StarBright UltraViolet 795 anti-human CD185 (CXCR5) Antibody, CD185, J252D4

Pricing & Availability

Clone: J252D4 (See other available formats)
Regulatory Status: RUO
Other Names: BLR1, MDR15
Isotype: Mouse IgG1, κ
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Product Citations: publications

J252D4_StarBrightUV795_CD185_FC_1_042325 — Human peripheral blood lymphocytes were stained with anti-human CD19 (clone HIB19) FITC and anti-human CD185 (CXCR5) (clone J252D4) StarBright UltraViolet 795 (left) or stained with anti-human CD19 (clone HIB19) FITC only (right).

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356970

100 tests

300€

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This StarBright conjugate is available for purchase as part of a multicolor flow cytometry panel. To order, add compatible products to your shopping cart, or use our Panel Selector tool to build your ideal panel. For StarBright ordering assistance, contact your local field representative.

This product is also available for direct purchase within a StarBright Basic Panel, which combines a minimal set of pre-selected compatible reagents.

Description

CD185, also known as CXCR5, is a 42 kD G-protein coupled receptor with seven transmembrane regions. CXCR5 is expressed by mature B cells, follicular helper T cells, Burkitt’s lymphoma cells and a subset of neurons, and mediates cell migration to the B cell follicles in the secondary lymphoid organs. The ligand of CXCR5 is CXCL13 (BLC).

Product Details

Technical Data Sheet (pdf)

Product Details

Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Human CXCR5-transfected cells
Formulation: Phosphate-buffered saline, containing 0.09% sodium azide, 0.05% Tween 20, 0.1% PEG 3350, 0.1% Pluronic F68, and 1% BSA (origin USA)
Preparation: The antibody was purified by affinity chromatography and conjugated with StarBright UltraViolet 795 under optimal conditions.
Concentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.) The concentration relates to the dye component of the conjugate.
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.

* StarBright UltraViolet 795 has a maximum excitation of 340 nm and a maximum emission of 792 nm.

StarBright dyes are powered by Bio-Rad®. STARBRIGHT is a trademark of Bio-Rad Laboratories, Inc.
Excitation Laser: Ultraviolet Laser (355 nm)

Antigen Details

Structure: G-protein coupled receptor, 7 transmembrane regions, 42 kD
Distribution: Mature B cells, Follicular helper T cells, and Burkitt's lymphoma cells.
Function: Mediates cell migration to the B cell follicles in the secondary lymphoid organs
Ligand/Receptor: CXCL13
Cell Type: B cells, Tfh
Biology Area: Cell Biology, Immunology, Neuroinflammation, Neuroscience
Molecular Family: CD Molecules, Cytokine/Chemokine Receptors, GPCR
Antigen References: 1. Ma CS, et al. 2012. J. Exp. Med. 209:1241.
2. León B, et al. 2012. Nat. Immunol. 13:681.
3. Crotty S. 2011. Annu. Rev. Immunol. 29:621.
4. Kerfoot SM, et al. 2011. Immunity 34:947.
5. Sáez de Guinoa J, et al. 2011. Blood 118:1560.
Gene ID: 643 View all products for this Gene ID
UniProt: View information about CD185 on UniProt.org

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Related Protocols

Cell Surface Flow Cytometry Staining Protocol

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Related Pages & Pathways

Pathways

Related FAQs

Does staining at room temperature or even at 37°C help for checking chemokine receptors expression?: Due to continuous recycling of many chemokine receptors, it may be worthwhile to consider staining at room temperature or at 37°C if the staining at lower temperature (which can potentially reduce receptor turnover) is not optimal.
What is a “StarBright panel”?: A StarBright panel is a multiplex panel that includes at least one StarBright conjugate.
Will my StarBright panel be provided as a cocktail?: StarBright products purchased as part of a panel or StarBright Basic Panel will be provided in individual vials. To order premade cocktails, please contact our Custom Solutions Team.
Are StarBright GMP/ASR products available?: StarBright ASR and GMP products are coming soon.
Are StarBright custom products available?: Yes, please contact our Custom Solutions Team.
Will StarBright isotype controls be offered?: We are not planning to offer StarBright isotype controls at this time. Fluorescence Minus One (FMO) controls should be used to define gating boundaries.
What amount of a StarBright conjugate should I use?: Antibody conjugates should be titrated for optimal performance in each application. To directly compare performance with a traditional or polymer-conjugated antibody, we recommend using the same volume of each product for analysis. Due to differences in the quantification of product mass, StarBright Dyes are prepared in equivalent volumes.
Why shouldn’t I compare the mass (μg) of a StarBright conjugate to a traditional or polymer conjugate?: For StarBright conjugates, the reported mass refers to the mass of the dye component. For traditional conjugates, the reported mass refers to the mass of the antibody component. This is because traditional conjugates have multiple dye molecules attached to one antibody, while StarBright conjugates have multiple antibodies attached to a single StarBright Dye.

Other Formats

View All CD185 Reagents Request Custom Conjugation

Description	Clone	Applications
PE anti-human CD185 (CXCR5)	J252D4	FC
APC anti-human CD185 (CXCR5)	J252D4	FC
Purified anti-human CD185 (CXCR5)	J252D4	FC
Alexa Fluor® 647 anti-human CD185 (CXCR5)	J252D4	FC
PerCP/Cyanine5.5 anti-human CD185 (CXCR5)	J252D4	FC
Alexa Fluor® 488 anti-human CD185 (CXCR5)	J252D4	FC
FITC anti-human CD185 (CXCR5)	J252D4	FC
Alexa Fluor® 700 anti-human CD185 (CXCR5)	J252D4	FC
Pacific Blue™ anti-human CD185 (CXCR5)	J252D4	FC
Brilliant Violet 421™ anti-human CD185 (CXCR5)	J252D4	FC
PE/Cyanine7 anti-human CD185 (CXCR5)	J252D4	FC
APC/Cyanine7 anti-human CD185 (CXCR5)	J252D4	FC
PE/Dazzle™ 594 anti-human CD185 (CXCR5)	J252D4	FC
Brilliant Violet 605™ anti-human CD185 (CXCR5)	J252D4	FC
Biotin anti-human CD185 (CXCR5)	J252D4	FC
Brilliant Violet 711™ anti-human CD185 (CXCR5)	J252D4	FC
Brilliant Violet 785™ anti-human CD185 (CXCR5)	J252D4	FC
TotalSeq™-A0144 anti-human CD185 (CXCR5)	J252D4	PG
TotalSeq™-C0144 anti-human CD185 (CXCR5)	J252D4	PG
Brilliant Violet 750™ anti-human CD185 (CXCR5)	J252D4	FC
TotalSeq™-B0144 anti-human CD185 (CXCR5)	J252D4	PG
APC/Fire™ 750 anti-human CD185 (CXCR5)	J252D4	FC
Spark NIR™ 685 anti-human CD185 (CXCR5)	J252D4	FC
TotalSeq™-D0144 anti-human CD185 (CXCR5)	J252D4	PG
PE/Cyanine5 anti-human CD185 (CXCR5)	J252D4	FC
PE/Fire™ 700 anti-human CD185 (CXCR5) Antibody	J252D4	FC
APC/Fire™ 810 anti-human CD185 (CXCR5) Antibody	J252D4	FC
PerCP/Fire™ 806 anti-human CD185 (CXCR5)	J252D4	FC
PerCP/Fire™ 780 anti-human CD185 (CXCR5)	J252D4	FC
Spark Red™ 718 anti-human CD185 (CXCR5)	J252D4	FC
APC anti-human CD185	J252D4	FC
Brilliant Violet 510™ anti-human CD185 (CXCR5)	J252D4	FC
Brilliant Violet 650™ anti-human CD185 (CXCR5)	J252D4	FC
PE anti-human CD185	J252D4	FC
PE/Fire™ 744 anti-human CD185 (CXCR5) Antibody	J252D4	FC
StarBright UltraViolet 795 anti-human CD185 (CXCR5)	J252D4	FC

Certificate of Analysis

Go To Top Version: 1 Revision Date: 04.15.2025

For Research Use Only. Not for diagnostic or therapeutic use.

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.