human TCR Vγ9 | B3 | FC |
APC anti-human TCR Vγ9 | B3 | FC |
TotalSeq™-A0583 anti-human TCR Vγ9 | B3 | PG |
PE/Cyanine5 anti-human TCR Vγ9 | B3 | FC |
Alexa Fluor® 700 anti-human TCR Vγ9 | B3 | FC |
APC/Fire™ 750 anti-human TCR Vγ9 | B3 | FC |
TotalSeq™-C0583 anti-human TCR Vγ9 | B3 | PG |
PE/Cyanine7 anti-human TCR Vγ9 | B3 | FC |
TotalSeq™-B0583 anti-human TCR Vγ9 | B3 | PG |
PerCP/Cyanine5.5 anti-human TCR Vγ9 | B3 | FC |
APC/Cyanine7 anti-human TCR Vγ9 | B3 | FC |
Spark Red™ 718 anti-human TCR Vγ9 (Flexi-Fluor™) | B3 | FC |
Spark Blue™ 574 anti-human TCR Vγ9 (Flexi-Fluor™) | B3 | FC |
Spark Blue™ 550 anti-human TCR Vγ9 (Flexi-Fluor™) | B3 | FC |
Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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Purified anti-human TCR Vγ9
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FITC anti-human TCR Vγ9
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PE anti-human TCR Vγ9
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APC anti-human TCR Vγ9
-
TotalSeq™-A0583 anti-human TCR Vγ9
-
PE/Cyanine5 anti-human TCR Vγ9
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Alexa Fluor® 700 anti-human TCR Vγ9
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APC/Fire™ 750 anti-human TCR Vγ9
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TotalSeq™-C0583 anti-human TCR Vγ9
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PE/Cyanine7 anti-human TCR Vγ9
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TotalSeq™-B0583 anti-human TCR Vγ9
-
PerCP/Cyanine5.5 anti-human TCR Vγ9
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APC/Cyanine7 anti-human TCR Vγ9
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Spark Red™ 718 anti-human TCR Vγ9 (Flexi-Fluor™)
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Spark Blue™ 574 anti-human TCR Vγ9 (Flexi-Fluor™)
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Spark Blue™ 550 anti-human TCR Vγ9 (Flexi-Fluor™)
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