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Alexa Fluor® 488 anti-mouse CD45.1 Antibody

Pricing & Availability
Clone
A20 (See other available formats)
Regulatory Status
RUO
Other Names
T200, Ly-5.1, LCA
Isotype
Mouse (A.SW) IgG2a, κ
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Product Citations
publications
A20_Alx488_090507
SJL mouse splenocytes stained with A20 Alexa Fluor® 488
  • A20_Alx488_090507
    SJL mouse splenocytes stained with A20 Alexa Fluor® 488
Compare all formats See Alexa Fluor® 488 spectral data
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110717 25 µg 81€
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110718 100 µg 184€
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Description

CD45.1 is an alloantigen of CD45, expressed by Ly5.1 bearing mouse strains (e.g., RIII, SJL/J, STS/A, DA). CD45, a member of the protein tyrosine phosphatase (PTP) family, is a 180-240 kD glycoprotein expressed on all hematopoietic cells except mature erythrocytes and platelets. There are multiple isoforms in mice that play key roles in TCR and BCR signal transduction. These isoforms are very specific to the activation and maturation states of the cell as well as specific cell types. The primary ligands for CD45 are galectin-1, CD2, CD3, CD4, TCR, CD22, and Thy-1.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
SJL mouse thymocytes and splenocytes
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 488 under optimal conditions.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per 106 cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor® 488 has a maximum emission of 519 nm when it is excited at 488 nm.


Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

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Excitation Laser
Blue Laser (488 nm)
Application Notes

The A20 antibody does not react with leukocytes or mouse cells expressing the CD45.2 alloantigen. Additional reported applications (for relevant formats of this clone) include: immunoprecipitation3, in vitro blocking of B cell responses1,2, immunohistochemical staining of frozen sections: OCT embedded7 and acetone-fixed4-6 (direct immunofluorescence detection with fluorochrome conjugated A20 was used in (5) and (6)).

Application References

(PubMed link indicates BioLegend citation)
  1. Yakura H, et al. 1983. J. Exp. Med. 157:1077. (Block)
  2. Yakura H, et al. 1986. J. Immunol. 136:2729. (Block)
  3. Shen FW, et al. 1986. Immunogenetics 24:146. (IP)
  4. Suzuki K, et al. 2000. Immunity 13:691. (IHC-F)
  5. Werner N, et al. 2002. Arterioscler. Thromb. Vasc. Biol. 22:1567. (IHC-F)
  6. Lessner SM, et al. 2002. Am. J. Pathol. 160:2145. (FC, IHC-F)
  7. Chen CC, et al. 2005. P. Natl. Acad. Sci. USA 102:11408 (IHC-F)
  8. Pascal V, et al. 2007. J. Immunol. 179:1751. (FC)
  9. Mende I, et al. 2006. Blood 107:1383. (IHC-F, FC)
  10. Phan TG, et al. 2007. Nature Immunol. 8:992. (FC)
  11. Wither DR, et al. 2009. J. Immunol. 183:5079. PubMed
  12. Pascal V, et al.2007. J. Immunol. 179:1751. PubMed
  13. Lee SW, et al. 2009. J. Immunol. 182:6753. PubMed
  14. Takada K, et al. 2009. J. Exp Med. 206:2253. PubMed
  15. Beamer CA, et al. 2007. Am. J. Respir. Cell. Mol. Biol. 37:729. (FC) PubMed
  16. Li LX, et al. 2010. J. Immunol. 184:1728. PubMed
  17. Hosoi A, et al. 2008. Cancer Res. 68:3941. (FC) PubMed
  18. Kenna TJ, et al. 2008. Blood 111:2091. PubMed
  19. Kohlmeier JE, et al. 2008. Immunity. 29:101. (FC) PubMed
Product Citations
  1. Enterina JR, et al. 2022. Cell Rep. 38:110512. PubMed
  2. Scherer S, et al. 2023. Nat Immunol. 24:501. PubMed
  3. Whyte CE, et al. 2022. Curr Protoc. 2:e589. PubMed
  4. Yazicioglu YF, et al. 2023. Nat Immunol. 24:991. PubMed
  5. Kaczanowska S, et al. 2021. Cell. 184(8):2033-2052.e21. PubMed
  6. Alam J, et al. 2022. Front Med (Lausanne). 9:849990. PubMed
  7. Gerber AN, et al. 2021. Cell Rep. 37:109816. PubMed
  8. Okuniewska M, et al. 2021. Cell Reports. 36(2):109368. PubMed
  9. Findlay EG, et al. 2019. Oncoimmunology. 8:1608106. PubMed
  10. Yang W, et al. 2020. Mucosal Immunol. 13:788. PubMed
  11. Fumagalli V, et al. 2020. J Exp Med. :217. PubMed
  12. El Amki M, et al. 2020. Cell Rep. 33:108260. PubMed
  13. Guo Z, et al. 2016. Nat Commun. 7:10307. PubMed
  14. Sharma S, et al. 2015. J Immunol. 194:5529. PubMed
  15. Emgård J, et al. 2018. Immunity. 143:419. PubMed
  16. Chopin M et al. 2018. Immunity. 50(1):77-90 . PubMed
  17. Alam J, et al. 2021. Front Immunol. 12:701415. PubMed
  18. Madenspacher JH, et al. 2020. JCI Insight. 5:00. PubMed
  19. Wu T, et al. 2014. J Leukoc Biol. 95:215. PubMed
  20. Pasciuto E, et al. 2020. Cell. 182:625. PubMed
  21. Tang Q, et al. 2021. Immunity. 54:1961. PubMed
  22. Hirasawa M, et al. 2016. J Biol Chem. 291: 7373-7385. PubMed
  23. Di Pilato M, et al. 2021. Cell. 184(17):4512-4530.e22. PubMed
  24. Allman W, et al. 2015. Proc Natl Acad Sci U S A. 112: 4094 - 4103. PubMed
RRID
AB_492862 (BioLegend Cat. No. 110717)
AB_492862 (BioLegend Cat. No. 110718)

Antigen Details

Structure
Protein tyrosine phosphatase (PTP) family, 180-240 kD
Distribution

All hematopoietic cells except mature erythrocytes and platelets of the CD45.1 strain of mice

Function
Phosphatase, T and B cell activation
Ligand/Receptor
Galectin-1, CD2, CD3, CD4
Biology Area
Cell Biology, Immunology, Inhibitory Molecules, Neuroscience, Neuroscience Cell Markers
Molecular Family
CD Molecules
Antigen References

1. Barclay A, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Trowbridge IS, et al. 1993. Annu. Rev. Immunol. 12:85.
3. Kishihara K, et al. 1993. Cell 74:143.
4. Pulido R, et al. 1988. J. Immunol. 140:3851.

Gene ID
19264 View all products for this Gene ID
UniProt
View information about CD45.1 on UniProt.org
Go To Top Version: 3    Revision Date: 11.19.2019

For Research Use Only. Not for diagnostic or therapeutic use.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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