Alexa Fluor 488 anti-mouse CD107b Mac-3 Antibody anti-Mac-3

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Clone: M3/84 (See other available formats)
Regulatory Status: RUO
Other Names: CD107b, LAMP-2
Isotype: Rat IgG1, κ
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M3slash84_Alx488_011108 — Thioglycolate-elicited BALB/c mouse peritoneal macrophages stained with M3/84 Alexa Fluor® 488

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108510

100 µg

184€

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Description

Mac-3 is a 110 kD type I membrane glycoprotein, also known as CD107b and LAMP-2. It is expressed on lysosomal membranes and the plasma membrane of macrophages and some myeloid cell lines. In the bone marrow, few cells display Mac-3 antigen on the surface, but a large proportion express Mac-3 in the cytoplasm. CD107b has been identified as a ligand for galaptin, an S-type lectin present in the extracellular matrix. Mac-3/CD107b is upregulated in some tumors and increased expression has been correlated with enhanced metastatic potential.

Product Details

Technical data sheet

Product Details

Verified Reactivity: Mouse
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: Membrane glycoproteins from C57BL/6 mouse peritoneal exudate cells
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 488 under optimal conditions.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: FC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per 10⁶ cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor® 488 has a maximum emission of 519 nm when it is excited at 488 nm.

Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

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Excitation Laser: Blue Laser (488 nm)
Application Notes: Additional reported applications (for the relevant formats) include: immunoprecipitation^1-4 and immunohistochemical staining of acetone-fixed frozen sections^5,6and paraformaldehyde-fixed paraffin-embedded sections^9-11.
RRID: AB_493308 (BioLegend Cat. No. 108510)

Antigen Details

Structure: 110 kD
Distribution: Macrophages, some myeloid cell lines
Function: Differentiation and activation
Cell Type: Macrophages
Biology Area: Immunology, Innate Immunity
Molecular Family: CD Molecules
Antigen References: 1. Springer TA. 1981. J. Biol. Chem. 256:3833.
2. Ho MK, et al. 1983. J. Biol. Chem. 258:636.
3. Ralph P, et al. 1983. J. Immunol. 130:108.
Gene ID: 16784 View all products for this Gene ID
UniProt: View information about CD107b on UniProt.org

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Related Protocols

Cell Surface Flow Cytometry Staining Protocol

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Description	Clone	Applications

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Other Formats

View All CD107b Reagents Request Custom Conjugation

Description	Clone	Applications
FITC anti-mouse CD107b (Mac-3)	M3/84	FC
PE anti-mouse CD107b (Mac-3)	M3/84	FC
Purified anti-mouse CD107b (Mac-3)	M3/84	FC,IP,ICC,IHC-P,IHC-F
Biotin anti-mouse CD107b (Mac-3)	M3/84	FC
Alexa Fluor® 488 anti-mouse CD107b (Mac-3)	M3/84	FC
Alexa Fluor® 647 anti-mouse CD107b (Mac-3)	M3/84	FC,ICC

Certificate of Analysis

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Go To Top Version: 3 Revision Date: 06.27.2014

For Research Use Only. Not for diagnostic or therapeutic use.

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

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