Ultra-LEAF™ Purified anti-rat CD3 Antibody, CD3, 1F4

Pricing & Availability

Clone: 1F4 (See other available formats)
Regulatory Status: RUO
Other Names: T cell antigen receptor complex, T3
Isotype: Mouse IgM, κ
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Product Citations: publications

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201415

100 µg

216€

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201416

1 mg

542€

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Select size of product is eligible for a 40% discount! Promotion valid until December 31, 2024. Exclusions apply. To view full promotion terms and conditions or to contact your local BioLegend representative to receive a quote, visit our webpage.

Description

CD3 is a complex composed of δ, γ, ε, and ζ chains. They are 20-25 kD members of the immunoglobulin superfamily and associated with the T cell receptor (TCR). CD3 is expressed on thymocytes, peripheral T cells, some NK-T cells, and dendritic epidermal T cells. CD3 is involved in antigen recognition, signal transduction, and T cell activation.

Product Details

Technical Data Sheet (pdf)

Product Details

Verified Reactivity: Rat
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: F344 rat spleen cells stimulated with PMA and calcium ionophore
Formulation: 0.2 µm filtered in phosphate-buffered solution, pH 7.2, containing no preservative.
Endotoxin Level: Less than 0.01 EU/µg of the protein (< 0.001 ng/µg of the protein) as determined by the LAL test.
Preparation: The Ultra-LEAF™ (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.
Concentration: The antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL. Older lots may have also been bottled at 1 mg/mL. To obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF™ solution contains no preservative; handle under aseptic conditions.
Application: FC - Quality tested
IHC-F - Reported in the literature, not verified in house
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.
Application Notes: Immobilized 1F4 antibody can induce T cell proliferation in vitro. Additional reported applications (for relevant formats of this clone) include: immunohistochemistry of acetone-fixed frozen sections¹ and formaldehyde- fixed paraffin embedded sections^4,5 immunofluorescence microscopy³, in vivo activation of T cell responses¹, and in vivo inhibition of T cell responses².
RRID: AB_2860751 (BioLegend Cat. No. 201415)
AB_2860751 (BioLegend Cat. No. 201416)

Antigen Details

Structure: Ig superfamily, approximately 20-25 kD
Distribution: Thymocytes, peripheral T cells, dendritic epidermal T cells, NK-T cells
Function: Antigen recognition, TCR signal transduction, T cell activation
Ligand/Receptor: Peptide antigen/MHC complex
Cell Type: NKT cells, T cells, Thymocytes
Biology Area: Immunology
Molecular Family: CD Molecules
Antigen References: 1. Tanaka T, et al. 1989 J. Immunol. 142:2791.
2. Elbe A, et al. 1993. J. Invest. Dermatol. 102:74.
Gene ID: 25710 View all products for this Gene ID 300678 View all products for this Gene ID 315609 View all products for this Gene ID 25300 View all products for this Gene ID
UniProt: View information about CD3 on UniProt.org

Documentation

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Related Protocols

Cell Surface Flow Cytometry Staining Protocol

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Related Pages & Pathways

Pathways

Related FAQs

Do you guarantee that your antibodies are totally pathogen free?: BioLegend does not test for pathogens in-house aside from the GoInVivo™ product line. However, upon request, this can be tested on a custom basis with an outside, independent laboratory.
Does BioLegend test each Ultra-LEAF™ antibody by functional assay?: No, BioLegend does not test Ultra-LEAF™ antibodies by functional assays unless otherwise indicated. Due to the possible complexities and variations of uses of biofunctional antibodies in different assays and because of the large product portfolio, BioLegend does not currently perform functional assays as a routine QC for the antibodies. However, we do provide references in which the antibodies were used for functional assays and we do perform QC to verify the specificity and quality of the antibody based on our strict specification criteria.
Does BioLegend test each Ultra-LEAF™ antibody for potential pathogens?: No, BioLegend does not test for pathogens in-house unless otherwise indicated. However, we can recommend an outside vendor to perform this testing as needed.
Have you tested this Ultra-LEAF™ antibody for in vivo or in vitro applications?: We don't test our antibodies for in vivo or in vitro applications unless otherwise indicated. Depending on the product, the TDS may describe literature supporting usage of a particular product for bioassay. It may be best to further consult the literature to find clone specific information.

Other Formats

View All CD3 Reagents Request Custom Conjugation

Description	Clone	Applications
Purified anti-rat CD3	1F4	FC,IHC-F
FITC anti-rat CD3	1F4	FC
PE anti-rat CD3	1F4	FC
Alexa Fluor® 488 anti-rat CD3	1F4	FC,IHC-F
Alexa Fluor® 647 anti-rat CD3	1F4	FC,IHC-F
APC anti-rat CD3	1F4	FC
Ultra-LEAF™ Purified anti-rat CD3	1F4	FC,IHC-F
PerCP/Cyanine5.5 anti-rat CD3	1F4	FC
PE/Cyanine5 anti-rat CD3	1F4	FC
PE/Cyanine7 anti-rat CD3	1F4	FC
Spark Violet™ 423 anti-rat CD3	1F4	FC

Certificate of Analysis

Go To Top Version: 1 Revision Date: 03.23.2020

For Research Use Only. Not for diagnostic or therapeutic use.

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

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Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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