Loading...

FITC anti-human CD3 Antibody

Pricing & Availability
Clone
UCHT1 (See other available formats)
Regulatory Status
RUO
Workshop
III 471
Other Names
T3, CD3ε
Isotype
Mouse IgG1, κ
Ave. Rating
Submit a Review
Product Citations
publications
UCHT1_FITC_021805
Human peripheral blood lymphocytes stained with UCHT1 FITC
  • UCHT1_FITC_021805
    Human peripheral blood lymphocytes stained with UCHT1 FITC
Compare all formats See FITC spectral data
Cat # Size Price Quantity Check Availability Save
300405 25 tests 18€
Check Availability


Need larger quantities of this item?
Request Bulk Quote
300406 100 tests 40€
Check Availability


Need larger quantities of this item?
Request Bulk Quote
300452 100 µg 44€
Check Availability


Need larger quantities of this item?
Request Bulk Quote
300440 500 tests 164€
Check Availability


Need larger quantities of this item?
Request Bulk Quote
Description

CD3ε is a 20 kD chain of the CD3/T-cell receptor (TCR) complex which is composed of two CD3ε, one CD3γ, one CD3δ, one CD3ζ (CD247), and a T-cell receptor (α/β or γ/δ) heterodimer. It is found on all mature T cells, NKT cells, and some thymocytes. CD3, also known as T3, is a member of the immunoglobulin superfamily that plays a role in antigen recognition, signal transduction, and T cell activation.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Human
Reported Reactivity
Chimpanzee
Antibody Type
Monoclonal
Host Species
Mouse
Formulation
µg size: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
test sizes: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).
Preparation
The antibody was purified by affinity chromatography, and conjugated with FITC under optimal conditions.
Concentration
µg sizes: 0.5 mg/mL
test sizes: lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining using the µg size, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application. For flow cytometric staining using the test sizes, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

Excitation Laser
Blue Laser (488 nm)
Application Notes

Additional reported applications (for the relevant formats) include: immunohistochemical staining of acetone-fixed frozen sections4,6,7 and formalin-fixed paraffin-embedded sections11, immunoprecipitation1, activation of T cells2,3,5, Western blotting9, and spatial biology (IBEX)16,17. The LEAF™ purified antibody (Endotoxin < 0.1 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 300413, 300414, and 300432). For highly sensitive assays, we recommend Ultra-LEAF™ purified antibody (Cat. No. 300437, 300438, 300465, 300466, 300473, 300474) with a lower endotoxin limit than standard LEAF™ purified antibodies (Endotoxin < 0.01 EU/µg).

Application References

(PubMed link indicates BioLegend citation)
  1. Salmeron A, et al. 1991. J. Immunol. 147:3047. (IP)
  2. Graves J, et al. 1991. J. Immunol. 146:2102. (Activ)
  3. Lafont V, et al. 2000. J. Biol. Chem. 275:19282. (Activ)
  4. Ryschich E, et al. 2003. Tissue Antigens 62:48. (IHC)
  5. Thompson AG, et al. 2004. J. Immunol. 173:1671. (Activ)
  6. Sakkas LI, et al. 1998. Clin. Diagn. Lab. Immun. 5:430. (IHC)
  7. Mack CL, et al. 2004. Pediatr. Res. 56:79. (IHC)
  8. Thakral D, et al. 2008. J. Immunol. 180:7431. (FC) PubMed
  9. Van Dongen JJM, et al. 1988. Blood 71:603. (WB)
  10. Yoshino N, et al. 2000. Exp. Anim. (Tokyo) 49:97. (FC)
  11. Pollard, K. et al. 1987. J. Histochem. Cytochem. 35:1329. (IHC)
  12. Luckashenak N, et al. 2013. J. Immunol. 190:27. PubMed
  13. Laurent AJ, et al. 2014. PLoS One. 9:103683. PubMed
  14. Li J, et al. 2015. Cancer Res. 75:508. PubMed
  15. Stoeckius M, et al. 2017. Nat. Methods. 14:865-868. (PG)
  16. Radtke AJ, et al. 2020. Proc Natl Acad Sci USA. 117:33455-33465. (SB) PubMed
  17. Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed
Product Citations
  1. Johnson DC, et al. 2018. Nat Med. 24:1151. PubMed
  2. Sajadi MM, et al. 2018. Cell. 173:1783. PubMed
  3. Jiang G, et al. 2020. Aging (Albany NY). 12:11466. PubMed
  4. Bhattacharya S, et al. 2020. Front Bioeng Biotechnol. 1.055555556. PubMed
  5. Soday L, et al. 2021. Frontiers in Immunology. 12:600056. PubMed
  6. Lin L, et al. 2020. NAR Genom Bioinform. 2:lqaa059. PubMed
  7. Carre C, et al. 2021. iScience. 24:102970. PubMed
  8. Qin L, et al. 2022. Sci Adv. 8:eabn3774. PubMed
  9. Gan X, et al. 2022. Proc Natl Acad Sci U S A. 119:e2200879119. PubMed
  10. Sušac L, et al. 2022. Cell. 185:3201. PubMed
  11. Saber MM, et al. 2022. J Immunol Res. 2022:7219207. PubMed
  12. McEvoy CM, et al. 2022. Nat Commun. 13:7634. PubMed
  13. Graciliano NG, et al. 2023. Front Immunol. 13:1031248. PubMed
  14. Sun W, et al. 2023. Nature. 614:309. PubMed
  15. Namvar N, et al. 2022. Iran J Allergy Asthma Immunol. 21:600. PubMed
  16. Zhong J, et al. 2023. Front Immunol. 13:1081889. PubMed
  17. Yen M, et al. 2022. Cell. 185:1414. PubMed
  18. Cao B, et al. 2022. Nat Commun. 13:6203. PubMed
  19. Shang P, et al. 2022. Acta Biochim Biophys Sin (Shanghai). 54:1497. PubMed
  20. Porbahaie M, et al. 2023. PLoS One. 18:e0279626. PubMed
  21. Li H, et al. 2023. Clin Rheumatol. 42:1327. PubMed
  22. Freund EC, et al. 2023. Methods Mol Biol. 2618:201. PubMed
  23. Gao F, et al. 2023. Immunity. 56:864. PubMed
  24. Lütge M, et al. 2023. Nat Immunol. . PubMed
  25. Imai H, et al. 2023. iScience. 26:106822. PubMed
  26. Patskovsky Y, et al. 2023. Nat Commun. 14:3763. PubMed
  27. Netsrithong R, et al. 2020. Stem Cell Research. 39:101487.. PubMed
  28. Rendeiro AF, et al. 2020. Nat Commun. 11:577. PubMed
  29. Ryzhov S, et al. 2019. J Am Heart Assoc. 8:e010874. PubMed
  30. Barrow AD et al. 2018. Cell. 172(3):534-548 . PubMed
  31. Ye Y, et al. 2022. Nat Commun. 13:6458. PubMed
  32. Li C, et al. 2022. J Exp Med. 219:. PubMed
  33. Klemm F, et al. 2020. Cell. 181(7):1643-1660.e17. PubMed
  34. Chen L, et al. 2018. Immunology. 155:123. PubMed
  35. Rolandelli A, et al. 2017. Sci Rep. 7:40666. PubMed
  36. Karlsson H, et al. 2015. PLoS One. 10: 0144787. PubMed
  37. Kimura I, et al. 2022. Cell Rep. 38:110218. PubMed
  38. Chen M, et al. 2021. Cancers (Basel). 13:. PubMed
  39. Laustsen A, et al. 2021. Elife. 10:. PubMed
  40. Pholcharee T, et al. 2021. Nat Commun. 12:1063. PubMed
  41. Eri Yamada et al. 2018. Cell host & microbe. 23(1):110-120 . PubMed
  42. Esaulova E, et al. 2020. Cell Host Microbe. 29(2):165-178.e8. PubMed
  43. Liu C, et al. 2021. Cell Res. 31:1106. PubMed
  44. Devalraju KP, et al. 2021. PLoS One. 16:e0257185. PubMed
  45. Ruetsche D, et al. 2022. Cells. 11:. PubMed
  46. Engelmann C, et al. 2017. J Transl Med. 10.1186/s12967-017-1288-3. PubMed
  47. Zhan Y, et al. 2021. JCI Insight. 6:. PubMed
  48. Jiang Y, et al. 2020. Clin Immunol. 218:108516. PubMed
  49. Bergamaschi L, et al. 2021. Immunity. 54(6):1257-1275.e8. PubMed
  50. Perelman SS, et al. 2021. Nat Microbiol. 6:731. PubMed
  51. Zirbes A, et al. 2021. J Mammary Gland Biol Neoplasia. 26:247. PubMed
  52. Ping Y, et al. 2020. Front Cell Dev Biol. 0.890972222. PubMed
  53. Maritz MF, et al. 2018. PLoS One. 13:e0196043. PubMed
  54. Shevyrev D, et al. 2021. Exp Ther Med. 209:21. PubMed
  55. Caduff N, et al. 2021. Cell Reports. 35(5):109056. PubMed
  56. Appios A, et al. 2021. Bio Protoc. 11:e4044. PubMed
  57. Yarmarkovich M, et al. 2021. Nature. 599:477. PubMed
  58. Yuan Z, et al. 2018. Emerg Microbes Infect. 7:59. PubMed
  59. Tu HA, et al. 2020. Cell Rep Med. 1:100155. PubMed
  60. Ramos RN, et al. 2022. Clin Transl Immunology. 11:e1392. PubMed
  61. Corleis B, et al. 2017. PLoS One. 12:e0173161. PubMed
  62. Ray RM, et al. 2019. Sci Rep. 9:4334. PubMed
  63. Laroni A, et al. 2016. J Autoimmun. 72:8-18. PubMed
  64. Rajamanickam V, et al. 2021. Cancer Immunol Res. 9:602. PubMed
  65. Sawasdee N, et al. 2022. Int J Mol Med. 49:. PubMed
  66. Mishra HK, et al. 2021. Front Immunol. 12:711621. PubMed
  67. Huang N, et al. 2020. Genome Biol. 1.03125. PubMed
  68. Del Alcazar D, et al. 2019. Cell Rep. 28:3047. PubMed
  69. Rossignol ED, et al. 2021. Cell Reports. 35(8):109167. PubMed
  70. Minervina AA, et al. 2022. Nat Immunol. 23:781. PubMed
  71. Cai D, et al. 2020. Cell Mol Immunol. 17:227. PubMed
  72. Krishnan S, et al. 2021. Clin Exp Immunol. 203:458. PubMed
  73. Coufal S, et al. 2019. Cells. 8. PubMed
  74. Wang J, et al. 2019. Oncol Lett. 4.948611111. PubMed
  75. Kariminia A, et al. 2016. Blood. 127: 3082 - 3091. PubMed
  76. Lima J, et al. 2016. Reprod Sci. 10.1177/1933719116653680. PubMed
  77. Meermeier E, et al. 2016. Nat Commun. 7:12506. PubMed
  78. Whitfield SJC, et al. 2017. J Immunol. 198:3989. PubMed
  79. Cizmeci D, et al. 2021. eLife. 10:00. PubMed
  80. Platten M, et al. 2021. Nature. 592:463. PubMed
  81. Jin S, et al. 2020. Cell Res. 30:950. PubMed
  82. Liu Y, et al. 2020. Methods Mol Biol. 2111:285. PubMed
  83. Bak S, et al. 2019. Front Immunol. 9:2953. PubMed
  84. Kato M, et al. 2021. PLoS One. 16:e0252116. PubMed
  85. Setliff I, et al. 2018. Cell Host Microbe. 23:845. PubMed
  86. Collins PL et al. 2018. Cell. 176(1-2):348-360 . PubMed
  87. Shin CH, et al. 2020. J Immunol. 205:3179. PubMed
  88. Hakki S, et al. 2022. Sci Rep. 12:1427. PubMed
  89. Cecotto L, et al. 2022. iScience. 25:105211. PubMed
  90. Chapelin F, et al. 2017. Sci Rep.. 10.1038/s41598-017-17669-4. PubMed
  91. Cantoni C, et al. 2021. Oncoimmunology. 1879530:10. PubMed
  92. Motozono C, et al. 2021. Cell Host Microbe. . PubMed
  93. Reuschl AK, et al. 2022. Cell Rep. 39:110650. PubMed
  94. Abe Y, et al. 2022. Nat Cell Biol. 24:565. PubMed
  95. Cipriano M, et al. 2022. Commun Biol. 5:52. PubMed
  96. Kaito Y, et al. 2022. Oncol Lett. 23:51. PubMed
  97. Wang Y, et al. 2021. Sci Adv. 7: . PubMed
  98. Tamai Y, et al. 2013. J Immunol. 190:4382. PubMed
  99. Nakatsugawa M, et al. 2015. J Immunol. 194 3487 . PubMed
  100. Nakatsugawa M, et al. 2016. Sci Rep. 6:23821. PubMed
  101. Duhen R, et al. 2021. Nat Commun. 12:1047. PubMed
  102. Wang Z, et al. 2021. Nat Commun. 12:1724. PubMed
  103. Capone S, et al. 2020. NPJ Vaccines. 5:94. PubMed
  104. Domanska D, et al. 2022. J Exp Med. 219:. PubMed
  105. van Erp EA, et al. 2020. Clin Transl Immunology. 9:e1112. PubMed
  106. Maas RR, et al. 2021. Nat Protoc. 16:4692. PubMed
  107. Suzuki D, et al. 2020. Stem Cell Reports. 14:49. PubMed
  108. Beyer AI, et al. 2017. Stem Cells Dev. 26:102. PubMed
  109. Kwok I, et al. 2020. Immunity. 53(2):303-318.e5. PubMed
  110. Chen YP, et al. 2020. Cell Res. 30:1024. PubMed
  111. Dyson MR, et al. 2020. MAbs. 12:1829335. PubMed
  112. Ochi T, et al. 2015. Cancer Immunol Res. 3: 1070-1081. PubMed
  113. Lee M, et al. 2022. Front Cell Dev Biol. 10:909557. PubMed
  114. Albrecht M, et al. 2022. Clin Transl Immunology. 11:e1398. PubMed
  115. Rambaldi B, et al. 2022. Haematologica. 107:2617. PubMed
  116. Vanoni G, et al. 2021. eLife. 10:00. PubMed
  117. Goc J, et al. 2021. Cell. 184:5015. PubMed
  118. Wang SQ, et al. 2021. J Viral Hepat. 28:795. PubMed
  119. Pappa V, et al. 2021. J Neuroimmunol. 353:577524. PubMed
  120. Bennstein SB, et al. 2020. eLife. 9:e55232.. PubMed
  121. Chang ZL, et al. 2020. Nature Protocols. 15(4):1507-1524.. PubMed
  122. Wang S, et al. 2022. Cancer Immunol Immunother. Online ahead of print. PubMed
  123. Schmidts A, et al. 2020. J Immunother Cancer. 8:. PubMed
RRID
AB_2562046 (BioLegend Cat. No. 300405)
AB_2562046 (BioLegend Cat. No. 300406)
AB_2562046 (BioLegend Cat. No. 300452)
AB_2562046 (BioLegend Cat. No. 300440)

Antigen Details

Structure
Ig superfamily, with the subunits of CD3γ, CD3δ, CD3ζ (CD247) and TCR (α/β or γ/δ) forms CD3/TCR complex, 20 kD
Distribution

Mature T and NK T cells, thymocyte differentiation

Function
Antigen recognition, signal transduction, T cell activation
Ligand/Receptor
Peptide antigen bound to MHC
Cell Type
NKT cells, T cells, Thymocytes, Tregs
Biology Area
Immunology, Innate Immunity
Molecular Family
CD Molecules, TCRs
Antigen References

1. Barclay N, et al. 1993. The Leucocyte FactsBook. Academic Press. San Diego.
2. Beverly P, et al. 1981. Eur. J. Immunol. 11:329.
3. Lanier L, et al. 1986. J. Immunol. 137:2501-2507.

Gene ID
916 View all products for this Gene ID
Specificity (DOES NOT SHOW ON TDS):
CD3
Specificity Alt (DOES NOT SHOW ON TDS):
CD3
App Abbreviation (DOES NOT SHOW ON TDS):
FC
UniProt
View information about CD3 on UniProt.org

Related FAQs

There are no FAQs for this product.

Other Formats

View All CD3 Reagents Request Custom Conjugation
Description Clone Applications
APC anti-human CD3 UCHT1 FC
Biotin anti-human CD3 UCHT1 FC
FITC anti-human CD3 UCHT1 FC
PE anti-human CD3 UCHT1 FC
PE/Cyanine5 anti-human CD3 UCHT1 FC
Purified anti-human CD3 UCHT1 FC,CyTOF®,IHC-F,IP,Activ,WB
Alexa Fluor® 647 anti-human CD3 UCHT1 FC,ICC,IHC-F
Alexa Fluor® 488 anti-human CD3 UCHT1 FC,ICC,IHC-F
Pacific Blue™ anti-human CD3 UCHT1 FC
PE/Cyanine7 anti-human CD3 UCHT1 FC
Alexa Fluor® 700 anti-human CD3 UCHT1 FC
APC/Cyanine7 anti-human CD3 UCHT1 FC
PerCP anti-human CD3 UCHT1 FC
PerCP/Cyanine5.5 anti-human CD3 UCHT1 FC
Brilliant Violet 421™ anti-human CD3 UCHT1 FC,ICC,IHC-F
Brilliant Violet 570™ anti-human CD3 UCHT1 FC
Ultra-LEAF™ Purified anti-human CD3 UCHT1 FC,CyTOF®,IHC-F,IP,Activ,WB
Purified anti-human CD3 (Maxpar® Ready) UCHT1 FC,CyTOF®
Alexa Fluor® 594 anti-human CD3 UCHT1 ICC,FC,SB
PE/Dazzle™ 594 anti-human CD3 UCHT1 FC
Brilliant Violet 510™ anti-human CD3 UCHT1 FC
Brilliant Violet 605™ anti-human CD3 UCHT1 FC
Brilliant Violet 711™ anti-human CD3 UCHT1 FC
Brilliant Violet 650™ anti-human CD3 UCHT1 FC
APC/Fire™ 750 anti-human CD3 UCHT1 FC
Pacific Blue™ anti-human CD3 UCHT1 FC
Brilliant Violet 785™ anti-human CD3 UCHT1 FC
PE/Dazzle™ 594 anti-human CD3 UCHT1 FC
TotalSeq™-A0034 anti-human CD3 UCHT1 PG
TotalSeq™-B0034 anti-human CD3 UCHT1 PG
TotalSeq™-C0034 anti-human CD3 UCHT1 PG
PE anti-human CD3 UCHT1 FC
PE/Cyanine7 anti-human CD3 UCHT1 FC
KIRAVIA Blue 520™ anti-human CD3 UCHT1 FC
Spark Violet™ 538 anti-human CD3 Antibody UCHT1 FC
TotalSeq™-D0034 anti-human CD3 UCHT1 PG
Spark Blue™ 574 anti-human CD3 Antibody UCHT1 FC
GMP Pacific Blue™ anti-human CD3 UCHT1 FC
GMP PE anti-human CD3 UCHT1 FC
GMP PE/Dazzle™ 594 anti-human CD3 UCHT1 FC
Spark Violet™ 423 anti-human CD3 UCHT1 FC
GMP PE/Cyanine7 anti-human CD3 UCHT1 FC
Spark Blue™ 515 anti-human CD3 UCHT1 FC
Go To Top Version: 1    Revision Date: 11.30.2012

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

ProductsHere
Login / Register
Remember me
Forgot your password? Reset password?
Create an Account