Spark Violet™ 500 anti-human CD138 (Syndecan-1) Antibody, CD138, MI15

Pricing & Availability

Clone: MI15 (See other available formats)
Regulatory Status: RUO
Workshop: HCDM listed
Other Names: B-B4
Isotype: Mouse IgG1, κ
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Product Citations: publications

MI15_SparkViolet500_CD138_Antibody_011323 — Human myeloma cell line U266 was stained with anti-human CD138 (Syndecan-1) (clone MI15) Spark Violet™ 500 (filled histogram) or mouse IgG1, κ Spark Violet™ 500 isotype control (open histogram).

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356551

25 tests

133€

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356552

100 tests

295€

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Description

CD138, a member of the syndecan protein family, is a type I integral membrane heparin sulfate proteoglycan also known as Syndecan-1. Syndecan-1 participates in cell proliferation, cell migration, and cell-matrix adhesion via interaction with collagen, fibronectin, and other soluble molecules (such as FGF-basic). It is expressed on normal and malignant human plasma cells, pre-B cells, epithelial cells, and endothelial cells, but not on mature circulating B-lymphocytes. It is also expressed on some non-hematopoietic cells, including embryonic mesenchymal cells, vascular smooth muscle cells, endothelial cells, and neural cells.

Product Details

Technical Data Sheet (pdf)

Product Details

Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: A mixture of U266 and XG-1 human myeloma cell lines.
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation: The antibody was purified by affinity chromatography and conjugated with Spark Violet™ 500 under optimal conditions.
Concentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.

* Spark Violet™ 500 has a maximum excitation of 393 nm and a maximum emission of 500 nm.
Excitation Laser: Violet Laser (405 nm)
Application Notes: The epitope recognized by MI15 is found within the ectodomain of the CD138 core protein. It has been reported that MI15 blocks the binding of clone B-B4 but not clone DL-101 by flow cytometric analysis. Clones DL-101 and MI15 exhibit differential staining patterns on in vitro generated plasma cells and some CD138⁺ cell lines.⁴

Additional reported applications for the relevant formats include: immunofluorescent staining¹, Western blotting², immunohistochemical staining of formalin-fixed paraffin-embedded frozen tissue sections³, and spatial biology (IBEX)^5,6.
RRID: AB_2936694 (BioLegend Cat. No. 356551)
AB_2936694 (BioLegend Cat. No. 356552)

Antigen Details

Structure: 100-200 kD type I integral transmembrane glycoprotein
Distribution: Plasma cells, pre-B cells, epithelial cells, endothelial cells
Function: Adhesion, controls cell morphology, regulates cell growth
Ligand/Receptor: FGFb, collagen, fibronectin
Cell Type: B cells, Endothelial cells, Epithelial cells, Plasma cells
Biology Area: Cell Adhesion, Cell Biology, Cell Motility/Cytoskeleton/Structure, Immunology, Neuroscience, Synaptic Biology
Molecular Family: Adhesion Molecules, CD Molecules
Antigen References: 1. Sanderson RD, et al. 1992. Cell. Regul. 1:27.
2. Zola H, et al. 2007. Leukocyte and Stromal Cell Molecules: The CD Markers. Wiley-Liss A John Wiley & Sons Inc, Publication.
Gene ID: 6382 View all products for this Gene ID
UniProt: View information about CD138 on UniProt.org

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Cell Surface Flow Cytometry Staining Protocol

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Pathways

B cell Development and Activation

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Other Formats

View All CD138 Reagents Request Custom Conjugation

Description	Clone	Applications
PE anti-human CD138 (Syndecan-1)	MI15	FC,SB
Purified anti-human CD138 (Syndecan-1)	MI15	FC,ICC,WB,IHC-P,SB
APC anti-human CD138 (Syndecan-1)	MI15	FC
FITC anti-human CD138 (Syndecan-1)	MI15	FC,SB
PerCP/Cyanine5.5 anti-human CD138 (Syndecan-1)	MI15	FC
Alexa Fluor® 700 anti-human CD138 (Syndecan-1)	MI15	FC
PE/Cyanine7 anti-human CD138 (Syndecan-1)	MI15	FC
Brilliant Violet 421™ anti-human CD138 (Syndecan-1)	MI15	FC
Brilliant Violet 510™ anti-human CD138 (Syndecan-1)	MI15	FC
Brilliant Violet 605™ anti-human CD138 (Syndecan-1)	MI15	FC
Brilliant Violet 711™ anti-human CD138 (Syndecan-1)	MI15	FC
Alexa Fluor® 647 anti-human CD138 (Syndecan-1)	MI15	FC,SB
Alexa Fluor® 594 anti-human CD138 (Syndecan-1)	MI15	ICC
PE/Dazzle™ 594 anti-human CD138 (Syndecan-1)	MI15	FC
APC/Cyanine7 anti-human CD138 (Syndecan-1)	MI15	FC
Pacific Blue™ anti-human CD138 (Syndecan-1)	MI15	FC
TotalSeq™-A0055 anti-human CD138 (Syndecan-1)	MI15	PG
Brilliant Violet 785™ anti-human CD138 (Syndecan-1)	MI15	FC
Biotin anti-human CD138 (Syndecan-1)	MI15	FC
TotalSeq™-C0055 anti-human CD138 (Syndecan-1)	MI15	PG
APC/Fire™ 750 anti-human CD138 (Syndecan-1)	MI15	FC
TotalSeq™-B0055 anti-human CD138 (Syndecan-1)	MI15	PG
PE/Cyanine5 anti-human CD138 (Syndecan-1)	MI15	FC
TotalSeq™-D0055 anti-human CD138 (Syndecan-1)	MI15	PG
PE/Fire™ 640 anti-human CD138 (Syndecan-1)	MI15	FC
Spark Violet™ 500 anti-human CD138 (Syndecan-1)	MI15	FC
FITC anti-human CD138	MI15	FC
PE/Fire™ 700 anti-human CD138 (Syndecan-1)	MI15	FC
Spark Violet™ 423 anti-human CD138 (Syndecan-1)	MI15	FC
Spark Red™ 718 anti-human CD138 (Syndecan-1) (Flexi-Fluor™)	MI15	FC
GMP FITC anti-human CD138 (Syndecan-1)	MI15	FC
Brilliant Violet 650™ anti-human CD138 (Syndecan-1)	MI15	FC

Certificate of Analysis

Go To Top Version: 1 Revision Date: 12.07.2022

For Research Use Only. Not for diagnostic or therapeutic use.

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

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