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APC/Cyanine7 anti-rat CD4 Antibody

Pricing & Availability
Clone
W3/25 (See other available formats)
Regulatory Status
RUO
Other Names
T4, L3T4, W3/25
Isotype
Mouse IgG1, κ
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Product Citations
publications
W3slash25_APCCy7_022309.jpg
LOU rat splenocytes stained with W3/25 APC/Cyanine7
  • W3slash25_APCCy7_022309.jpg
    LOU rat splenocytes stained with W3/25 APC/Cyanine7
Compare all formats See APC/Cyanine7 spectral data
Cat # Size Price Quantity Check Availability Save
201517 25 µg 132€
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201518 100 µg 278€
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Description

CD4 is a 55 kD glycoprotein also known as T4. Rat CD4 is a member of the immunoglobulin superfamily and is expressed on majority of thymocytes, macrophages, and a peripheral T cell subset (T helper cells). CD4 is a T cell co-receptor that interacts with the MHC class II molecule and is involved in T cell activation. 

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Rat
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Rat thymocyte membrane glycoproteins
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with APC/Cyanine7 under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.5 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Red Laser (633 nm)
Application Notes

The W3/25 antibody has been shown to inhibit IL-2 production by T helper cells and to prevent autoimmune T cell transfer in an MBP induced EAE model in vivo. Additional reported applications (for the relevant formats) include: immunohistochemistry of acetone-fixed frozen sections1,2, inhibition of IL-2 production3, inhibition of MBP-induced T cell activation in EAE transfer model3.

Application References

(PubMed link indicates BioLegend citation)
  1. Whiteland JL, et al. 1995. J. Histochem. Cytochem. 43:313. (IHC)
  2. Shioji K, et al. 2001. Circulation Res. 89:540. (IHC)
  3. Mannie MD, et al. 1993. J. Immunol. 151:7293.
  4. Kurtz CC, et al. 2007. Dev. Comp. Immunol. 31:415. PubMed
Product Citations
  1. Xie L, et al. 2014. J Immunol. 192:6009. PubMed
  2. Fehrenbach DJ, et al. 2020. Exp Physiol. 105:864. PubMed
  3. Abais-Battad JM, et al. 2021. Acta Physiol (Oxf). 232:e13662. PubMed
  4. Pai AV, et al. 2018. Physiology (Bethesda). 33:254. PubMed
  5. Yanai H, et al. 2022. Elife. 11:. PubMed
  6. Alsheikh AJ, et al. 2020. Am J Physiol Renal Physiol. F982:318. PubMed
  7. Li B, et al. 2020. Cell Mol Immunol. 1.386111111. PubMed
  8. Dasinger JH, et al. 2021. Pregnancy Hypertens. 24:126. PubMed
  9. Almolda B, et al. 2011. PLoS One. 6:e27473. PubMed
  10. Alsheikh AJ, et al. 2019. Am J Physiol Regul Integr Comp Physiol. 317:R182. PubMed
  11. Ruppert KA, et al. 2018. Sci Rep. 8:480. PubMed
RRID
AB_1186084 (BioLegend Cat. No. 201517)
AB_1186084 (BioLegend Cat. No. 201518)

Antigen Details

Structure
Ig superfamily, 55 kD
Distribution

Majority of thymocytes, macrophages, and a peripheral T cell subset (T helper cells)

Function
T cell co-receptor, T cell activation
Ligand/Receptor
MHC class II molecule
Cell Type
Macrophages, T cells, Thymocytes
Biology Area
Immunology
Molecular Family
CD Molecules
Antigen References

1. Brideau RJ, et al. 1980. Eur. J. Immunol. 10:609.
2. Clark SJ, et al. 187. P. Natl. Acad. Sci. USA 84:1649.

Gene ID
24938 View all products for this Gene ID 24939 View all products for this Gene ID
UniProt
View information about CD4 on UniProt.org
Go To Top Version: 1    Revision Date: 11.30.2012

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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