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Purified anti-NUP153 Antibody (Previously Covance catalog# MMS-102P)

Pricing & Availability
Clone
QE5 (See other available formats)
Regulatory Status
RUO
Other Names
HNUP153, N153, Nuclear Pore Complex Protein NUP153, Nucleoporin Nup153, 153 kD nucleoporin, nuclear pore complex protein hnup153
Previously
Covance Catalog# MMS-102P
Isotype
Mouse IgG1, κ
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Product Citations
publications
QE5_Purified_NUP153_Antibody_1_IHC_022415
Methanol fixed HeLa stained with the antibody QE5. This antibody brilliantly highlights the nuclear membrane (green). The golgi is stained with the antibody to Giantin.
  • QE5_Purified_NUP153_Antibody_1_IHC_022415
    Methanol fixed HeLa stained with the antibody QE5. This antibody brilliantly highlights the nuclear membrane (green). The golgi is stained with the antibody to Giantin.
  • QE5_Purified_NUP153_Antibody_2_ICC_022415
    Immunofluorescence of HeLa cells with (A) mouse IgG1, κ isotype control (Negative, Cat. No. 401402) or (B) NUP153 Mouse primary antibody (Clone QE5). 2 μg/ml (1:250 dilution) Alexa Fluor® 594 (Red) Goat anti-Mouse IgG (Cat. No. 405326) was used as secondary antibody. Nuclei were counterstained with DAPI (Blue, Cat. No. 422801). The image was captured with a 60X objective objective using KEYENCE BZ-X700 fluorescence microscope. Exposure time is 300 ms. Concentrations 4 µg/ml.
  • QE5_Purified_NUP153_Antibody_3_WB_022415
    Total lysates (15 µg protein) from Daudi, HEL (Human), COS-7 (Monkey), Raw264.7 (Mouse), UMR106 (Rat) and CHO (Hamster) were resolved by electrophoresis (4-20% Tris-glycine gel), transferred to nitrocellulose, and probed with 1:1000 (1 µg/ml) purified NUP153 antibody, clone QE5. Proteins were visualized using chemiluminescence detection by incubation with HRP Goat anti-Mouse secondary antibody (Cat. No. 405306, 1:3000 dilution). Direct-Blot™ HRP anti-&betal-actin was used as a loading control (Cat. No. 643807, 1:8000 dilution).
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906201 100 µL 203€
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Description

Nucleoporin 153 (Nup153) is a component of the nuclear pore complex, and is required for the anchoring of the nuclear pore complex to the nuclear pore membrane. Nup153 has a critical role in the nuclear export of proteins and RNA, and is involved in the retention of unspliced mRNAs in the nucleus.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human, Mouse, Rat, Non-Human Primate, Hamster
Reported Reactivity
Other species
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
The QE5 monoclonal antibody was generated against rat liver nuclear envelope proteins.
Formulation
Phosphate-buffered solution + 0.03% thimerosal.
Preparation
The antibody was purified by affinity chromatography.
Concentration
1 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C. Please note the storage condition for this antibody has been changed from -20°C to between 2°C and 8°C. You can also check your vial or your CoA to find the most accurate storage condition for this antibody.
Application

ICC - Quality tested
WB, IP - Verified
IEM - Reported in the literature, not verified in house
Recommended Usage

Each lot of this antibody is quality control tested by immunocytochemistry. For immunocytochemistry, a concentration of 4.0 μg/ml (1:250 dilution) is recommended. For Western blotting, the suggested use of this reagent is 1.0 - 2.0 µg per ml (1:500-1:1000 dilution). For immunoprecipitation, the suggested use of this reagent is 1:50 dilution. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

This antibody is effective in immunoblotting, immunocytochemistry (ICC) and immunoprecipitation (IP).

*Predicted MW = 250 kD

This antibody recognizes NUP153 as well as two related nuclear pore complex proteins: NUP214 and p62. By immunofluorescence, QE5 labels the nuclear envelope of eukaryotic cells giving a punctate staining pattern.
 

Predicted MW is ~ 250 kD, observed MW is ~154 kD. Lower bands (~ 60 kD) maybe the tripartite structure of NUP153, which consists of a zinc finger domain flanked by large (60-70 kD) NH2- and COOH-terminal domains9.

Application References

(PubMed link indicates BioLegend citation)
  1. Pare GC, et al. 2005. Exp Cell Res. 303:388.
  2. N Pante, et al. 1994. J Cell Biol 126:603.
  3. Smythe C, et al. 2000. EMBO J. 19:3918. (WB)
  4. Goujon C, et al. 2008. J Virol. 82:12335. (IF) PubMed
  5. Holzenspies JJ, et al. 2009. BMC Dev Biol.9:8. (IF) PubMed
  6. Green JE, et al. 2014. Dev Biol. 392:419. (IF) PubMed
  7. Chen CY, et al. 2012. Cell. 149:565. (IF, WB)
  8. Iborra FJ, et al.2000. J Cell Sci. 113:291. (IEM)
  9. Bastos R, et al. 1997. J Cell Biol. 137:989. (IF, IP)
Product Citations
  1. Hölzenspies J, et al. 2009. BMC Dev Biol. 9:8. PubMed
  2. Goujon C, et al. 2008. J Virol. 82:12335-12345. PubMed
  3. Akam J 2014. Dev Biol. 392:419-430. PubMed
  4. Liu X et al. 2017. Cell. 170(5):1028-1043 . PubMed
  5. Shindo Y, et al. 2016. Nat Commun. 7:10485. PubMed
RRID
AB_2565060 (BioLegend Cat. No. 906201)

Antigen Details

Biology Area
Cell Biology, Cell Motility/Cytoskeleton/Structure, Neuroscience, Neuroscience Cell Markers
Molecular Family
Nuclear Markers
Gene ID
9972 View all products for this Gene ID
UniProt
View information about NUP153 on UniProt.org
Go To Top Version: 5    Revision Date: 09.22.2023

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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