Loading...

PE/Cyanine7 anti-mouse Ly-6G Antibody

Pricing & Availability
Clone
1A8 (See other available formats)
Regulatory Status
RUO
Other Names
Lymphocyte antigen 6 complex, locus G
Isotype
Rat IgG2a, κ
Ave. Rating
Submit a Review
Product Citations
publications
1A8_PECy7_092409
C57BL/6 bone marrow cells stained with 1A8 PE/Cyanine7
  • 1A8_PECy7_092409
    C57BL/6 bone marrow cells stained with 1A8 PE/Cyanine7
Compare all formats See PE/Cyanine7 spectral data
Cat # Size Price Quantity Check Availability Save
127617 25 µg 80€
Check Availability


Need larger quantities of this item?
Request Bulk Quote
127618 100 µg 243€
Check Availability


Need larger quantities of this item?
Request Bulk Quote
Description

Lymphocyte antigen 6 complex, locus G (Ly-6G), a 21-25 kD GPI-anchored protein, is expressed on the majority of myeloid cells in bone marrow and peripheral granulocytes.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Ly-6G transfected EL-4J cell line.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with PE/Cyanine7 under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

While 1A8 recognizes only Ly-6G, clone RB6-8C5 recognizes both Ly-6G and Ly-6C. Clone RB6-8C5 binds with high affinity to mouse Ly-6G molecules and to a lower extent to Ly-6C15. Clone RB6-8C5 impairs the binding of anti-mouse Ly-6G clone 1A815. However, clone RB6-8C5 is able to stain in the presence of anti-mouse Ly-6C clone HK1.416.

Additional reported applications (for the relevant formats) include: immunohistochemistry9 of frozen sections10 and paraffin-embedded sections11, depletion4, 12-14, and spatial biology (IBEX)20,21. The Ultra-LEAF™ purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for in vivo studies or highly sensitive assays (Cat. No. 127632, 127649, 127650, 127661 and 127662).

Application References

(PubMed link indicates BioLegend citation)
  1. Fleming TJ, et al. 1993. J. Immunol. 151:2399. (FC)
  2. Daley JM, et al. 2008. J. Leukocyte Biol. 83:1. (FC)
  3. Dietlin TA, et al. 2007. J. Leukocyte Biol. 81:1205. (FC)
  4. Daley J, et al. 2007. J. Leukocyte Biol. doi:10.1189. (Deplete) PubMed
  5. Tadagavadi RK, et al. 2010. J. Immunol. 185:4904. PubMed
  6. Sumagin R, et al. 2010. J. Immunol. 185:7057. PubMed
  7. Guiducci C, et al. 2010. J. Exp Med. 207:2931. PubMed
  8. Fujita M, et al. 2011. Cancer Res. 71:2664. PubMed
  9. Van Leeuwen, et al. 2008. Arterioscler. Thromb. Vasc. Biol. 28:84. (IHC)
  10. Kowanetz M, et al. 2010. P. Natl. Acad. Sci. USA 107:21248. [supplementary data] (IHC)
  11. Esbona K, et al. 2016. Breast Cancer Res. 18:35. (IHC)
  12. Wojtasiak M, et al. 2010. J. Gen. Virol. 91:2158. (FC, Deplete)
  13. Jaeger BN, et al. 2012. J. Exp. Med. 209:565. (Deplete)
  14. Wozniak KL, et al. 2012. BMC Immunol. 13:65 (FC, Deplete)
  15. Ribechini E, et al. 2009. Eur. J. Immunol. 39:3538.
  16. Ng LG, et al. 2011. J Invest. Dermatol. 131:2058. PubMed
  17. Ma C, et al. 2012. J. Leukoc. Biol. 92:1199.
  18. McCartney-Francis, N, et al. 2014. J Leukoc. Biol. 96:917. PubMed
  19. Her Z, et al. 2014. EMBO Mol. Med. 7:24. PubMed
  20. Radtke AJ, et al. 2020. Proc Natl Acad Sci U S A. 117:33455-65. (SB) PubMed
  21. Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed
Product Citations
  1. Laura C Burzynski et al. 2019. Immunity. 50(4):1033-1042 . PubMed
  2. Furuya Y, et al. 2014. J Virol. 88:9166. PubMed
  3. Dudek M, et al. 2015. Mucosal Immunol. . PubMed
  4. Lin C, et al. 2020. Cancer Immunol Res. 632:8. PubMed
  5. Argüello RJ, et al. 2020. Cell Metab. 32:1063. PubMed
  6. Bernasconi R, et al. 2021. EMBO Mol Med. :e14392. PubMed
  7. Buckland RL, et al. 2020. Curr Protoc Mouse Biol. 10:e78. PubMed
  8. Zhang Y, et al. 2022. J Cereb Blood Flow Metab. 42:952. PubMed
  9. Alam A, et al. 2022. Cancer Cell. 40:153. PubMed
  10. Martin-Martin I, et al. 2022. Cell Rep. 39:110648. PubMed
  11. Liu H, et al. 2022. Cell Rep Med. 3:100660. PubMed
  12. Miró L, et al. 2022. Cancers (Basel). 14: . PubMed
  13. Ullah I, et al. 2023. Cell Rep Med. 4:100893. PubMed
  14. Domingos-Pereira S, et al. 2022. Int J Mol Sci. 24: . PubMed
  15. Fan R, et al. 2023. J Immunother Cancer. 11: . PubMed
  16. Kulkarni R, et al. 2023. Life Sci Alliance. 6: . PubMed
  17. Li XF, et al. 2023. J Exp Med. 220: . PubMed
  18. Kang J, et al. 2023. JCI Insight. 8: . PubMed
  19. Schick J, et al. 2023. Elife. 12: . PubMed
  20. Hu R, et al. 2023. Cell Death Discov. 9:50. PubMed
  21. Olofsen PA, et al. 2022. Cells. 11:. PubMed
  22. Solier S, et al. 2023. Nature. 617:386. PubMed
  23. Vereertbrugghen A, et al. 2023. J Neuroinflammation. 20:120. PubMed
  24. Wang Z, et al. 2023. Mediators Inflamm. 2023:9810733. PubMed
  25. Zhao C, et al. 2023. NPJ Regen Med. 8:29. PubMed
  26. Huang T, et al. 2023. Cell Death Dis. 14:354. PubMed
  27. Frisbee AL, et al. 2019. Nat Commun. 10:2712. PubMed
  28. Israelow B, et al. 2020. bioRxiv. . PubMed
  29. Bieren J, et al. 2015. J Immunol. 194:1154. PubMed
  30. Gawish R, et al. 2022. Elife. 11:. PubMed
  31. Buxadé M, et al. 2018. J Exp Med. 215:2901. PubMed
  32. Kim TJ, et al. 2019. Nat Commun. 10:3258. PubMed
  33. Saleh MM, et al. 2019. Cell Host Microbe. 25:756. PubMed
  34. Lin R, et al. 2020. Sci Rep. 10:14397. PubMed
  35. Zasłona Z, et al. 2014. J Immunol. 193:4245. PubMed
  36. Mylonas K, et al. 2015. Immunobiology. 220: 924-933. PubMed
  37. Uzhachenko RV, et al. 2021. Cell Reports. 35(1):108944. PubMed
  38. Hewitson JP, et al. 2020. J Immunol. 204:2949. PubMed
  39. Hu W, et al. 2021. Nat Immunol. 22:1163. PubMed
  40. Mercer HL, et al. 2020. PLoS Pathog. 16:e1008572. PubMed
  41. Katsura A, et al. 2017. Mol Oncol. 11:1241. PubMed
  42. Lechner AJ et al. 2017. Cell stem cell. 21(1):120-134 . PubMed
  43. Zhao J, et al. 2019. Nat Commun. 10:899. PubMed
  44. Eastman A, et al. 2016. J Immunol . 194: 5999-6010. PubMed
  45. Douglas T, et al. 2015. J Immunol. 195: 2365-2373. PubMed
  46. Snyder LM, et al. 2021. PLoS Pathog. 17:e1009970. PubMed
  47. Liao Y, et al. 2017. Virol J. 10.1186/s12985-017-0801-x. PubMed
  48. Swidergall M, et al. 2019. Cell Rep. 28:423. PubMed
  49. Sanfilippo A, et al. 2015. Infect Immun . 83: 2976-2983. PubMed
  50. Starkl P, et al. 2020. Immunity. 53(4):793-804.e9. PubMed
  51. Zhu B, et al. 2021. Immunity. 54(6):1200-1218.e9. PubMed
  52. Jeong D, et al. 2021. eLife. 0.416666666666667. PubMed
  53. Jtte BB, et al. 2021. iScience. 24(8):102833. PubMed
  54. Molina-Sanchez P, et al. 2020. Gastroenterology. 2203:159. PubMed
  55. Gómez-Díaz C, et al. 2021. iScience. 24:103241. PubMed
  56. Boothby IC, et al. 2021. Nature. 599:667. PubMed
  57. Campana L, et al. 2018. J Immunol. 200:1169. PubMed
  58. Pan W et al. 2017. Immunity. 47(2):284-297 . PubMed
  59. Yu AI, et al. 2020. Cell Rep. 107471:31. PubMed
  60. Lu S, et al. 2020. JCI Insight. 5:00. PubMed
  61. Philip N, et al. 2016. PLoS Pathog. 12:e1005910. PubMed
  62. Park Y, et al. 2013. Cancer Res . 73:5669. PubMed
  63. Ma YV, et al. 2021. MAbs. 13:2003281. PubMed
  64. Sitnik S, et al. 2020. Mol Ther Oncolytics. 17:190. PubMed
  65. Riffelmacher T et al. 2017. Immunity. 47(3):466-480 . PubMed
  66. Davidson S, et al. 2020. Cell Reports. 31(7):107628. PubMed
  67. Gabbita S, et al. 2015. PLoS One. 10: e0137305. PubMed
  68. Jassinskaja M, et al. 2021. Cell Reports. 34(12):108894. PubMed
  69. Donlan AN, et al. 2020. Anaerobe. 66:102275. PubMed
  70. Yu X, et al. 2022. J Extracell Vesicles. 11:e12185. PubMed
  71. Dane EL, et al. 2022. Nat Mater. 21:710. PubMed
  72. Zhou Y, et al. 2022. Nat Commun. 13:2906. PubMed
  73. Domingos-Pereira S, et al. 2019. Cancer Immunol Res. 7:621. PubMed
  74. Liu D et al. 2019. Immunity. 51(1):64-76 . PubMed
  75. Singh S, et al. 2021. Cell Host Microbe. 29(3):347-361.e12. PubMed
  76. Yin S, et al. 2016. Nat Commun. 7: 11311. PubMed
  77. Chan C, et al. 2012. Hypertension. 60:1207. PubMed
  78. Ngamsri KC, et al. 2021. Int J Mol Sci. 22:. PubMed
  79. Guo L, et al. 2021. Front Immunol. 12:785457. PubMed
  80. Stump CT, et al. 2021. Open Biol. 11:210245. PubMed
  81. Sun KT, et al. 2018. Front Physiol. 1.06875. PubMed
  82. Shook BA, et al. 2020. Cell Stem Cell. 26(6):880-895. PubMed
  83. Galvani S, et al. 2015. Sci Signal. 8: ra79. PubMed
  84. Furuya Y, et al. 2015. PLoS Pathog. 11: 1005180. PubMed
  85. Lasso P, et al. 2020. Front Immunol. 584959:11. PubMed
  86. Grune J, et al. 2022. Nat Cardiovasc Res. 1:649. PubMed
  87. Guo P, et al. 2021. J Immunol. 207:408. PubMed
  88. Brown CC, et al. 2020. Cell. 179(4):846-863.e24.. PubMed
  89. Dmitrieva‐Posocco O et al. 2019. Immunity. 50(1):166-180 . PubMed
  90. Yadava K et al. 2019. Elife. 8 pii: e44821. PubMed
  91. Nguyen GT, et al. 2021. iScience. 24(8):102871. PubMed
  92. AM C, et al. 2015. Proc Natl Acad Sci U S A. 112 7557 . PubMed
  93. , et al. 2021. Eur J Immunol. 51:2708. PubMed
  94. Uddin MJ, et al. 2022. Mucosal Immunol. 15:165. PubMed
  95. Kaufmann E et al. 2018. Cell. 172(1-2):176-190 . PubMed
  96. Cheng Y, et al. 2019. Clin Cancer Res. 25:2621. PubMed
  97. Li Y, et al. 2020. Cell Reports. 27(10):2809-2816.e3.. PubMed
  98. Coquery C, et al. 2014. PLoS One. 9:102284. PubMed
  99. Soni C, et al. 2020. Immunity. 52(6):1022-1038.e7. PubMed
  100. Engler AE, et al. 2020. Cell Reports. 33(13):108553. PubMed
  101. Yang Y, et al. 2022. Elife. 11:. PubMed
  102. Germundson DL, et al. 2022. Front Allergy. 3:870513. PubMed
  103. Fischer KJ, et al. 2019. J Immunol. 202:2027. PubMed
  104. Lei J, et al. 2021. Theranostics. 4251:11. PubMed
  105. Liu X, et al. 2020. Cell Host Microbe. 28(5):683-698.e6. PubMed
  106. Bryant J, et al. 2014. J Immunol. 192:6092. PubMed
  107. Otvos B, et al. 2021. Clin Cancer Res. 27:2038. PubMed
  108. Poczobutt JM, et al. 2021. Am J Respir Cell Mol Biol. 64:629. PubMed
  109. Ding P, et al. 2022. Bone Res. 10:42. PubMed
  110. Almishri W, et al. 2015. J Hepatol. 63: 943-951. PubMed
  111. Nair S, et al. 2021. JCI Insight. 6:. PubMed
  112. Bravo-Caparrós I, et al. 2020. FASEB J. 34:5951. PubMed
  113. Lingegowda H, et al. 2022. Front Reprod Health. 3:726936. PubMed
  114. Jordan S, et al. 2020. Cell. 178(5):1102-1114.e17.. PubMed
  115. Ngamsri KC, et al. 2020. Front Immunol. 11:407. PubMed
  116. Chen F, et al. 2016. J Immunol. 196: 4390 - 4399. PubMed
  117. Moore J, et al. 2015. Am J Physiol Heart Circ Physiol. 309: 906-917. PubMed
  118. Johansson J, et al. 2013. J Neurosci. 33:16016. PubMed
  119. Murdock BJ, et al. 2021. JCI Insight. 6:. PubMed
  120. Evrard M et al. 2018. Immunity. 48(2):364-379 . PubMed
  121. Chhatbar C et al. 2018. Cell reports. 25(1):118-129 . PubMed
  122. Upadhya R, et al. 2016. MBio. 7: 00547-16. PubMed
  123. Alexander K, et al. 2016. Immunol Cell Biol. 95:7-16. PubMed
  124. Kwak T, et al. 2020. Cell Reports. 33(13):108571. PubMed
  125. Wong NR, et al. 2021. Immunity. 54:2072. PubMed
  126. Yang YS, et al. 2022. Nat Commun. 13:6175. PubMed
  127. Pfirschke C, et al. 2020. Cell Rep. 32:108164. PubMed
  128. Israelow B, et al. 2020. J Exp Med. 217:00:00. PubMed
  129. Li N, et al. 2022. Nat Commun. 13:7281. PubMed
  130. Wang X, et al. 2021. Cell. 184:5357. PubMed
  131. Xiao Y, et al. 2021. Cell. 184:6037. PubMed
  132. Casselli T, et al. 2021. PLoS Pathog. 17:e1009256. PubMed
  133. Nguyen GT, et al. 2020. eLife. 9:e56656.. PubMed
  134. Ling Y, et al. 2016. Pharmacol Res. S1043-6618(16)31329-9. PubMed
  135. Tang W, et al. 2021. Front Cell Dev Biol. 9:728057. PubMed
  136. Tang S, et al. 2022. Virol J. 19:32. PubMed
  137. Smith KJ, et al. 2022. PLoS Biol. 20:e3001554. PubMed
  138. Han P, et al. 2020. Sci Adv. 6:eaaz1580. PubMed
  139. Mogilenko DA et al. 2019. Cell. 177(5):1201-1216 . PubMed
  140. Braza MS et al. 2018. Immunity. 49(5):819-828 . PubMed
  141. White JP et al. 2018. Cell. 175(5):1198-1212 . PubMed
  142. Zukauskas A, et al. 2018. mSphere. 3:e00303. PubMed
  143. Kwok I, et al. 2020. Immunity. 53(2):303-318.e5. PubMed
  144. Martins R, et al. 2016. Nat Immunol. 17:1361-1372. PubMed
  145. Chavkin NW, et al. 2021. J Am Heart Assoc. 10:e019904. PubMed
  146. Wang X, et al. 2021. Theranostics. 11:209. PubMed
  147. Thurlow LR, et al. 2018. Cell Host Microbe. 24:261. PubMed
  148. Geng S, et al. 2019. Sci Adv. 5:eaav2309. PubMed
  149. Zhou J, et al. 2019. Cell Stem Cell. 24:285. PubMed
  150. Lee J, et al. 2015. Nat Commun. 6: 8499. PubMed
  151. Goc J, et al. 2021. Cell. 184:5015. PubMed
  152. Das A, et al. 2020. J Bone Miner Res. 36:199. PubMed
  153. Dietschmann A, et al. 2020. Eur J Immunol. 50:1044. PubMed
  154. Chirivi RGS, et al. 2020. Cell Mol Immunol. . PubMed
  155. Denk F, et al. 2016. Cell Rep. 15: 1771-1781. PubMed
  156. Wu L, et al. 2021. Cell Death Dis. 12:1064. PubMed
RRID
AB_1877261 (BioLegend Cat. No. 127617)
AB_1877261 (BioLegend Cat. No. 127618)

Antigen Details

Structure
A 21-35 kD GPI-anchorded membrane protein
Distribution

Expressed on the majority of myeloid cells in bone marrow and peripheral granulocytes. The monoclonal antibody RB6-8C5 recognizes both Ly-6G and Ly-6C.

Cell Type
Granulocytes, Macrophages, Monocytes
Biology Area
Immunology, Innate Immunity
Antigen References

Fleming TJ, et al. 1993. J. Immunol. 151:2399.

Gene ID
546644 View all products for this Gene ID
Specificity (DOES NOT SHOW ON TDS):
Ly-6G
Specificity Alt (DOES NOT SHOW ON TDS):
Ly-6G
App Abbreviation (DOES NOT SHOW ON TDS):
FC
UniProt
View information about Ly-6G on UniProt.org

Other Formats

View All Ly-6G Reagents Request Custom Conjugation
Description Clone Applications
Alexa Fluor® 594 anti-mouse Ly-6G 1A8 IHC-F,SB
Purified anti-mouse Ly-6G 1A8 FC,IHC-F,IHC-P,SB
Biotin anti-mouse Ly-6G 1A8 FC,IHC
FITC anti-mouse Ly-6G 1A8 FC
PE anti-mouse Ly-6G 1A8 FC
Alexa Fluor® 647 anti-mouse Ly-6G 1A8 FC,IHC-F,SB
Pacific Blue™ anti-mouse Ly-6G 1A8 FC
APC anti-mouse Ly-6G 1A8 FC
PerCP/Cyanine5.5 anti-mouse Ly-6G 1A8 FC
PE/Cyanine7 anti-mouse Ly-6G 1A8 FC
Alexa Fluor® 700 anti-mouse Ly-6G 1A8 FC,IHC
APC/Cyanine7 anti-mouse Ly-6G 1A8 FC
Alexa Fluor® 488 anti-mouse Ly-6G 1A8 FC,IHC-F,SB
Brilliant Violet 421™ anti-mouse Ly-6G 1A8 FC,IHC-F
Brilliant Violet 570™ anti-mouse Ly-6G 1A8 FC
Ultra-LEAF™ Purified anti-mouse Ly-6G 1A8 FC,Depletion,IHC
Brilliant Violet 510™ anti-mouse Ly-6G 1A8 FC
Purified anti-mouse Ly-6G (Maxpar® Ready) 1A8 FC,CyTOF®,WB
Brilliant Violet 650™ anti-mouse Ly-6G 1A8 FC
Brilliant Violet 711™ anti-mouse Ly-6G 1A8 FC
Brilliant Violet 605™ anti-mouse Ly-6G 1A8 FC
Brilliant Violet 785™ anti-mouse Ly-6G 1A8 FC
PE/Dazzle™ 594 anti-mouse Ly-6G 1A8 FC
APC/Fire™ 750 anti-mouse Ly-6G 1A8 FC
PerCP anti-mouse Ly-6G 1A8 FC
TotalSeq™-A0015 anti-mouse Ly-6G 1A8 PG
TotalSeq™-C0015 anti-mouse Ly-6G 1A8 PG
TotalSeq™-B0015 anti-mouse Ly-6G 1A8 PG
Spark Blue™ 550 anti-mouse Ly-6G 1A8 FC
Spark NIR™ 685 anti-mouse Ly-6G 1A8 FC
Spark YG™ 593 anti-mouse Ly-6G 1A8 FC
APC/Fire™ 810 anti-mouse Ly-6G Antibody 1A8 FC
PE/Cyanine5 anti-mouse Ly-6G 1A8 FC
PE/Fire™ 810 anti-mouse Ly-6G Antibody 1A8 FC
Spark UV™ 387 anti-mouse Ly-6G 1A8 FC
PE/Fire™ 640 anti-mouse Ly-6G 1A8 FC
Spark YG™ 570 anti-mouse Ly-6G 1A8 IHC-F,FC
Spark Red™ 718 anti-mouse Ly-6G (Flexi-Fluor™) 1A8 FC
Spark Blue™ 574 anti-mouse Ly-6G (Flexi-Fluor™) 1A8 FC
Go To Top Version: 3    Revision Date: 01.06.2017

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

ProductsHere
Login / Register
Remember me
Forgot your password? Reset password?
Create an Account