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Alexa Fluor® 594 anti-human CD66d/e Antibody

Pricing & Availability
Clone
308/3-3 (See other available formats)
Regulatory Status
RUO
Other Names
CEACAM3, CGM1, CEACAM5, CEA
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
308slash3-3_A594A_CD66d-e_Antibody_IHC-P_1_042018
Human paraffin-embedded colon tissue slices were prepared with a standard protocol of deparaffinization and rehydration. Antigen retrieval was done with Citrate Buffered 1X (10mM, pH6.0) at 95°C for 40 minutes. Tissue was washed with PBS/0.05% Tween 20 twice for five minutes , permeabilized with PBS with 0.5% Triton X-100 for 15 mintues and blocked with 5% FBS and 0.2% gelatin for 30 minutes. Then, the tissue was stained with 10µg/mL of Alexa Fluor® 647 anti-Vimentin (clone O91D3) antibody (green) and 10µg/mL of Alexa Fluor® 594 anti-CD66d/e (clone 308/3-3) antibody (red) over night at 4°C. Nuclei were counterstained with DAPI (blue). The image was scanned with a 10X objective and stitched with MetaMorph® software.
  • 308slash3-3_A594A_CD66d-e_Antibody_IHC-P_1_042018
    Human paraffin-embedded colon tissue slices were prepared with a standard protocol of deparaffinization and rehydration. Antigen retrieval was done with Citrate Buffered 1X (10mM, pH6.0) at 95°C for 40 minutes. Tissue was washed with PBS/0.05% Tween 20 twice for five minutes , permeabilized with PBS with 0.5% Triton X-100 for 15 mintues and blocked with 5% FBS and 0.2% gelatin for 30 minutes. Then, the tissue was stained with 10µg/mL of Alexa Fluor® 647 anti-Vimentin (clone O91D3) antibody (green) and 10µg/mL of Alexa Fluor® 594 anti-CD66d/e (clone 308/3-3) antibody (red) over night at 4°C. Nuclei were counterstained with DAPI (blue). The image was scanned with a 10X objective and stitched with MetaMorph® software.
  • 308slash3-3_A594A_CD66d-e_Antibody_IHC-P_1_042018
    Human paraffin-embedded colon tissue slices were prepared with a standard protocol of deparaffinization and rehydration. Antigen retrieval was done with Citrate Buffered 1X (10mM, pH6.0) at 95°C for 40 minutes. Tissue was washed with PBS/0.05% Tween 20 twice for five minutes , permeabilized with PBS with 0.5% Triton X-100 for 15 mintues and blocked with 5% FBS and 0.2% gelatin for 30 minutes. Then, the tissue was stained with 10µg/mL of Alexa Fluor® 647 anti-Vimentin (clone O91D3) antibody (green) and 10µg/mL of Alexa Fluor® 594 anti-CD66d/e (clone 308/3-3) antibody (red) over night at 4°C. Nuclei were counterstained with DAPI (blue). The image was scanned with a 10X objective and stitched with MetaMorph® software.
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392803 25 µg 128€
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Description

The 308/3-3 mAb reacts with an antigen epitope shared by CD66d and e. CD66d/e are members of the CEA (carcinoembryonic antigen) family of the Ig superfamily. CD66 plays a role in hemophilic and heterophilic adhesion. CD66d is a major granulocyte receptor mediating recognition and efficient opsonin-independent phagocytosis of CEACAM-binding microorganisms, including Neisseria, Moraxella and Haemophilus species, thus playing an important role in the clearance of pathogens by the innate immune system. Responsible for RAC1 stimulation in the course of pathogen phagocytosis. CD66e molecule is also called CEA or CEACAM5, and is primarily found on epithelial cells. CD66e binds to CD66a, c and e.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Recombinant soluble human CEACAM3-Fc produced in HEK293 cells
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 594 under optimal conditions.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

IHC-P - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by formalin-fixed paraffin-embedded immunohistochemical staining. For immunohistochemistry, a concentration range of 5 - 10 µg/ml is suggested. It is recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor® 594 has an excitation maximum of 590 nm, and a maximum emission of 617 nm. 


Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

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RRID
AB_2734446 (BioLegend Cat. No. 392803)

Antigen Details

Structure
Ig superfamily, CEA antigen group, GPI-linked glycoprotein
Distribution

Granulocytes

Function
Cell adhesion, neutrophil activation
Interaction
CEACAM1, 5,6, SRC and ZPLD1.
Ligand/Receptor
CD66d: CEACAM1, SRC.
CD66e: CEACAM1, 5, 6.
Cell Type
Granulocytes
Biology Area
Immunology
Molecular Family
Adhesion Molecules, CD Molecules
Antigen References
  1. Chen T, et al. 2001. J Biol Chem. 276:17413
  2. Wang Z, et al. 2016. Mol Clin Oncol. 5:731
  3. Swords DS, et al. 2016. Onco Targets Ther. 9:7459
Gene ID
1084 View all products for this Gene ID
UniProt
View information about CD66d CD66e on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 05.10.2018

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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