Ultra-LEAF™ Purified anti-human IL-12 (p70) Antibody

Product Details

Verified Reactivity

Human

Antibody Type

Monoclonal

Host Species

Rat

Formulation

0.2 µm filtered in phosphate-buffered solution, pH 7.2, containing no preservative.

Endotoxin Level

Less than 0.01 EU/µg of the protein (< 0.001 ng/µg of the protein) as determined by the LAL test.

Preparation

The Ultra-LEAF™ (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.

Concentration

The antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL. Older lots may have also been bottled at 1 mg/mL. To obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.

Storage & Handling

The antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF™ solution contains no preservative; handle under aseptic conditions.

Application

ELISA Capture, ELISPOT Capture

Recommended Usage

Each lot of this antibody is quality control tested by ELISA assay. For ELISA capture applications, a concentration range of 1 - 4 µg/mL is recommended. To obtain a linear standard curve, serial dilutions of IL-12 p70 recombinant protein ranging from 1000 to 8 pg/mL are recommended for each ELISA plate. For use as an ELISPOT capture antibody, a concentration range of 2 - 6 µg/mL is recommended. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

ELISA or ELISPOT capture: The purified 7B12 antibody is useful as a capture antibody for an IL-12 p70 specific sandwich ELISA or ELISPOT, when used in conjunction with Biotin anti-human IL-12/IL-23 p40 antibody (Cat. No. 508802/508805) as the detection antibody. The Ultra-LEAF™ Purified antibody is suggested for ELISPOT capture.
Note: For testing human IL-12/IL-23 p40 (monomer, dimer) in serum or plasma, BioLegend's ELISA Max™ Sets (Cat. No. 430701) are specially developed and recommended. For testing human IL-12 p70 in serum or plasma, BioLegend's ELISA Max™ Sets (Cat. No. 431701) are specially developed and recommended.

Application References

(PubMed link indicates BioLegend citation)

1. Shimomura Y, et al. 2008. J. Exp. Med. 205:1343. PubMed

RRID

AB_2861038 (BioLegend Cat. No. 511005)
AB_2861038 (BioLegend Cat. No. 511006)

Antigen Details

Structure

Cytokine; monomer, dimer (with p35 or p40)

Bioactivity

Induces IFN-γ, TNF-α production in T and NK cells; costimulation of PBL proliferation; proliferation/differentiation of T_H1 T lymphocytes

Cell Sources

Dendritic cells, monocytes/macrophages, B cells, T cells

Cell Targets

T cells, NK cells, PBL

Receptors

IL-12Rβ1 binds p40; dimeric with IL-12Rβ2 binds p35

Biology Area

Immunology

Molecular Family

Cytokines/Chemokines

Antigen References

1. Fitzgerald K, et al. Eds. 2001. The Cytokine FactsBook. Academic Press San Diego.
2. Quesniaux V. 1992. Research Immunol. 143:385.
3. Trinchieri G, et al. 1992 Prog. Growth Factor Res. 4:355.
4. Trinchieri G, et al. 1993 Immunol. Today. 14:335.
5. Oppmann B, et al. 2000 Immunity. 13:715.
6. Aggarwal S, et al. 2003 J. Biol. Chem.. 278:1910.
7. Parham C, et al. 2002 J. Immunol.. 168:5699.
8. Belladonna ML, et al. 2002 J. Immunol.. 168:5448.
9. Lankford CS, et al. 2003 J. Leukoc. Biol.. 73:49.

Regulation

Downregulated by IL-10; homodimeric p40 antagonistic to functional p70 heterodimer; p40 monomer has no function; p40 subunit in common with IL-23

Gene ID

3592 View all products for this Gene ID 3593 View all products for this Gene ID

UniProt

View information about IL-12 p70 on UniProt.org

Documentation

• Technical Data Sheet (pdf)
• Certificate of Analysis
• Safety Data Sheet

Reviews

Related Pages & Pathways

Related FAQs

Do you guarantee that your antibodies are totally pathogen free?

BioLegend does not test for pathogens in-house aside from the GoInVivo™ product line. However, upon request, this can be tested on a custom basis with an outside, independent laboratory.

Does BioLegend test each Ultra-LEAF™ antibody by functional assay?

No, BioLegend does not test Ultra-LEAF™ antibodies by functional assays unless otherwise indicated. Due to the possible complexities and variations of uses of biofunctional antibodies in different assays and because of the large product portfolio, BioLegend does not currently perform functional assays as a routine QC for the antibodies. However, we do provide references in which the antibodies were used for functional assays and we do perform QC to verify the specificity and quality of the antibody based on our strict specification criteria.

Does BioLegend test each Ultra-LEAF™ antibody for potential pathogens?

No, BioLegend does not test for pathogens in-house unless otherwise indicated.  However, we can recommend an outside vendor to perform this testing as needed.

Have you tested this Ultra-LEAF™ antibody for in vivo or in vitro applications?

We don't test our antibodies for in vivo or in vitro applications unless otherwise indicated. Depending on the product, the TDS may describe literature supporting usage of a particular product for bioassay. It may be best to further consult the literature to find clone specific information.

Other Formats

Certificate of Analysis