- Clone
- QA16A08 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- Fusin, LESTR, Sdf1r, Cmkar4, PB-CKR, PBSF/SDF-1
- Isotype
- Mouse IgG1, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
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CD184, also known as CXCR4, is a member of the G protein coupled receptor family that binds CXCL12 (SDF1). CXCR4 and CXCL12 play an important role in immune and inflammatory responses through the regulation of cell migration and growth. CXCR4 plays a crucial role in the pathogenesis of several autoimmune diseases such as atherosclerosis, rheumatoid arthritis, and wound healing. In addition, CXCR4 is the cofactor for fusion and entry of the T cell-tropic form of HIV-1.
Product DetailsProduct Details
- Verified Reactivity
- Mouse
- Antibody Type
- Recombinant
- Host Species
- Mouse
- Immunogen
- Mouse CXCR4-transfected cells
- Formulation
- 0.2 µm filtered in phosphate-buffered solution, pH 7.2, containing no preservative. Endotoxin level is <0.01 EU/µg of the protein (<0.001 ng/µg of the protein) as determined by the LAL test.
- Preparation
- The Ultra-LEAF™ (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.
- Concentration
- The antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL. Older lots may have also been bottled at 1 mg/mL. Please contact technical support for concentration and total µg amount, or use our Lookup tool if you have a lot number.
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF™ solution contains no preservative; handle under aseptic conditions.
- Application
-
FC - Quality tested
- Recommended Usage
-
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 1 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
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This recombinant antibody clone is derived from clone L276F12.
Antigen Details
- Structure
- G-protein-coupled seven transmembrane receptor, 39.7 kD
- Distribution
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T lymphocytes, monocytes, macrophages, tissue-committed stem/progenitor cells (TCSCs), mast cells, vascular smooth muscle cells (VSMCs)
- Function
- Development of cardiovascular and central nervous system, hematopoiesis, and colonization of BM by fetal liver-derived hematopoietic stem cells (HSCs) during embryogenesis, homing and egress of CD34+ CXCR4+ progenitor cells from bone marrow, and their migration into peripheral tissues. Critical role in homing of cancer cells to specific metastatic sites. Possible role in the ubiquitin proteosome system.
- Interaction
- Robo -1
- Ligand/Receptor
- CXCL12 (SDF-1)
- Cell Type
- Macrophages, Mast cells, Monocytes, T cells
- Biology Area
- Cell Biology, Immunology, Neuroscience, Neuroscience Cell Markers
- Molecular Family
- CD Molecules, Cytokine/Chemokine Receptors, GPCR
- Antigen References
-
- McDermott DH, et al. 2015. Cell. 160:686
- Beck TC, et al. 2014. J Exp Med. 211:2567
- Ding BS, et al. 2014. Nature. 505:97
- Balabanian K, et al. 2012. Blood. 119:5722
- Gene ID
- 12767 View all products for this Gene ID
- Specificity (DOES NOT SHOW ON TDS):
- CD184
- Specificity Alt (DOES NOT SHOW ON TDS):
- CD184 (CXCR4)
- App Abbreviation (DOES NOT SHOW ON TDS):
- FC
- UniProt
- View information about CD184 on UniProt.org
Related Pages & Pathways
Pages
Related FAQs
- Do you guarantee that your antibodies are totally pathogen free?
-
BioLegend does not test for pathogens in-house aside from the GoInVivo™ product line. However, upon request, this can be tested on a custom basis with an outside, independent laboratory.
- Does BioLegend test each Ultra-LEAF™ antibody by functional assay?
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No, BioLegend does not test Ultra-LEAF™ antibodies by functional assays unless otherwise indicated. Due to the possible complexities and variations of uses of biofunctional antibodies in different assays and because of the large product portfolio, BioLegend does not currently perform functional assays as a routine QC for the antibodies. However, we do provide references in which the antibodies were used for functional assays and we do perform QC to verify the specificity and quality of the antibody based on our strict specification criteria.
- Does BioLegend test each Ultra-LEAF™ antibody for potential pathogens?
-
No, BioLegend does not test for pathogens in-house unless otherwise indicated. However, we can recommend an outside vendor to perform this testing as needed.
- Have you tested this Ultra-LEAF™ antibody for in vivo or in vitro applications?
-
We don't test our antibodies for in vivo or in vitro applications unless otherwise indicated. Depending on the product, the TDS may describe literature supporting usage of a particular product for bioassay. It may be best to further consult the literature to find clone specific information.
Other Formats
View All CD184 (CXCR4) Reagents Request Custom ConjugationDescription | Clone | Applications |
---|---|---|
PE anti-mouse CD184 (CXCR4) Recombinant Antibody | QA16A08 | FC |
Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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PE anti-mouse CD184 (CXCR4) Recombinant Antibody
C57BL/6 mouse thymocytes were stained with CD4 APC and CXCR4...
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