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Alexa Fluor® 594 anti-mouse Galectin-9 Antibody

Pricing & Availability
Clone
108A2 (See other available formats)
Regulatory Status
RUO
Other Names
Lgals9, Gal-9, Ecalectin
Isotype
Rat IgG2a, κ
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Product Citations
publications
108A2_A594_Galectin-9_Antibody_IHC-F_102416
C57BL/6 mouse frozen testis section was fixed with 4% paraformaldehyde (PFA) for ten minutes at room temperature and blocked with 5% FBS plus 5% rat serum for one hour at room temperature. Then, the section was stained with 10 µg/ml of Galectin-9 (clone 108A2) Alexa Fluor® 594 (red) and 5 µg/mL of CD106 (clone 429 (MVCAM.A)) Alexa Fluor® 647 (green) overnight at 4°C. The image was captured with a 10X objective.
  • 108A2_A594_Galectin-9_Antibody_IHC-F_102416
    C57BL/6 mouse frozen testis section was fixed with 4% paraformaldehyde (PFA) for ten minutes at room temperature and blocked with 5% FBS plus 5% rat serum for one hour at room temperature. Then, the section was stained with 10 µg/ml of Galectin-9 (clone 108A2) Alexa Fluor® 594 (red) and 5 µg/mL of CD106 (clone 429 (MVCAM.A)) Alexa Fluor® 647 (green) overnight at 4°C. The image was captured with a 10X objective.
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137906 100 µg 296€
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Description

Galectin-9 is a mammalian lectin with a molecular weight of 40 kD that has two conserved carbohydrate recognition domains (CRDs) and forms homodimers. It recognizes N-acetyllactosamine (Galbeta1-4GlcNAc) and T-antigen (Galbeta1-3GalNAc); Tim-3 has been reported as its ligand. Galectin-9 is expressed by lymphocytes, dendritic cells, granulocytes, eosinophils, astrocytes, endothelial cells, fibroblasts and thymus epithelial cells. It may be retained intracellularly or be transported to the cell surface where it is cleaved, thereby generating a soluble form. Galectin-9 is involved in events such as cell aggregation, cell adhesion, chemotaxis and apoptosis. Importantly for the regulation of the immune response, Galectin-9 induces regulatory T cells and suppresses Th1 and Th17 responses.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Full length recombinant mouse Galectin-9 (M-type)
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 594 under optimal conditions.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

IHC-F - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunohistochemical staining on frozen tissue sections. For immunohistochemistry, a concentration range of 2.5 - 10 μg/ml is suggested. It is recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor® 594 has an excitation maximum of 590 nm, and a maximum emission of 617 nm.


Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

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Application Notes

Clone 108A2 recognizes the linker peptide of mouse Galectin-9; it does not cross-react with stable Galectin-9 (which is biologically active, genetically engineered, linker-less Galectin-9)3. Additional reported applications (for the relevant formats) include: sandwich ELISA as capture antibody1 and immunohistochemical staining of paraffin2 embedded tissue sections.

Application References

(PubMed link indicates BioLegend citation)
  1. Tsuboi Y, et al. 2007. Clin. Immunol. 124:221. (ELISA)
  2. Personal communication. (IHC-Paraffin)
  3. Oomizu S, et al. 2012. PLoS One. 7:e48574. PubMed
  4. Madireddi S, et al. 2014. J Exp Med. 211:1433. PubMed
Product Citations
  1. Chen HY, et al. 2020. J Immunol. 1158:204. PubMed
RRID
AB_2632697 (BioLegend Cat. No. 137906)

Antigen Details

Structure
Homodimer, each chain is a 343 aa protein of 40 kD that has two conserved carbohydrate recognition domains (CRDs)
Distribution

Lymphocytes, dendritic cells, neutrophils, eosinophils, astrocytes, endothelial cells, fibroblasts, thymus stromal/epithelial cells

Function
Cell aggregation, cell adhesion, chemotaxis, apoptosis, suppression of Th1 and Th17 responses, induction of regulatory T cells
Ligand/Receptor
Tim-3
Cell Type
Astrocytes, Dendritic cells, Endothelial cells, Eosinophils, Epithelial cells, Fibroblasts, Lymphocytes, Neutrophils, Tregs
Biology Area
Apoptosis/Tumor Suppressors/Cell Death, Cell Adhesion, Cell Biology, Cell Motility/Cytoskeleton/Structure, Immunology
Molecular Family
Adhesion Molecules, Immune Checkpoint Receptors
Antigen References

1. Klibi J, et al. 2009. Blood 113:1957.
2. Seki M, et al. 2008. Clin. Immunol. 127:78.
3. Tsuboi Y, et al. 2007. Clin. Immunol. 124:221.
4. Zhu C, et al. 2005. Nat. Immunol. 6:1245.
5. Dunphy JL, et al. 2002. J. Biol. Chem. 277:14916.

Gene ID
16859 View all products for this Gene ID
UniProt
View information about Galectin-9 on UniProt.org
Go To Top Version: 1    Revision Date: 10.24.2016

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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