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PE/Cyanine7 anti-human CD43 Antibody

Pricing & Availability
Clone
CD43-10G7 (See other available formats)
Regulatory Status
RUO
Other Names
Ly-48, Leukosialin, Sialophorin, Leukocyte Sialoglycoprotein
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
CD43-10G7_PECy7_CD43_Antibody_FC_061714.jpg
Human peripheral blood lymphocytes were stained with CD43 (clone CD43-10G7) PE/Cyanine7 or mouse IgG1, κ PE/Cyanine7 isotype control.
  • CD43-10G7_PECy7_CD43_Antibody_FC_061714.jpg
    Human peripheral blood lymphocytes were stained with CD43 (clone CD43-10G7) PE/Cyanine7 or mouse IgG1, κ PE/Cyanine7 isotype control.
Compare all formats See PE/Cyanine7 spectral data
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343207 25 tests 90€
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343208 100 tests 240€
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Description

CD43, also known as Ly-48, leukosialin, sialophorin, leukocyte sialoglycoprotein, and W3/13, is a large single chain, type I transmembrane glycoprotein with abundant O-glycosylation and sialylation sites. It has been reported that CD43 binds to CD54 and Siglec-1. CD43 plays dual roles in cell adhesion and anti-adhesion as well as costimulation of T cell activation and survival, and induction of apoptosis of T cells and hematopoietic progenitors.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Reported Reactivity
African Green, Baboon, Cynomolgus
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
MV4-11 cell line
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography and conjugated with PE/Cyanine7 under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application References

(PubMed link indicates BioLegend citation)
  1. Rosenkranz AR, et al. 1993. Immunology. 80:431
  2. Skubitz KM, et al. 1998. J. Leukoc. Biol. 64:800
Product Citations
  1. Iqneibi S, et al. 2023. Front Immunol. 1239148:14. PubMed
  2. Coley WD et al. 2018. F1000Research. 2018 Mar 14 [revised 2018 Jan 1] 0.5125. PubMed
  3. Matsuo S, et al. 2021. PLoS One. e0247595:16. PubMed
RRID
AB_2563698 (BioLegend Cat. No. 343207)
AB_2563698 (BioLegend Cat. No. 343208)

Antigen Details

Structure
Single chain, type I transmembrane glycoprotein with abundant O-glycosylation and sialylation sites, 95-130 kD.
Distribution

Most hematopoietic cells, including peripheral T cells, NK cells, granulocytes, monocytes, thymocytes, platelets, plasma cells, activated B cells, and hemotopoietic progenitor cells.

Function
Plays dual roles in cell-cell adhesion and anti-adhesion, costimulation of cell activation and survival, inducing apoptosis of T cells and hematopoietic progenitors.
Ligand/Receptor
CD54, Siglec-1
Biology Area
Immunology
Molecular Family
Adhesion Molecules, CD Molecules
Antigen References

1. Neil Barclay A, et al. 1997. The Leucocyte Antigen Facts Book:Second Edition. Page 238
2. Killeen A, et al. 1987. EMBO J. 6:4029.
3. Thurman EC, et al. 1998. Intl. Immunol. 10:691.
4. Onami TM, et al. 2002. J. Immunol. 168:6022.
5. Tong J, et al. 2004. J. Exp. Med. 199:1277.
6. Jones AT, et al. 1994. J. Immunol. 153:3426.

Gene ID
6693 View all products for this Gene ID
UniProt
View information about CD43 on UniProt.org
Go To Top Version: 1    Revision Date: 06.18.2014

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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