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Ultra-LEAF™ Purified anti-human CD114 (G-CSFR) Antibody

Pricing & Availability
Clone
W20249H (See other available formats)
Regulatory Status
RUO
Other Names
Colony Stimulating Factor 3 Receptor (CSF3R), Granulocyte Colony-Stimulating Factor Receptor (GCSFR), CD114, CD114 Antigen, G-CSF-R, SCN7
Isotype
Rat IgG2b, κ
Ave. Rating
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Product Citations
publications
W20249H_ULEAF_CD114_Antibody_1_013124
Recombinant human G-CSF Receptor/CD114 (Cat. No. 769902) inhibits M-NFS-60 cell proliferation induced by human G-CSF (Cat. No. 578604) (black circles) at 0.4 ng/mL. Ultra- LEAF™ purified anti-human CD114 (G-CSFR) (clone W20249H) (purple squares) neutralizes the inhibitory effect of recombinant human G-CSF Receptor/CD114 induced by human G-CSF in M-NFS-60 cells in a dose-dependent manner, whereas GoInVivo™ purified Rat IgG2b, κ isotype control (Cat. No. 400666) (gray triangles) does not have effect. ND50 range: 0.04 - 0.3 µg/mL.
  • W20249H_ULEAF_CD114_Antibody_1_013124
    Recombinant human G-CSF Receptor/CD114 (Cat. No. 769902) inhibits M-NFS-60 cell proliferation induced by human G-CSF (Cat. No. 578604) (black circles) at 0.4 ng/mL. Ultra- LEAF™ purified anti-human CD114 (G-CSFR) (clone W20249H) (purple squares) neutralizes the inhibitory effect of recombinant human G-CSF Receptor/CD114 induced by human G-CSF in M-NFS-60 cells in a dose-dependent manner, whereas GoInVivo™ purified Rat IgG2b, κ isotype control (Cat. No. 400666) (gray triangles) does not have effect. ND50 range: 0.04 - 0.3 µg/mL.
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629653 100 µg 288€
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Description

Human G-CSF receptor (CSF3R) was initially isolated from the cDNA libraries of human U937 leukemia cells. The human G-CSF receptor is a type I cytokine receptor, possesses 836 amino acids, and has a clear homology (62.5%) with its murine counterpart. G-CSFR contains a conserved cytokine receptor homologous (CRH) domain, an Ig-like domain and three fibronectin type III-like domains in the extracellular region; a transmembrane region; and an intracellular region lacking intrinsic catalytic activity. Human G-CSF soluble receptor has been identified this form has a deletion of the transmembrane domain. The complex G-CSF/G-CSFR has a 2:2 ligand:receptor stoichiometry. G-CSFR stimulates signals transduction proteins that induce granulocytic proliferation and differentiation such as the STAT 1, 3 and 5 transcription factors, the Ras/Mek/Erk1/2 pathway, the Src-related kinases Lyn and Hck, the serine/threonine kinase Akt, and the Syk tyrosine kinas. Also, G-CSFR is associated to pathways that negatively regulate granulopoiesis, including those mediated by the SHP-1 tyrosine phosphatase and the suppressor of cytokine signaling 3 (SOCS3) protein. Different mutations in the gene encoding the G-CSFR have been described with clinical consequences related to the myeloid lineage, including severe congenital neutropenia (SCN), myelodysplastic syndrome (MDS), acute myeloid leukemia (AML), and chronic neutrophilic leukemia (CNL).

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Recombinant Human G-CSF Receptor/CD114
Formulation
0.2 µm filtered in phosphate-buffered solution, pH 7.2, containing no preservative.
Endotoxin Level
Less than 0.01 EU/µg of the protein (< 0.001 ng/µg of the protein) as determined by the LAL test.
Preparation
The Ultra-LEAF™ (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.
Concentration
The antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL. To obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF™ solution contains no preservative; handle under aseptic conditions.
Application

Neut - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by inhibition effect of recombinant human G-CSF Receptor/CD114 (Cat. No. 769902) at 0.1 µg/mL induced by human G-CSF (Cat. No. 578604) at 0.4 ng/mL in M-NFS-60 cells. ND50 range: 0.04 - 0.3 µg/mL. It is recommended that the reagent be titrated for optimal performance for each application.

RRID
AB_3097542 (BioLegend Cat. No. 629653)

Antigen Details

Structure
Cytokine receptor
Distribution

Neutrophils, hematopoietic progenitor cells, placenta neurons, adult neural stem cells, endothelial cells, and cardiomyocytes

Function
Controls production, differentiation, and function of granulocytes. Integrin α9β1 augments receptor signaling and granulopoiesis in vivo.
Interaction
G-CSFR interacts with integrin α9β1.
Ligand/Receptor
G-CSF
Cell Type
Embryonic Stem Cells, Hematopoietic stem and progenitors
Biology Area
Stem Cells
Molecular Family
Cytokine/Chemokine Receptors, Soluble Receptors
Antigen References
  1. Fukunaga R, et al. 1990. Proc Natl Acad Sci USA. 87:8702-6.
  2. Larsen A, et al. 1990. J Exp Med. 172:1559-70.
  3. Tamada T, et al. 2006. Proc Natl Acad Sci USA. 103:3135-40.
  4. Chen C, et al. 2006. Immunity. 25:895-906.
  5. Panopoulos AD & Watowich SS. 2008. Cytokine. 42:277-88.
  6. Liongue C & Ward AC. 2014. Front Oncol. 4:93.
Gene ID
1441 View all products for this Gene ID
UniProt
View information about CD114 on UniProt.org

Related FAQs

Do you guarantee that your antibodies are totally pathogen free?

BioLegend does not test for pathogens in-house aside from the GoInVivo™ product line. However, upon request, this can be tested on a custom basis with an outside, independent laboratory.

Does BioLegend test each Ultra-LEAF™ antibody by functional assay?

No, BioLegend does not test Ultra-LEAF™ antibodies by functional assays unless otherwise indicated. Due to the possible complexities and variations of uses of biofunctional antibodies in different assays and because of the large product portfolio, BioLegend does not currently perform functional assays as a routine QC for the antibodies. However, we do provide references in which the antibodies were used for functional assays and we do perform QC to verify the specificity and quality of the antibody based on our strict specification criteria.

Does BioLegend test each Ultra-LEAF™ antibody for potential pathogens?

No, BioLegend does not test for pathogens in-house unless otherwise indicated.  However, we can recommend an outside vendor to perform this testing as needed.

Have you tested this Ultra-LEAF™ antibody for in vivo or in vitro applications?

We don't test our antibodies for in vivo or in vitro applications unless otherwise indicated. Depending on the product, the TDS may describe literature supporting usage of a particular product for bioassay. It may be best to further consult the literature to find clone specific information.

Go To Top Version: 1    Revision Date: 01.31.2024

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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