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- Clone
- W20199I (See other available formats)
- Regulatory Status
- RUO
- Other Names
- CD40L, TNFSF5, T-BAM, gp39
- Isotype
- Rat IgG2b, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
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Human peripheral blood mononuclear cells were treated with Cell Activation Cocktail without Brefeldin A (Cat. No. 423302) for 6 hours. Cells were surface stained with Ultra-LEAF™ purified anti-human CD154 (clone W20199I) (left) or Ultra-LEAF™ purified rat IgG2b, κ isotype control, followed by goat anti-rat IgG PE. Cells were then co-stained with anti-human CD4 (clone RPA-T4) APC.
| Cat # | Size | Price | Quantity Check Availability | Save | ||
|---|---|---|---|---|---|---|
| 388205 | 100 µg | 376€ | ||||
CD154, also known as CD40L, TNFSF5, T-BAM, and gp39, is a 32-39 kD type II transmembrane glycoprotein member of the TNF superfamily expressed on activated T cells. CD154 interacts as a costimulatory molecule with CD40 on B Cells to induce B cell activation, resulting in proliferation, immunoglobulin production, and Ig Isotype switching. CD40L also may regulate T cell cytokine production.
Product DetailsProduct Details
- Verified Reactivity
- Human
- Antibody Type
- Monoclonal
- Host Species
- Rat
- Immunogen
- Recombinant human CD40L protein
- Formulation
- 0.2 µm filtered in phosphate-buffered solution, pH 7.2, containing no preservative.
- Endotoxin Level
- < 0.01 EU/μg of the protein (< 0.001 ng/μg of the protein) as determined by the LAL test
- Preparation
- The Ultra-LEAF™ (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.
- Concentration
- The antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL. To obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF™ solution contains no preservative; handle under aseptic conditions.
- Application
-
FC - Quality tested
- Recommended Usage
-
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.125 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
-
Clone W20199I is able to block the binding of clone 24-31 on target cells.
Clone W20199I is able to block the binding of CD40 and CD40L interaction on target cells.
Antigen Details
- Structure
- 32-39 kD type II transmembrane glycoprotein of the TNF superfamily
- Distribution
-
Activated T cells
- Function
- B cell costimulation
- Ligand/Receptor
- CD40
- Cell Type
- T cells, Tregs
- Biology Area
- Costimulatory Molecules, Immunology
- Molecular Family
- CD Molecules
- Antigen References
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- van Kooten C and Banchereau J. 2000. J Leukoc Biol. 67:2-17.
- Ford GS, et al. 1999. J Immunol. 162:4037-44.
- Gene ID
- 959 View all products for this Gene ID
- UniProt
- View information about CD154 on UniProt.org
Related Pages & Pathways
Pathways
Related FAQs
- Do you guarantee that your antibodies are totally pathogen free?
-
BioLegend does not test for pathogens in-house aside from the GoInVivo™ product line. However, upon request, this can be tested on a custom basis with an outside, independent laboratory.
- Does BioLegend test each Ultra-LEAF™ antibody by functional assay?
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No, BioLegend does not test Ultra-LEAF™ antibodies by functional assays unless otherwise indicated. Due to the possible complexities and variations of uses of biofunctional antibodies in different assays and because of the large product portfolio, BioLegend does not currently perform functional assays as a routine QC for the antibodies. However, we do provide references in which the antibodies were used for functional assays and we do perform QC to verify the specificity and quality of the antibody based on our strict specification criteria.
- Does BioLegend test each Ultra-LEAF™ antibody for potential pathogens?
-
No, BioLegend does not test for pathogens in-house unless otherwise indicated. However, we can recommend an outside vendor to perform this testing as needed.
- Have you tested this Ultra-LEAF™ antibody for in vivo or in vitro applications?
-
We don't test our antibodies for in vivo or in vitro applications unless otherwise indicated. Depending on the product, the TDS may describe literature supporting usage of a particular product for bioassay. It may be best to further consult the literature to find clone specific information.
Other Formats
View All CD154 Reagents Request Custom Conjugation| Description | Clone | Applications |
|---|---|---|
| PE anti-human CD154 | W20199I | FC |
Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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PE anti-human CD154
Human peripheral blood mononuclear cells were treated with C...
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