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PerCP/Cyanine5.5 anti-mouse CD3 Antibody

Pricing & Availability
Clone
17A2 (See other available formats)
Regulatory Status
RUO
Other Names
T cell antigen receptor complex, T3
Isotype
Rat IgG2b, κ
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Product Citations
publications
17A2_PerCPCyanine55_CD3_Antibody_020819_updated.png
C57BL/6 splenocytes were stained with CD45R/B220 APC and CD3 (clone 17A2) PerCP/Cyanine5.5 (left) or Rat IgG2b, κ PerCP/Cyanine5.5 isotype control (right).
  • 17A2_PerCPCyanine55_CD3_Antibody_020819_updated.png
    C57BL/6 splenocytes were stained with CD45R/B220 APC and CD3 (clone 17A2) PerCP/Cyanine5.5 (left) or Rat IgG2b, κ PerCP/Cyanine5.5 isotype control (right).
Compare all formats See PerCP/Cyanine5.5 spectral data
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100217 25 µg 95€
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100218 100 µg 259€
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Description

CD3, also known as T3, is a member of the Ig superfamily and primarily expressed on T cells, NK-T cells, and at different levels on thymocytes during T cell differentiation. CD3 is composed of CD3ε, δ, γ and ζ chains. It forms a TCR complex by associating with TCR α/β or γ/δ chains. CD3 plays a critical role in TCR signal transduction, T cell activation, and antigen recognition by binding the peptide/MHC antigen complex

Product Details
Technical data sheet

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
γδTCR-positive T-T hybridoma D1
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with PerCP/Cyanine5.5 under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is =1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

* PerCP/Cyanine5.5 has a maximum absorption of 482 nm and a maximum emission of 690 nm.

Application Notes

Additional reported application (for relevant formats) include: spatial biology (IBEX)1,2.

Application References

(PubMed link indicates BioLegend citation)
  1. Radtke AJ, et al. 2020. Proc Natl Acad Sci U S A. 117:33455-65. (SB) PubMed
  2. Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed
Product Citations
  1. Harsha Krovi S, et al. 2020. Nat Commun. 4.790277778. PubMed
  2. Hirai T, et al. 2020. Immunity. 54(1):84-98.e5. PubMed
  3. Chen Y, et al. 2020. Virol Sin. . PubMed
  4. Abraham A, et al. 2019. J Clin Invest. 130:2685. PubMed
  5. Li SX, et al. 2019. PLoS Pathog. 15:e1007611. PubMed
  6. Kawabe T, et al. 2020. Nat Commun. 2.795833333. PubMed
  7. Marchelletta RR, et al. 2021. J Clin Invest. 131:. PubMed
  8. Cautivo KM, et al. 2022. Immunity. 55:254. PubMed
  9. Wang H, et al. 2022. Immunol Cell Biol. 100:547. PubMed
  10. Zhao Z, et al. 2022. Adv Sci (Weinh). 9:e2201293. PubMed
  11. Rodrigues PB, et al. 2022. Cells. 11: . PubMed
  12. Chen X, et al. 2022. J Extracell Vesicles. 11:e12279. PubMed
  13. Choi H, et al. 2023. J Nanobiotechnology. 21:5. PubMed
  14. Domingos-Pereira S, et al. 2022. Int J Mol Sci. 24: . PubMed
  15. Arimoto KI, et al. 2023. Nat Commun. 14:251. PubMed
  16. Ivasko SM, et al. 2023. Front Immunol. 13:1023206. PubMed
  17. Cui B, et al. 2023. Int Immunopharmacol. 114:109541. PubMed
  18. Tunali G, et al. 2023. J Clin Invest. :. PubMed
  19. Dragan M, et al. 2022. J Invest Dermatol. 142:583. PubMed
  20. Alghamri MS, et al. 2022. ACS Nano. 16:8729. PubMed
  21. Lin J, et al. 2022. Adv Sci (Weinh). 9:e2202633. PubMed
  22. Schroeter CB, et al. 2022. J Neuroinflammation. 19:270. PubMed
  23. Li Y, et al. 2023. Adv Mater. 35:e2208923. PubMed
  24. Gao L, et al. 2023. J Nanobiotechnology. 21:56. PubMed
  25. Aso K, et al. 2023. Nat Commun. 14:984. PubMed
  26. Wang Y, et al. 2023. EBioMedicine. 90:104480. PubMed
  27. Tan J, et al. 2023. Acta Pharm Sin B. 13:632. PubMed
  28. Masle-Farquhar E, et al. 2023. Front Immunol. 14:1095257. PubMed
  29. Wiedmann L, et al. 2023. Nat Commun. 14:2353. PubMed
  30. Wang Y, et al. 2023. iScience. 26:106630. PubMed
  31. Wang K, et al. 2023. Nat Commun. 14:2950. PubMed
  32. Tsugawa H, et al. 2023. PLoS Pathog. 19:e1011139. PubMed
  33. Wang X, et al. 2023. Nat Commun. 14:3440. PubMed
  34. Zhou M, et al. 2023. Nat Commun. 14:3593. PubMed
  35. Song S, et al. 2023. Bioact Mater. 28:358. PubMed
  36. Guo X, et al. 2023. Mol Immunol. 160:32. PubMed
  37. McNamara HA, et al. 2020. Cell Host Microbe. 572:28. PubMed
  38. Kaczanowska S, et al. 2021. Cell. 184(8):2033-2052.e21. PubMed
  39. Viny AD, et al. 2019. Cell Stem Cell. 25:682. PubMed
  40. Ye Y, et al. 2022. Nat Commun. 13:6458. PubMed
  41. Li X, et al. 2022. Nat Commun. 13:2794. PubMed
  42. Riley JS, et al. 2020. Blood Adv. 4:1102. PubMed
  43. Liang S, et al. 2022. Acta Pharm Sin B. 12:2494. PubMed
  44. Pessoa Rodrigues C, et al. 2021. Sci Adv. 7:. PubMed
  45. Zhang S, et al. 2021. Cell. 184(8):2151-2166.e16. PubMed
  46. Eastman A, et al. 2016. J Immunol . 194: 5999-6010. PubMed
  47. Molofsky A, et al. 2013. J Exp Med. 210:535. PubMed
  48. Han Y, et al. 2020. Nat Commun. 11:1776. PubMed
  49. Yuzhu Hou et al. 2018. Immunity. 49(3):490-503 . PubMed
  50. Chen J, et al. 2022. J Nanobiotechnology. 20:283. PubMed
  51. Achmus L, et al. 2020. Front Neurol. 11:577971. PubMed
  52. Burrack KS et al. 2018. Immunity. 48(4):760-772 . PubMed
  53. Yoon Y, et al. 2021. Cancers (Basel). 13: . PubMed
  54. Cai B, et al. 2021. Mol Cancer. 20:165. PubMed
  55. Yang F, et al. 2020. Cancer Res. 80:3677. PubMed
  56. Fachi JL, et al. 2020. J Exp Med. 217:00:00. PubMed
  57. Jin Q, et al. 2022. Drug Des Devel Ther. 16:3297. PubMed
  58. Khan IM, et al. 2021. Immunohorizons. 5:703. PubMed
  59. Zirngibl F, et al. 2021. J Immunother Cancer. 9:. PubMed
  60. Yang K, et al. 2022. J Clin Invest. 132:. PubMed
  61. Wilson AS, et al. 2022. Nat Commun. 13:528. PubMed
  62. Fulham MA, et al. 2019. Am J Physiol Cell Physiol. 317:C687. PubMed
  63. Domingos-Pereira S, et al. 2019. Cancer Immunol Res. 7:621. PubMed
  64. Hurrell BP, et al. 2019. Cell Rep. 29:4509. PubMed
  65. Chen X et al. 2017. Cell stem cell. 21(6):747-760 . PubMed
  66. Chakraborty S, et al. 2022. Sci Transl Med. . PubMed
  67. Mamedov MR, et al. 2018. Immunity. 48:350. PubMed
  68. Soutto M, et al. 2017. J Cancer. 8:2424. PubMed
  69. Wang Y, et al. 2021. Front Immunol. 12:654463. PubMed
  70. Pishesha N, et al. 2021. Proc Natl Acad Sci U S A. 118:. PubMed
  71. Cheng B, et al. 2022. Cancer Commun (Lond). 42:17. PubMed
  72. Vidy A, et al. 2016. PLoS One. 11:e0165361. PubMed
  73. Vettorazzi S, et al. 2015. Nat Commun. 6: 7796. PubMed
  74. Habib S, et al. 2018. Infect Immun. 86:e00019. PubMed
  75. Kennedy EM, et al. 2022. Nat Commun. 13:5907. PubMed
  76. Ercoli G, et al. 2022. Clin Transl Immunology. 11:e1366. PubMed
  77. Cai Y, et al. 2019. Cell Rep. 27:3034. PubMed
  78. Fu Z, et al. 2021. Cell Res. 31:631. PubMed
  79. Colombo M, et al. 2022. iScience. 25:105042. PubMed
  80. Chatterjee D, et al. 2021. Cell Reports. 35(2):108996. PubMed
  81. Ballesteros I, et al. 2020. Cell. 183(5):1282-1297.e18. PubMed
  82. Tian Y, et al. 2016. J Immunol. 197: 1308 - 1321. PubMed
  83. Ren Y, et al. 2015. Sci Rep. 5: 18099. PubMed
  84. Andrade ACDSP, et al. 2021. J Virol. 95:e0127621. PubMed
  85. Shafiei-Jahani P, et al. 2021. Nat Commun. 12:2526. PubMed
  86. Wei Z, et al. 2021. Nat Commun. 0.805555556. PubMed
  87. Koelwyn GJ, et al. 2020. Nat Med. 1452:26. PubMed
  88. Agarwal P, et al. 2019. Cell Stem Cell. 24:769. PubMed
  89. Niderla-Bielinska J, et al. 2021. Int J Mol Sci. :22. PubMed
  90. Qadir AS, et al. 2021. iScience. 24:103348. PubMed
  91. Zhou J, et al. 2022. Int J Oncol. 61: . PubMed
  92. Al-Barwani F, et al. 2014. PLoS One. 9:104523. PubMed
  93. Varikuti S, et al. 2020. Br J Cancer. 122:1005. PubMed
  94. Bennett FC, et al. 2018. Neuron. 98:1170. PubMed
  95. Shehata L, et al. 2019. Cell Rep. 28:3300. PubMed
  96. Karanika S, et al. 2022. Front Immunol. 13:972266. PubMed
  97. Si J, et al. 2020. Cancer Cell. 38(4):551-566.e11. PubMed
  98. Laubreton D, et al. 2020. Viruses. 12:00. PubMed
  99. Mairhofer D, et al. 2015. J Invest Dermatol. 135: 2785-93. PubMed
  100. Ma Q, et al. 2022. Vaccines (Basel). 10:. PubMed
  101. Reda M, et al. 2022. Nat Commun. 13:4261. PubMed
  102. Zheng Y, et al. 2022. J Immunol. 208:501. PubMed
  103. Huang WC, et al. 2020. Adv Mater. 32:e2005637. PubMed
  104. Di Pilato M, et al. 2021. Cell. 184(17):4512-4530.e22. PubMed
  105. Knox T, et al. 2019. Sci Rep. 9:6136. PubMed
  106. Theurich S et al. 2017. Cell metabolism. 26(1):171-184 . PubMed
  107. Jee JJ, et al. 2022. Nat Commun. 13:18. PubMed
  108. Kim S, et al. 2022. Cancers (Basel). 14:. PubMed
  109. Tähtinen S, et al. 2022. Nat Immunol. 23:532. PubMed
  110. Ukidve A, et al. 2020. Proc Natl Acad Sci U S A. 117:17727. PubMed
  111. Jiang Y, et al. 2015. MBio. 6: e01426-15. PubMed
  112. Sun Y, et al. 2022. iScience. 25:104846. PubMed
  113. Agarwal P, et al. 2021. Cell Reports. 36(2):109386. PubMed
  114. Konishi Y, et al. 2018. iScience. 10:98. PubMed
  115. Lai NY, et al. 2020. Cell. 180:33:00. PubMed
  116. Chirivi RGS, et al. 2020. Cell Mol Immunol. . PubMed
  117. Pessoa Rodrigues C, et al. 2020. Sci Adv. 6:eaaz4815. PubMed
  118. Tan DQ, et al. 2019. Cell Rep. 26:2316. PubMed
  119. Riopel M, et al. 2019. Mol Metab. 20:89. PubMed
  120. Song X, et al. 2022. Transl Oncol. 15:101306. PubMed
RRID
AB_1595492 (BioLegend Cat. No. 100217)
AB_1595492 (BioLegend Cat. No. 100218)

Antigen Details

Structure
Ig superfamily, CD3/TCR, 20 kD
Distribution

Thymocytes (differentiation dependent), mature T cells, NK-T cells

Function
Antigen recognition, TCR signal transduction, T cell activation
Ligand/Receptor
Peptide antigen/MHC-complex
Antigen References

1. Barclay A, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Davis MM. 1990. Annu. Rev. Biochem. 59:475.
3. Weiss A, et al. 1994. Cell 76:263.

Gene ID
12502 View all products for this Gene ID
Specificity (DOES NOT SHOW ON TDS):
CD3
Specificity Alt (DOES NOT SHOW ON TDS):
CD3
App Abbreviation (DOES NOT SHOW ON TDS):
FC
UniProt
View information about CD3 on UniProt.org

Related FAQs

How stable is PerCP/Cyanine5.5 tandem as compared to PerCP alone?

PerCP/Cyanine5.5 is quite photostable and also better than PerCP alone in withstanding fixation.

Other Formats

View All CD3 Reagents Request Custom Conjugation
Description Clone Applications
FITC anti-mouse CD3 17A2 FC
PE anti-mouse CD3 17A2 FC
Purified anti-mouse CD3 17A2 FC,IHC-F,IP,ICC
Alexa Fluor® 647 anti-mouse CD3 17A2 FC,IHC-F,3D IHC,SB
Alexa Fluor® 488 anti-mouse CD3 17A2 FC,IHC-F,3D IHC
Pacific Blue™ anti-mouse CD3 17A2 FC
Alexa Fluor® 700 anti-mouse CD3 17A2 FC
PerCP/Cyanine5.5 anti-mouse CD3 17A2 FC
PE/Cyanine7 anti-mouse CD3 17A2 FC
APC/Cyanine7 anti-mouse CD3 17A2 FC
Brilliant Violet 421™ anti-mouse CD3 17A2 FC,ICC
Brilliant Violet 570™ anti-mouse CD3 17A2 FC
Brilliant Violet 650™ anti-mouse CD3 17A2 FC
Brilliant Violet 785™ anti-mouse CD3 17A2 FC
Brilliant Violet 510™ anti-mouse CD3 17A2 FC
APC anti-mouse CD3 17A2 FC
Ultra-LEAF™ Purified anti-mouse CD3 17A2 FC,IHC-F,IP,ICC
Brilliant Violet 605™ anti-mouse CD3 17A2 FC
Alexa Fluor® 594 anti-mouse CD3 17A2 IHC-F,FC,3D IHC,SB
Brilliant Violet 711™ anti-mouse CD3 17A2 FC
Biotin anti-mouse CD3 17A2 FC,IHC-F
PE/Dazzle™ 594 anti-mouse CD3 17A2 FC
APC/Fire™ 750 anti-mouse CD3 17A2 FC
Brilliant Violet 750™ anti-mouse CD3 17A2 FC
TotalSeq™-A0182 anti-mouse CD3 17A2 PG
TotalSeq™-B0182 anti-mouse CD3 17A2 PG
Spark Blue™ 550 anti-mouse CD3 17A2 FC
Spark NIR™ 685 anti-mouse CD3 17A2 FC
TotalSeq™-C0182 anti-mouse CD3 17A2 PG
APC/Fire™ 810 anti-mouse CD3 17A2 FC
PE/Fire™ 640 anti-mouse CD3 17A2 FC
Spark YG™ 570 anti-mouse CD3 17A2 IHC-F
PE/Fire™ 700 anti-mouse CD3 17A2 FC
PE/Cyanine5 anti-mouse CD3 17A2 FC
Spark Blue™ 574 anti-mouse CD3 Antibody 17A2 FC
Spark Violet™ 423 anti-mouse CD3 17A2 FC
PE/Fire™ 810 anti-mouse CD3 17A2 FC
Spark Red™ 718 anti-mouse CD3 17A2 FC
Spark UV™ 387 anti-mouse CD3 17A2 FC
Go To Top Version: 3    Revision Date: 02.08.2019

For Research Use Only. Not for diagnostic or therapeutic use.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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