TotalSeq™-D0158 anti-human CD134 (OX40) Antibody, CD134, Ber-ACT35 (ACT35)

Pricing & Availability

Clone: Ber-ACT35 (ACT35) (See other available formats)
Regulatory Status: RUO
Other Names: ACT35 antigen, tumor necrosis factor receptor superfamily member 4 (TNFRSF4)
Isotype: Mouse IgG1, κ
Barcode Sequence: AACCCACCGTTGTTA
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Product Citations: publications

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350039

10 µg

296€

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Description

CD134, also known as OX40 and TNFRSF4, is a 50 kD type I transmembrane glycoprotein. It is a member of the TNF receptor family. OX40 is expressed on activated T lymphocytes including Th1, Th2, Th17, and Treg cells. The interaction of OX40 with OX40L results in B cell proliferation and antibody secretion, regulation of primary T cell expansion, and T cell survival. OX40 influences the size of the T cell memory pool and regulation of CD4⁺ T cell tolerance.

Product Details

Technical Data Sheet (pdf)

Product Details

Verified Reactivity: Human
Reported Reactivity: Chimpanzee
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: HTLV 1-transformed HUT 102 cells
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and EDTA
Preparation: The antibody was purified by chromatography and conjugated with TotalSeq™-D oligomer under optimal conditions.
Concentration: 0.5 mg/mL
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C. Do not freeze.
Application: PG - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis and the oligomer sequence is confirmed by sequencing. TotalSeq™-D antibodies are compatible with Mission Bio’s Tapestri Single-Cell Sequencing Platform for simultaneous detection of DNA and Protein.

To maximize performance, it is strongly recommended that the reagent be titrated for each application, and that you centrifuge the antibody dilution before adding to the cells at 14,000xg at 2 - 8°C for 10 minutes. Carefully pipette out the liquid avoiding the bottom of the tube and add to the cell suspension. For Proteogenomics analysis, the suggested starting amount of this reagent for titration is ≤ 1.0 µg per million cells in 100 µL volume. Refer to the corresponding TotalSeq™ protocol for specific staining instructions.

Buyer is solely responsible for determining whether Buyer has all intellectual property rights that are necessary for Buyer's intended uses of the BioLegend TotalSeq™ products. For example, for any technology platform Buyer uses with TotalSeq™, it is Buyer's sole responsibility to determine whether it has all necessary third party intellectual property rights to use that platform and TotalSeq™ with that platform.
Application Notes: Additional reported applications (for the relevant formats) include: Western blotting¹, immunoprecipitation¹, immunohistochemical staining^2,3of paraffin embedded⁷ and frozen tissue sections, ELISA⁴, and functional assay⁵. The LEAF™ or Ultra-LEAF™ purified antibody is recommended for functional assays (contact our custom solutions team).
Additional Product Notes: TotalSeq™-D reagents are designed to profile protein expression at single cell level. The Mission Bio Tapestri platform and sequencer (e.g. Illumina analyzers) are required. Please contact technical support for more information, or visit biolegend.com/totalseq/single-cell-dna

The barcode flanking sequences are CGAGATGACTACGCTACTCATGG (PCR handle), and GAGCCGATCTAGTATCTCAGT*C*G (capture sequence). * indicates a phosphorothioated bond, to prevent nuclease degradation.

View more applications data for this product in our Application Technical Notes.
RRID: AB_2892425 (BioLegend Cat. No. 350039)

Antigen Details

Structure: 50 kD, TNF receptor family
Distribution: Activated T cells
Function: Receptor for OX40 ligand, co-stimulatory signal for T cell proliferation and survival.
Ligand/Receptor: OX40L (CD252)
Cell Type: T cells, Tregs
Biology Area: Apoptosis/Tumor Suppressors/Cell Death, Cell Biology, Costimulatory Molecules, Immunology
Molecular Family: CD Molecules, Immune Checkpoint Receptors
Antigen References: 1. Smith CA, et al. 1994. Cell. 76:959.
2. Chen AL, et al. 1999. Immunity. 11:689.
3. Croft M. 2010. Annu. Rev. Immunol. 28:57.
4. Withers DR, et al. 2009. J. Immunol. 183:5079.
5. Klinger M, et al. 2009. J. Immunol. 182:4581.
Gene ID: 7293 View all products for this Gene ID
UniProt: View information about CD134 on UniProt.org

Documentation

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Related Protocols

TotalSeq™-D with Mission Bio Tapestri® Single-Cell DNA + Protein Protocol

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Related Pages & Pathways

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Other Formats

View All CD134 Reagents Request Custom Conjugation

Description	Clone	Applications
FITC anti-human CD134 (OX40)	Ber-ACT35 (ACT35)	FC
Purified anti-human CD134 (OX40)	Ber-ACT35 (ACT35)	FC,IHC-P,ELISA,IHC-F,WB
PE anti-human CD134 (OX40)	Ber-ACT35 (ACT35)	FC
APC anti-human CD134 (OX40)	Ber-ACT35 (ACT35)	FC
PerCP/Cyanine5.5 anti-human CD134 (OX40)	Ber-ACT35 (ACT35)	FC
PE/Cyanine7 anti-human CD134 (OX40)	Ber-ACT35 (ACT35)	FC
Brilliant Violet 421™ anti-human CD134 (OX40)	Ber-ACT35 (ACT35)	FC
Purified anti-human CD134 (OX40) (Maxpar® Ready)	Ber-ACT35 (ACT35)	FC,CyTOF®
Alexa Fluor® 647 anti-human CD134 (OX40)	Ber-ACT35 (ACT35)	FC
PE/Dazzle™ 594 anti-human CD134 (OX40)	Ber-ACT35 (ACT35)	FC
APC/Cyanine7 anti-human CD134 (OX40)	Ber-ACT35 (ACT35)	FC
Biotin anti-human CD134 (OX40)	Ber-ACT35 (ACT35)	FC
Brilliant Violet 510™ anti-human CD134 (OX40)	Ber-ACT35 (ACT35)	FC
Brilliant Violet 605™ anti-human CD134 (OX40)	Ber-ACT35 (ACT35)	FC
APC/Fire™ 750 anti-human CD134 (OX40)	Ber-ACT35 (ACT35)	FC
Brilliant Violet 711™ anti-human CD134 (OX40)	Ber-ACT35 (ACT35)	FC
TotalSeq™-A0158 anti-human CD134 (OX40)	Ber-ACT35 (ACT35)	PG
TotalSeq™-C0158 anti-human CD134 (OX40)	Ber-ACT35 (ACT35)	PG
TotalSeq™-B0158 anti-human CD134 (OX40)	Ber-ACT35 (ACT35)	PG
TotalSeq™-D0158 anti-human CD134 (OX40)	Ber-ACT35 (ACT35)	PG
PE/Cyanine5 anti-human CD134 (OX40)	Ber-ACT35 (ACT35)	FC
PE/Fire™ 810 anti-human CD134 (OX40)	Ber-ACT35 (ACT35)	FC
PE anti-human CD134	Ber-ACT35 (ACT35)	FC
PE/Fire™ 744 anti-human CD134 (OX40)	Ber-ACT35 (ACT35)	FC
Brilliant Violet 785™ anti-human CD134 (OX40)	Ber-ACT35 (ACT35)	FC
Brilliant Violet 650™ anti-human CD134 (OX40)	Ber-ACT35 (ACT35)	FC

Certificate of Analysis

Go To Top Version: 1 Revision Date: 05.20.2021

For Research Use Only. Not for diagnostic or therapeutic use.

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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