GoInVivo™ Purified anti-mouse CD152 Antibody

Product Details

Verified Reactivity

Mouse

Antibody Type

Monoclonal

Host Species

Syrian Hamster

Immunogen

Mouse CTLA-4-human IgG1 fusion protein

Formulation

0.2 µm filtered in phosphate-buffered solution, pH 7.2, containing no preservative.

Endotoxin Level

Less than 1.0 EU/mg of the protein (< 0.1 pg/µg of the protein) as determined by the LAL test.

Preparation

The GoInVivo™ antibody was purified by affinity chromatography.

Concentration

The antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL.

Storage & Handling

The antibody solution should be stored undiluted between 2°C and 8°C. This GoInVivo™ solution contains no preservative; handle under aseptic conditions.

Application

ELISA - Quality tested
Block, Costim, FC - Reported in the literature, not verified in house

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume or 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.

For in vivo and in vitro applications, we recommend to perform a pilot experiment to determine the optimal concentration to use for each particular experiment.

Application Notes

Additional reported applications (for the relevant formats) include: in vivo and in vitro blocking^1-4 of CTLA-4 action by soluble antibody and promotion of in vitro CTLA-4 signalling by immobilized antibody¹. To reduce non-specific binding to cells bearing Fc-receptors, pre-incubation of cells with anti-mouse CD16/CD32, clone 93 (Cat. No. 101301/101302), is recommended prior to immunofluorescent staining. For most successful immunofluorescent staining results, it may be important to maximize signal over background by using a relatively bright fluorochrome-antibody conjugate or by using a high sensitivity, three-layer staining technique (e.g., including a biotinylated anti-Syrian hamster IgG (Cat. No. 405601) second step, followed by SAv-PE (Cat. No. 405204)).

Additional Product Notes

GoInVivo™ products are guaranteed to be pathogen-free based on the IDEXX BioResearch IMPACT test via PCR. For a full listing of pathogens tested, visit the GoInVivo™ Webpage.

Application References

(PubMed link indicates BioLegend citation)

1. Krummel MF, et al. 1995. J. Exp. Med. 182:459. (Block, Costim, FC)
2. Krummel MF, et al. 1996. Int. Immunol. 8:519. (Block)
3. Leach DR, et al. 1996. Science 271:1734. (Block)
4. Wagner Jr, DH, et al. 1996. J. Exp. Med. 184:1631. (Block)

Product Citations

1. Wang Y, et al. 2016. Exp Ther Med. 81:12. PubMed
2. Zhu Y, et al. 2023. Sci Immunol. eade4656:8. PubMed

RRID

AB_2616629 (BioLegend Cat. No. 106210)
AB_2616629 (BioLegend Cat. No. 106206)
AB_2616629 (BioLegend Cat. No. 106209)
AB_2616629 (BioLegend Cat. No. 106207)
AB_2616629 (BioLegend Cat. No. 106208)
AB_2616629 (BioLegend Cat. No. 106205)

Disclaimer

This product may be used for research purposes only. It is not licensed for resale and may only be used by the buyer. This product may not be used and is not licensed for clinical assays where the results of such assays are provided as a diagnostic service. If a diagnostic or therapeutic use is anticipated then a license must be requested from the University of California. The availability of such diagnostic and therapeutic use license(s) cannot be guaranteed from the University of California.

Antigen Details

Structure

Ig superfamily, 33 kD

Distribution

Activated T cells

Function

Negative regulator of T cell activation

Ligand/Receptor

CD80 (B7-1), CD86 (B7-2)

Cell Type

T cells, Tregs

Biology Area

Immunology

Molecular Family

CD Molecules, Immune Checkpoint Receptors

Antigen References

1. Barclay A, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Allison JP, et al. 1995. Science 270:932.
3. Waterhouse P, et al. 1995. Science 270:985.
4. Linsley PS, et al. 1991. J. Exp. Med. 174:561.

Gene ID

12477 View all products for this Gene ID

UniProt

View information about CD152 on UniProt.org

Documentation

• Technical Data Sheet (pdf)
• Certificate of Analysis
• Safety Data Sheet

Reviews

Related Protocols

• Cell Surface Flow Cytometry Staining Protocol

Related Pages & Pathways

Related FAQs

Other Formats

Certificate of Analysis