Loading...

PE anti-mouse CD150 (SLAM) Antibody

Pricing & Availability
Clone
TC15-12F12.2 (See other available formats)
Regulatory Status
RUO
Other Names
Signaling Lymphocyte Activation Molecule (SLAM), IPO-3
Isotype
Rat IgG2a, λ
Ave. Rating
Submit a Review
Product Citations
publications
TC15-12F12.2
C57BL/6 mouse splenocytes were stained with CD150 (clone TC15-12F12.2) PE (solid line) or rat IgG2a PE isotype control (broken line).
  • TC15-12F12.2
    C57BL/6 mouse splenocytes were stained with CD150 (clone TC15-12F12.2) PE (solid line) or rat IgG2a PE isotype control (broken line).
  • TC15-12F12_PE_2_102110
    C57BL/6 mouse bone marrow cells were stained with CD150 (clone TC15-12F12.2) PE (filled histogram) or rat IgG2a PE isotype control (open histogram) (gated on lymphoid cell population).
Compare all formats See PE spectral data
Cat # Size Price Quantity Check Availability Save
115903 25 µg 84€
Check Availability


Need larger quantities of this item?
Request Bulk Quote
115904 100 µg 215€
Check Availability


Need larger quantities of this item?
Request Bulk Quote
Description

CD150 is a 75-95 kD member of the immunoglobulin superfamily, also known as SLAM (signaling lymphocyte activation molecule) or IPO-3. CD150, a single chain type I transmembrane molecule, is expressed on thymocytes, T cell subsets, B cells, dendritic cells, and endothelial cells. The expression is upregulated upon activation. CD150 expression has been shown to be maintained on Th1 but not Th2 clones. T regulatory cells express a relatively high level of CD150. Antibodies against CD150 have been shown to augment IFN-γ production by Th1 cells, especially when co-stimulated through the TCR. CD150 associates with the src homology 2-domain-containing protein tyrosine phosphatase SHP-2, and this association is thought to be involved in signal transduction. In combination with CD48, CD150 is a useful marker for hematopoietic stem cell studies.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Mouse SLAM-human IgG1 fusion protein
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The CD150 antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

The TC15-12F12.2 antibody has been reported to enhance the production of IFN-? by Th1 cells stimulated through TCR. Additional reported applications (for the relevant formats) include: immunoprecipitaion17, enhancing IFN-? production by Th1 cells when stimulated with CD31, and inhibiting CD3 induced T cell proliferation6. The Ultra-LEAF™ purified antibody (Endotoxin <0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 115949 & 115950).

Application References

(PubMed link indicates BioLegend citation)
  1. Castro AG, et al. 1999. J. Immunol. 163:5860. (FC, Costim, IP)
  2. Forsberg EC, et al. 2005. PLoS Genet. 1:e28. (FC)
  3. Terrazas LI, et al. 2005. Int. J. Parasitol. 35:1349. (FC)
  4. Cannons JL, et al. 2006. J. Exp. Med. 203:1551. (FC)
  5. Umemoto T, et al. 2006. J. Immunol. 177:7733. (FC)
  6. Jordan MA, et al. 2007. J. Immunol. 178:1618. (FC, Block) PubMed
  7. Jung Y, et al. 2007. Blood 110:82. PubMed
  8. Pimanda JE, et al. 2007. Proc. Natl. Acad. Sci. USA 104:840.
  9. Sugiyama T, et al. 2007. Proc. Natl. Acad. Sci. USA 104:175.
  10. Kim I, et al. 2006. Blood 108:737. PubMed
  11. Ema H, et al. 2006. Nat Protoc. 1:2979. PubMed
  12. Fraser ST, et al. 2007. Blood 109:4616. PubMed
  13. Jung Y, et al. 2008. Stem Cells. 26:2042. Pubmed
  14. Song J, et al. 2010. Blood 115:2592. PubMed
  15. Cridland SO, et al. 2009. Blood Cell. Mol. Dis. 43:149. (FC) PubMed
  16. Morita Y, et al. 2010. J. Exp Med. 207:1173. PubMed
  17. Talaei N, et al. 2015. J. Immunol. 195(10):4623. PubMed
Product Citations
  1. Soukup AA, et al. 2019. J Clin Invest. 129:1180. PubMed
  2. Hu W, et al. 2015. Blood. 125:2206. PubMed
  3. Pietras E, et al. 2016. Nat Cell Biol. 10.1038/ncb3346. PubMed
  4. Chen Q, et al. 2019. Cell Stem Cell. 25(6):768-783.e6. PubMed
  5. Grigsby SM, et al. 2021. Cancers (Basel). 13:. PubMed
  6. Ho TT, et al. 2021. J Exp Med. 218:. PubMed
  7. Zhang D, et al. 2022. Cell Stem Cell. 29:232. PubMed
  8. Mirchandani AS, et al. 2022. Nat Immunol. 23:927. PubMed
  9. Tátrai P, et al. 2022. EMBO J. 41:e108739. PubMed
  10. Xu Y, et al. 2023. EMBO Rep. 24:e55503. PubMed
  11. Huang D, et al. 2023. Exp Hematol Oncol. 12:11. PubMed
  12. Yuan O, et al. 2022. Sci Adv. 8:eabm9987. PubMed
  13. Meacham CE, et al. 2022. Nat Cell Biol. 24:697. PubMed
  14. Jabbar S, et al. 2022. Exp Hematol Oncol. 11:83. PubMed
  15. Shiroshita K, et al. 2022. Cell Rep Methods. 2:100354. PubMed
  16. Kang YA, et al. 2023. J Exp Med. 220:. PubMed
  17. Meng Y, et al. 2023. Cell Mol Biol Lett. 28:45. PubMed
  18. Hirata Y et al. 2018. Cell stem cell. 22(3):445-453 . PubMed
  19. Nita A, et al. 2021. Cell Reports. 34(5):108688. PubMed
  20. Maltby S, et al. 2014. J Immunol. 193:4072. PubMed
  21. Bowers E, et al. 2018. Nat Med. 24:95. PubMed
  22. Thomas GE, et al. 2022. Nat Cell Biol. 24:872. PubMed
  23. Yang X, et al. 2020. Blood Sci. 2:89. PubMed
  24. Wei Q, et al. 2021. Nat Commun. 12:2522. PubMed
  25. Gao B, et al. 2018. Aging Cell. 3. PubMed
  26. Zhao M, et al. 2019. Cell Rep. 26:652. PubMed
  27. Fukushima T, et al. 2019. Cell Rep. 29:4144. PubMed
  28. Kobayashi H, et al. 2020. STAR Protoc. 1:100078. PubMed
  29. Jung Y, et al. 2008. Stem Cells. 26:2042. PubMed
  30. Liu X, et al. 2021. eLife. 0.416666666666667. PubMed
  31. Yamamoto K, et al. 2021. Cell Reports. 36(8):109576. PubMed
  32. Fujino T, et al. 2021. Nat Commun. 12:1826. PubMed
  33. Li CC, et al. 2022. Nat Commun. 13:346. PubMed
  34. Vannini N, et al. 2019. Cell Stem Cell. 24:405. PubMed
  35. Nakamura‐Ishizu A et al. 2018. Cell reports. 25(7):1772-1785 . PubMed
  36. Kokkaliaris K, et al. 2016. Blood. 128: 1181 - 1192. PubMed
  37. Greenbaum A, et al. 2012. Blood. 120:295. PubMed
  38. Ganuza M, et al. 2017. Exp Hematol. 10.1016/j.exphem.2017.04.006. PubMed
  39. Stivala S, et al. 2019. J Clin Invest. 130:1596. PubMed
  40. Baccelli I, et al. 2020. Cancer Cell. 36(1):84-99. PubMed
  41. Hillel–Karniel C, et al. 2020. Cell Reports. 30(3):807-819.e4.. PubMed
  42. Kim SP, et al. 2021. Cell Reports. 36(9):109626. PubMed
  43. Jordan M, et al. 2007. J Immunol. 178:1618. PubMed
  44. Chen X et al. 2017. Cell stem cell. 21(6):747-760 . PubMed
  45. Mooney C, et al. 2017. International Journal of Molecular Sciences. 10.3390/ijms18051037. PubMed
  46. Leiva M, et al. 2016. Nat Commun. 7:10222. PubMed
  47. Grassinger J, et al. 2012. Cytokine. 58:218. PubMed
  48. Jung Y, et al. 2011. Exp Hematol. 39:151. PubMed
  49. Kinkel SA, et al. 2022. iScience. 25:104684. PubMed
  50. Jia W, et al. 2021. Nat Commun. 12:2118. PubMed
  51. Breitbach M, et al. 2018. Cell Stem Cell. 1.098611111. PubMed
  52. Tadokoro Y, et al. 2018. Cell Stem Cell. 1.411805556. PubMed
  53. Ise W, et al. 2018. Immunity. 48:702. PubMed
  54. Comazzetto S et al. 2018. Cell stem cell. 24(3):477-486 . PubMed
  55. Kim I, et al. 2006. Blood. 108:737. PubMed
  56. Zhang J, et al. 2015. Leukemia. 29 1847 . PubMed
  57. Bahal R, et al. 2016. Nat Commun. 7:13304. PubMed
  58. Li Y, et al. 2020. Cell Stem Cell. 27(5):732-747.e7. PubMed
  59. Dong S, et al. 2021. Nature. 591:117. PubMed
  60. Morita Y, et al. 2010. J Exp Med. 207:1173. PubMed
  61. Jung Y, et al. 2007. Blood . 110:82. PubMed
  62. Nagai M, et al. 2020. Cell. 178(5):1072-1087.e14.. PubMed
  63. Hsu JI et al. 2018. Cell stem cell. 23(5):700-713 . PubMed
  64. Reismann D, et al. 2017. Nat Commun.. 10.1038/s41467-017-01538-9. PubMed
  65. Witkowski MT, et al. 2020. Cancer Cell. 37:867. PubMed
  66. Montecino-Rodriguez E, et al. 2020. STAR Protoc. 1:100159. PubMed
  67. Chiba H, et al. 2013. Am J Physiol Cell Physiol. 305:693. PubMed
  68. Pinho S, et al. 2022. Nat Cell Biol. 24:290. PubMed
  69. Ye P, et al. 2022. Front Cardiovasc Med. 8:810477. PubMed
  70. Seneviratne AK, et al. 2019. Cell Stem Cell. 24:621. PubMed
  71. Nacson J, et al. 2020. Molecular Cell. 78(5):951-959. PubMed
  72. Du J, et al. 2012. Stem Cells. 30:1447. PubMed
  73. Nosho S, et al. 2020. Int J Mol Sci. 21:00. PubMed
  74. Kruta M, et al. 2021. Cell Stem Cell. :. PubMed
  75. Song J, et al. 2010. Blood. 115:2592. PubMed
  76. van Oostrum M, et al. 2019. Nat Commun. 4.398611111. PubMed
  77. Williams JW, et al. 2017. Circ Res. 121:662. PubMed
  78. Pierce H, et al. 2017. Cell Stem Cell. 1.283333333. PubMed
  79. Chen Z, et al. 2019. J Exp Med. 216:152. PubMed
  80. Pinho S et al. 2018. Developmental cell. 44(5):634-641 . PubMed
  81. Xia P et al. 2018. Immunity. 48(4):688-701 . PubMed
  82. Yi W, et al. 2021. Cell Reports. 34(13):108922. PubMed
  83. Yang S, et al. 2022. J Exp Med. 219:. PubMed
  84. Sacma M, et al. 2022. STAR Protoc. 3:101483. PubMed
  85. Philip E Boulais et al. 2018. Immunity. 49(4):627-639 . PubMed
  86. Balzano M et al. 2019. Cell reports. 26(12):3257-3271 . PubMed
  87. Kobayashi H, et al. 2020. Cell Reports. 28(1):145-158.e9.. PubMed
  88. Garaycoechea JI, et al. 2018. Nature. 553:171. PubMed
  89. Thongthip S, et al. 2016. Genes Dev. 30: 645 - 659. PubMed
  90. Wang X, et al. 2018. Stem Cell Reports. 11:274. PubMed
  91. Wei Q, et al. 2020. Dev Cell. 53:503. PubMed
  92. Chen R, et al. 2021. Cell Reports. 34(7):108751. PubMed
  93. Matsumura T, et al. 2022. Nat Commun. 13:7064. PubMed
  94. Zhang CR, et al. 2022. Blood Cancer Discov. 3:220. PubMed
  95. Celik H, et al. 2018. Cancer Cell. 34:741. PubMed
  96. Kaplan I, et al. 2011. J Immunol. 186:2826. PubMed
  97. Zhang J, et al. 2015. J Biol Chem. 290 19093 . PubMed
  98. Ley C, et al. 2015. Cancer Cell. 27: 631-43. PubMed
  99. Keerthivasan S, et al. 2021. Immunity. 54(7):1511-1526.e8. PubMed
  100. Hoffmann J, et al. 2021. Nat Commun. 12:3964. PubMed
  101. Fischer KC, et al. 2021. Cell Death Dis. 12:28. PubMed
  102. Omatsu Y, et al. 2022. Nat Commun. 13:2654. PubMed
  103. He M, et al. 2020. Cell Metabolism. 31(3):580-591. PubMed
  104. Xu C, et al. 2018. Nat Commun. 9:2449. PubMed
  105. Alvarez S, et al. 2015. Nat Commun. 6: 8548. PubMed
  106. Theocharides A, et al. 2012. J Exp Med. 209:1883. PubMed
  107. Soukup AA, et al. 2021. Sci Adv. 7:eabk3521. PubMed
  108. Gao X, et al. 2021. Nature. 589:591. PubMed
  109. Yoshida H, et al. 2019. Cell. 176:897. PubMed
  110. DeVilbiss AW, et al. 2021. eLife. 10:00. PubMed
  111. Xu W, et al. 2021. Immunity. 54(3):526-541.e7. PubMed
  112. Magee JA, et al. 2021. Stem Cell Reports. 16:20. PubMed
  113. Woods B, et al. 2019. Clin Cancer Res. 25:5901. PubMed
  114. Li K, et al. 2020. Nat Commun. 0.795138889. PubMed
  115. Hidalgo San Jose L, et al. 2020. Cell Rep. 30:69. PubMed
  116. Yamashita M, et al. 2019. Cell Stem Cell. 25:357. PubMed
  117. Säwen P et al. 2018. eLife. 7 pii: e41258. PubMed
  118. Luo H, et al. 2019. Cell Rep. 26:945. PubMed
  119. Riedel SS, et al. 2021. Molecular Cell. 81(11):2332-2348.e9. PubMed
  120. Matsuzaki Y, et al. 2015. Biomed Rep. 1: 91 - 97. PubMed
  121. Zhou J, et al. 2019. Cell Stem Cell. 24:285. PubMed
  122. Fraser S, et al. 2007. Blood. 109:343. PubMed
  123. Asada N, et al. 2017. Nat Cell Biol. 19:214-223. PubMed
  124. Kang YA, et al. 2020. J Exp Med. 217:00:00. PubMed
  125. Xie M, et al. 2020. Haematologica. . PubMed
  126. Yang L, et al. 2021. Front Immunol. 12:722273. PubMed
  127. Tan DQ, et al. 2019. Cell Rep. 26:2316. PubMed
  128. Holzapfel B, et al. 2015. Biomaterials. 61: 103-114. PubMed
  129. Man N, et al. 2021. JCI Insight. 6:. PubMed
  130. Gu Z, et al. 2021. Nat Genet. 53:672. PubMed
RRID
AB_313682 (BioLegend Cat. No. 115903)
AB_313682 (BioLegend Cat. No. 115904)

Antigen Details

Structure
Ig superfamily, 75-95 kD
Distribution

Thymocytes, T cell subset, B lymphocytes, dendritic cells, endothelial cells

Function
B cell and dendritic cell costimulation
Ligand/Receptor
CD150
Cell Type
B cells, Dendritic cells, Endothelial cells, T cells, Thymocytes, Tregs
Biology Area
Costimulatory Molecules, Immunology, Innate Immunity
Molecular Family
Adhesion Molecules, CD Molecules
Antigen References

1. Cocks BG, et al. 1995. Nature 376:260.
2. Punnonen J, et al. 1997. J. Exp. Med. 185:993.
3. Sidorenko SP, et al. 1993. J. Immunol. 151:4614.

Gene ID
27218 View all products for this Gene ID
UniProt
View information about CD150 on UniProt.org

Related FAQs

What type of PE do you use in your conjugates?
We use R-PE in our conjugates.
Go To Top Version: 1    Revision Date: 11.30.2012

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

ProductsHere
Login / Register
Remember me
Forgot your password? Reset password?
Create an Account