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- Clone
- 9E12C72 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- Senescence-associated epithelial membrane protein, CLDN1, CLD1, SEMP1,
- Isotype
- Mouse IgG1, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
-
Total cell lysates (15 µg protein) from 293T (negative control), A431 and NIH3T3 cells were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to nitrocellulose, and probed with 2 µg/mL purified anti-Claudin-1 (clone 9E12C72) antibody, competitor’s antibody used at manufactures recommended concentration (upper). Proteins were visualized by chemiluminescence detection using a goat anti-mouse-IgG secondary antibody conjugated to HRP for anti-Claudin-1 antibody, and a donkey anti-rabbit IgG Antibody conjugated to HRP for competitor’s antibody. Direct-Blot™ HRP anti-β-actin Antibody was used as a loading control (lower). Lane M: molecular weight ladder and M* indicates extended exposure of the molecular weight ladder.
| Cat # | Size | Price | Quantity Check Availability | Save | ||
|---|---|---|---|---|---|---|
| 697801 | 25 µg | 81€ | ||||
| 697802 | 100 µg | 203€ | ||||
Claudin-1 is a member of the claudin family of proteins, which is composed of at least 24 members. Claudin proteins are integral membrane proteins with 4 transmembrane regions and 2 extracellular loops that form tight junctions. Tight junctions function as a channel and a barrier to maintain cellular morphology, polarity, and homeostasis between epithelial cells. Claudin-1 is a main component of tight junctions and is sorely responsible for normal barrier function of the skin. Misregulated expression of Claudin-1 has been shown to be associated with cancer progression and metastasis in several cancers. Claudin-1 has been found to be overexpressed in neoplasia associated with inflammatory bowel disease. Mutations in Claudin-1 gene lead to human skin diseases and Claudin-1 deficient mice die soon after birth due to dehydration.
Product DetailsProduct Details
- Verified Reactivity
- Human
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- Synthetic Claudin-1 peptide conjugated to KLH (TPRPYPKPAPSSGKDYVC)
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography.
- Concentration
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C.
- Application
-
WB - Quality tested
- Recommended Usage
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Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.5 - 2.0 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
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This clone has no cross-reactivity with mouse (in house tested).
- RRID
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AB_2687191 (BioLegend Cat. No. 697801)
AB_2687191 (BioLegend Cat. No. 697802)
Antigen Details
- Structure
- 211 amino acids with a predicted molecular weight of 22.7 kD and observed approximately 18kD. Multi-pass membrane protein with 4 transmembrane domains.
- Distribution
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Cell membrane. Cell junction.
- Function
- Claudin-1 is an integral membrane protein that forms tight junctions between skin epithelial cells to form barriers, regulate paracellular transport, and maintain cell polarity.
- Interaction
- Forms homopolymers and heteropolymers with other claudin family proteins. Interacts with CLDN3, TJP1/ZO-1, TJP2/ZO-2, TJP3/ZO-3, MPDZ, and PATJ.
- Cell Type
- Mesenchymal Stem Cells
- Biology Area
- Stem Cells
- Antigen References
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1. Suh Y, et al. 2017. Oncogene. 36:1167.
2. Tokumasu R, et al. 2016. Proc. Natl. Acad. Sci. USA. 113:E4061.
3. Zhou B, et al. 2015. J. Clin. Med. 4:1960.
4. Saeedi BJ, et al. 2015. Mol. Biol. Cell. 26:2252.
5. Huang J, et al. 2015. Oncotarget. 6:1652.
6. Izraely S, et al. 2015. Int. J. Cancer. 136:1296. - Gene ID
- 9076 View all products for this Gene ID
- Specificity (DOES NOT SHOW ON TDS):
- Claudin-1
- Specificity Alt (DOES NOT SHOW ON TDS):
- Claudin-1
- App Abbreviation (DOES NOT SHOW ON TDS):
- WB
- UniProt
- View information about Claudin-1 on UniProt.org
Other Formats
View All Claudin-1 Reagents Request Custom Conjugation| Description | Clone | Applications |
|---|---|---|
| Purified anti-Claudin-1 | 9E12C72 | WB |
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Purified anti-Claudin-1
Total cell lysates (15 µg protein) from 293T (negative contr...
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