Ultra-LEAF™ Purified anti-human IL-4 Antibody

Product Details

Verified Reactivity

Human

Reported Reactivity

Pig, Rhesus

Antibody Type

Monoclonal

Host Species

Rat

Immunogen

CHO-expressed, recombinant human IL-4

Formulation

0.2 µm filtered in phosphate-buffered solution, pH 7.2, containing no preservative.

Endotoxin Level

Less than 0.01 EU/µg of the protein (< 0.001 ng/µg of the protein) as determined by the LAL test.

Preparation

The Ultra-LEAF™ (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.

Concentration

The antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL. Older lots may have also been bottled at 1 mg/mL. To obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.

Storage & Handling

The antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF™ solution contains no preservative; handle under aseptic conditions.

Application

ELISA Detection - Quality tested
CyTOF® - Verified
Neut, ICFC, IHC - Reported in the literature, not verified in house

Recommended Usage

Each lot of this antibody is quality control tested by ELISA assay.  Purified MP4-25D2 has been tested by blocking fluorochrome conjugated MP4-25D2 for intracellular cytokine staining. In order to obtain complete blocking results, a saturated amount of purified antibody (≤ 5.0 µg/million cells) should be used for incubation with target cells, prior to staining with fluorochrome conjugated antibody. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

ELISA Detection^1,3 or ELISPOT Detection^4,5: The biotinylated MP4-25D2 antibody is useful as a detection antibody for a sandwich ELISA or ELISPOT assay, when used in conjunction with purified 8D4-8 antibody (Cat. No. 500702/500707) as the capture antibody.
Flow Cytometry^6,9: The fluorochrome-labeled MP4-25D2 antibody is useful for intracellular immunofluorescent staining and flow cytometric analysis to identify IL-4 -producing cells within mixed cell populations.
Neutralization^1-3: The LEAF™ purified antibody (Endotoxin <0.1 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for neutralization of human IL-4 bioactivity (Cat. No. 500815). The MP4-25D2 antibody can neutralize the bioactivity of natural or recombinant IL-4.

Application References

(PubMed link indicates BioLegend citation)

1. Chretien I, et al. 1989. J. Immunol. Methods 117:67. (ELISA Detection, Neut)
2. Ramanathan L, et al. 1993. Biochem. 32:3549. (Neut)
3. Abrams J, et al. 1992. Immunol. Rev. 127:5. (ELISA Detection, Neut)
4. Mahanty S, et al. 1992. J. Immunol. 148:3567. (ELISPOT Detection)
5. Klinman D, et al. 1994. Curr. Prot. Immunol. John Wiley and Sons New York. Unit 6.19. (ELISPOT Detection)
6. Prussin C, et al. 1995. J. Immunol. Methods 188:117. (ICFC)
7. Raqib R, et al. 1995. Infect. Immun. 63:289.
8. Andersson J, et al. 1994. Immunology 83:16.
9. Iwamoto S, et al. 2007. J. Immunol. 179:1449. (ICFC) PubMed
10. Kubota M, et al. 1997. J. Immunol. 158:5321.
11. Dzhagalov I, et al. 2007. J. Immunol. 178:2113. PubMed
12. Kroneke MA, et al. 2012. J. Immunol. 188:3734. PubMed

Product Citations

1. Shen C, et al. 2021. Front Immunol. 12:680055. PubMed
2. Jiang J, et al. 2021. Front Immunol. 12:785549. PubMed

RRID

AB_2810615 (BioLegend Cat. No. 500841)
AB_2810615 (BioLegend Cat. No. 500840)
AB_2810615 (BioLegend Cat. No. 500842)
AB_2810615 (BioLegend Cat. No. 500837)
AB_2810615 (BioLegend Cat. No. 500838)
AB_2810615 (BioLegend Cat. No. 500839)

Antigen Details

Structure

Cytokine; 15-19 kD (Mammalian)

Bioactivity

Differentiation of naïve CD4⁺ T cells to the T_H2 type, proliferation/differentiation of activated B cells, expression of class II MHC antigens, and of low affinity IgE receptors in resting B cells

Cell Sources

Mast cells, T cells, bone marrow stromal cells

Cell Targets

B cells, T cells, monocytes, endothelial cells, fibroblasts

Receptors

Heterodimer IL-4Rα (CD124); γ-subunit (CD132) in common with IL-2R, IL-7R, IL-13R, IL-15R

Cell Type

Tregs

Biology Area

Cell Biology, Immunology, Neuroinflammation, Neuroscience

Molecular Family

Cytokines/Chemokines

Antigen References

1. Fitzgerald K, et al. Eds. 2001. The Cytokine FactsBook. Academic Press San Diego.
2. Boulay J, et al. 1992. Curr. Opin. Immunol. 4:294.
3. Dullens H, et al. 1991. In vivo 5:567.
4. Paul W. 1991. Blood 77:1859.

Regulation

Upregulated by IL-2, platelet activating factor; downregulated by TGF-β

Gene ID

3565 View all products for this Gene ID

UniProt

View information about IL-4 on UniProt.org

Documentation

• Technical Data Sheet (pdf)
• Certificate of Analysis
• Safety Data Sheet

Reviews

Related Pages & Pathways

Related FAQs

Do you guarantee that your antibodies are totally pathogen free?

BioLegend does not test for pathogens in-house aside from the GoInVivo™ product line. However, upon request, this can be tested on a custom basis with an outside, independent laboratory.

Does BioLegend test each Ultra-LEAF™ antibody by functional assay?

No, BioLegend does not test Ultra-LEAF™ antibodies by functional assays unless otherwise indicated. Due to the possible complexities and variations of uses of biofunctional antibodies in different assays and because of the large product portfolio, BioLegend does not currently perform functional assays as a routine QC for the antibodies. However, we do provide references in which the antibodies were used for functional assays and we do perform QC to verify the specificity and quality of the antibody based on our strict specification criteria.

Does BioLegend test each Ultra-LEAF™ antibody for potential pathogens?

No, BioLegend does not test for pathogens in-house unless otherwise indicated.  However, we can recommend an outside vendor to perform this testing as needed.

Have you tested this Ultra-LEAF™ antibody for in vivo or in vitro applications?

We don't test our antibodies for in vivo or in vitro applications unless otherwise indicated. Depending on the product, the TDS may describe literature supporting usage of a particular product for bioassay. It may be best to further consult the literature to find clone specific information.

Other Formats

Certificate of Analysis