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Brilliant Violet 421™ anti-T-bet Antibody

Pricing & Availability
Clone
4B10 (See other available formats)
Regulatory Status
RUO
Other Names
T-box expressed in T cells, T box 21, TBLYM
Isotype
Mouse IgG1, κ
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Product Citations
publications
4B10_BV421_T-Bet_Antibody_ICFC_1_021715
Human peripheral blood lymphocytes were surface stained with CD3 APC and then treated with True-Nuclear™ Transcription Factor Buffer Set (Cat# 424401). Cells were then stained with T-bet (clone 4B10) Brilliant Violet 421™ (top) or mouse IgG1, κ Brilliant Violet 421™ isotype control (bottom).
  • 4B10_BV421_T-Bet_Antibody_ICFC_1_021715
    Human peripheral blood lymphocytes were surface stained with CD3 APC and then treated with True-Nuclear™ Transcription Factor Buffer Set (Cat# 424401). Cells were then stained with T-bet (clone 4B10) Brilliant Violet 421™ (top) or mouse IgG1, κ Brilliant Violet 421™ isotype control (bottom).
  • 4B10_BV421_T-Bet_Antibody_ICFC_2_021715
Compare all formats See Brilliant Violet 421™ spectral data
Cat # Size Price Quantity Check Availability Save
644815 125 µL 172€
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644832 50 µg 229€
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644816 500 µL 322€
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Description

T-bet, also known as T-box transcription factor T-bet, is considered to be a "master regulator" of Th1 lymphoid development controlling the production of the cytokine IFN-γ. T-bet is widely expressed in hematopoietic cells including stem cells, NK cells, B cells, and T cells. T-bet is critical for the control of microbial pathogens, and knockout animals show multiple physiologic and inflammatory features characteristic of asthma. T-bet expression is optimally observed after IL-12 stimulation and can be suppressed by addition of the Th2 cytokine IL-4 or neutralization of IL-12.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human, Mouse
Antibody Type
Monoclonal
Host Species
Mouse
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).
Preparation
The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 421™ under optimal conditions.
Concentration
µg sizes: 0.2 mg/mL
µL sizes: lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

ICFC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by intracellular immunofluorescent staining using our True-Nuclear™ Transcription Factor Staining Protocol. For flow cytometric staining using the µl size, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood. For flow cytometric staining using the µg size, the suggested use of this reagent is ≤ 0.4 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Brilliant Violet 421™ excites at 405 nm and emits at 421 nm. The standard bandpass filter 450/50 nm is recommended for detection. Brilliant Violet 421™ is a trademark of Sirigen Group Ltd.


Learn more about Brilliant Violet™.

This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents.
Excitation Laser
Violet Laser (405 nm)
Application Notes

Additional reported applications (for the relevant formats) include: immunoprecipitation2 and immunofluorescence microscopy3.

NOTE: For flow cytometric staining with this clone, True-Nuclear™ Transcription Factor Buffer Set (Cat. No. 424401) offers improved staining and is highly recommended over the Foxp3 Fix/Perm Buffer Set (Cat. No. 421403) and the Nuclear Factor Fixation and Permeabilization Buffer Set (Cat. No. 422601).

Application References

(PubMed link indicates BioLegend citation)
  1. Szabo SJ, et al. 2000. Cell 100:655. (ICFC, WB)
  2. Hwang ES, et al. 2005. J. Exp. Med. 202:1289. (ICFC, WB, IP)
  3. Neurath MF, et al. 2002. J. Exp. Med. 195:1129. (IF)
  4. Hsieh CY, et al. 2012. J Pharmacol Exp. 343:125. PubMed.
Product Citations
  1. Barber-Axthelm IM, et al. 2023. iScience. 26:106269. PubMed
  2. Pieren DK, et al. 2023. Nat Commun. 14:1887. PubMed
  3. Tan TCJ, et al. 2022. Nat Commun. 13:7796. PubMed
  4. Glaubitz J, et al. 2023. Gut. :. PubMed
  5. Dean JW 2023. Cell Reports. 42(1):111963. PubMed
  6. Hailemichael Y, et al. 2022. Cancer Cell. 40:509. PubMed
  7. Harsha Krovi S, et al. 2020. Nat Commun. 4.790277778. PubMed
  8. Gerber AN, et al. 2021. Cell Rep. 37:109816. PubMed
  9. Otano I, et al. 2021. Nat Commun. 12:7296. PubMed
  10. Wragg KM, et al. 2020. Cell Reports. 31(11):107773. PubMed
  11. Han SJ et al. 2017. Immunity. 47(6):1154-1168 . PubMed
  12. Saleh MM, et al. 2019. Cell Host Microbe. 25:756. PubMed
  13. McLane LM, et al. 2021. Cell Reports. 35(6):109120. PubMed
  14. Clement M, et al. 2016. PLoS Pathog. 12:e1006050. PubMed
  15. Glaubitz J, et al. 2022. Nat Commun. 13:4502. PubMed
  16. Aalderen M, et al. 2015. J Virol. 89:5110. PubMed
  17. Yasuoka T, et al. 2016. PLoS One. 11: 0157395. PubMed
  18. Wang HQ, et al. 2021. Cancer Research. 81(11):3079-3091. PubMed
  19. Prodinger J, et al. 2016. J Leukoc Biol. 99: 583-594. PubMed
  20. Marquardt N, et al. 2019. Nat Commun. 10:3841. PubMed
  21. Singh PP, et al. 2021. Ocul Surf. 21:271. PubMed
  22. Rodda LB, et al. 2020. Cell. 184(1):169-183.e17. PubMed
  23. Darragh LB, et al. 2022. Nat Commun. 13:7015. PubMed
  24. Linehan JL et al. 2018. Cell. 172(4):784-796 . PubMed
  25. Alhaj Hussen K, et al. 2017. Immunity. 47:680. PubMed
  26. Lorentsen KJ, et al. 2018. Nat Commun. 9:1679. PubMed
  27. Ganguly S, et al. 2021. Cell Mol Gastroenterol Hepatol. 12:891. PubMed
  28. Schaik B, et al. 2015. J Virol. 89:568. PubMed
  29. Sendler M, et al. 2020. Gastroenterology. 158:253. PubMed
  30. Hombrink P, et al. 2016. Nat Immunol. 17:1467-1478. PubMed
  31. Toshiro Hirai et al. 2019. Immunity. 50(5):1249-1261 . PubMed
  32. Phalke SP, et al. 2019. PLoS One. 14:e0218827. PubMed
  33. Roybal KT et al. 2016. Cell. 167(2):419-432 . PubMed
  34. Cheng J, et al. 2022. iScience. 25:103588. PubMed
  35. Clancy–Thompson E, et al. 2019. EMBO J. 38:e101260. PubMed
  36. Collins N, et al. 2020. Cell. 178(5):1088-1101.e15.. PubMed
  37. Berrien–Elliott MM, et al. 2020. Immunity. 51(3):479-490. PubMed
  38. Chao JL, et al. 2021. Cell Rep Med. 2:100399. PubMed
  39. Di Pilato M, et al. 2021. Cell. 184(17):4512-4530.e22. PubMed
  40. Aalderen M, et al. 2013. J Virol. 87:10263. PubMed
  41. Hirota K et al. 2018. Immunity. 48(6):1220-1232 . PubMed
  42. Goc J, et al. 2021. Cell. 184:5015. PubMed
  43. Song W, et al. 2022. Immunity. 55:290. PubMed
  44. Schreurs RRCE, et al. 2019. Immunity. 50:462. PubMed
  45. Shao TY et al. 2019. Cell host & microbe. 25(3):404-417 . PubMed
RRID
AB_2686976 (BioLegend Cat. No. 644815)
AB_2686976 (BioLegend Cat. No. 644832)
AB_2686976 (BioLegend Cat. No. 644816)

Antigen Details

Structure
T-box transcription factor, approximately 58 kD.
Distribution

Nuclear; expressed in T cells, hematopoietic stem cells, NK cells, B cells, lung, spleen.

Function
Th1-specific T-box transcription factor controlling expression of the hallmark Th1 cytokine, interferon gamma (IFN-γ). T-bet expression is critical for the control of microbial pathogens.
Cell Type
B cells, Hematopoietic stem and progenitors, NK cells, T cells, Tregs
Biology Area
Cell Biology, Immunology, Transcription Factors
Molecular Family
Nuclear Markers
Antigen References

1. Szabo SJ, et al. 2000. Cell 100:655.
2. Szabo SJ, et al. 2002. Science 295:338.
3. Finotto S, et al. 2002. Science 295:336.
4. Mullen AC, et al. 2001. Science 292:1907.

Gene ID
30009 View all products for this Gene ID
UniProt
View information about T-bet on UniProt.org

Related FAQs

What is the F/P ratio range of our BV421™ format antibody reagents?

It is lot-specific. On average it ranges between 2-4.

Go To Top Version: 4    Revision Date: 02.17.2015

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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