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Alexa Fluor® 594 anti-T-bet Antibody

Pricing & Availability
Clone
4B10 (See other available formats)
Regulatory Status
RUO
Other Names
T-box expressed in T cells, T box 21, TBLYM
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
A_4B10_A594_T-bet_Antibody_ICFC_3_032724
PBMCs were surface stained with Alexa Fluor® 488 anti-CD3 (Cat. No. 317310) followed by fixation and permeabilization with True-Nuclear™ Transcription Factor Buffer Set (Cat. No. 424401). Then, the cells were intracellularly stained with Alexa Fluor® 594 Anti-T-Bet (clone 4B10) (left panel) or Alexa Fluor® 594 Mouse IgG1, κ isotype control (Cat. No. 400174) (right panel).
  • A_4B10_A594_T-bet_Antibody_ICFC_3_032724
    PBMCs were surface stained with Alexa Fluor® 488 anti-CD3 (Cat. No. 317310) followed by fixation and permeabilization with True-Nuclear™ Transcription Factor Buffer Set (Cat. No. 424401). Then, the cells were intracellularly stained with Alexa Fluor® 594 Anti-T-Bet (clone 4B10) (left panel) or Alexa Fluor® 594 Mouse IgG1, κ isotype control (Cat. No. 400174) (right panel).
  • B_4B10_A594_T-bet_Antibody_ICC_2_0020618
    NK-92 cells were fixed with 4% paraformaldehyde (PFA) for 15 minutes, permeabilized with 0.5% Triton X-100 for 3 minutes, and blocked with 5% FBS for 60 minutes. Then the cells were intracellularly stained with 1:400 (1.25 µg/ml), 1: 200 (2.5 µg/ml) and 1:100 diluted (5 µg/ml) Alexa Fluor® 594 anti-T-bet antibody (red) or 1:100 diluted (5 µg/ml) Alexa Fluor® 594 Mouse IgG1, κ isotype ctrl antibody overnight at 4°C. Nuclei were counterstained with DAPI (blue, Cat. No. 422801). The image was captured with a 60X objective using KEYENCE BZ-X700 fluorescence microscope.
  • C_4B10_A594_T-bet_Antibody_ICC_1_0020618
    NK-92 cells were fixed with 4% paraformaldehyde (PFA) for 15 minutes, permeabilized with 0.5% Triton X-100 for 3 minutes, and blocked with 5% FBS for 60 minutes. Then the cells were intracellularly stained with 1:200 diluted (2.5 µg/ml) Alexa Fluor® 594 anti-T-bet antibody (red). Nuclei were counterstained with DAPI (blue, Cat. No. 422801). The image was captured with a 60X objective. For negative control and titration data, refer to the supplemental data.
Compare all formats See Alexa Fluor® 594 spectral data
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644833 25 µg 128€
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644834 100 µg 296€
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Description

T-bet, also known as T-box transcription factor T-bet, is considered to be a "master regulator" of Th1 lymphoid development controlling the production of the cytokine IFN-γ. T-bet is widely expressed in hematopoietic cells including stem cells, NK cells, B cells, and T cells. T-bet is critical for the control of microbial pathogens, and knockout animals show multiple physiologic and inflammatory features characteristic of asthma. T-bet expression is optimally observed after IL-12 stimulation and can be suppressed by addition of the Th2 cytokine IL-4 or neutralization of IL-12.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Human, Mouse
Antibody Type
Monoclonal
Host Species
Mouse
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 594 under optimal conditions.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

ICFC - Quality tested
ICC - Verified
Recommended Usage

Each lot of this antibody is quality control tested by intracellular flow cytometry using our True-Nuclear™ Transcription Factor Staining Protocol. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per million cells in 100 µL volume. For immunocytochemistry, a concentration range of 2.0 - 5.0 μg/ml (1:100-1:250 dilution) is recommended. It is recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor® 594 has an excitation maximum of 590 nm, and a maximum emission of 617 nm.


Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

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Application Notes

Additional reported applications (for the relevant formats) include: immunoprecipitation2 and immunofluorescence microscopy3.

NOTE: For flow cytometric staining with this clone, True-Nuclear™ Transcription Factor Buffer Set (Cat. No. 424401) offers improved staining and is highly recommended over the Foxp3 Fix/Perm Buffer Set (Cat. No. 421403) and the Nuclear Factor Fixation and Permeabilization Buffer Set (Cat. No. 422601).

Application References

(PubMed link indicates BioLegend citation)
  1. Szabo SJ, et al. 2000. Cell 100:655. (ICFC, WB)
  2. Hwang ES, et al. 2005. J. Exp. Med. 202:1289. (ICFC, WB, IP)
  3. Neurath MF, et al. 2002. J. Exp. Med. 195:1129. (IF)
  4. Hsieh CY, et al. 2012. J Pharmacol Exp. 343:125. PubMed.
Product Citations
  1. Silva-Cayetano A, et al. 2020. Med. 2(3):243-262.e8. PubMed
RRID
AB_2728473 (BioLegend Cat. No. 644833)
AB_2728473 (BioLegend Cat. No. 644834)

Antigen Details

Structure
T-box transcription factor, approximately 58 kD.
Distribution

Nuclear; expressed in T cells, hematopoietic stem cells, NK cells, B cells, lung, spleen.

Function
Th1-specific T-box transcription factor controlling expression of the hallmark Th1 cytokine, interferon gamma (IFN-γ). T-bet expression is critical for the control of microbial pathogens.
Cell Type
B cells, Hematopoietic stem and progenitors, NK cells, T cells, Tregs
Biology Area
Cell Biology, Immunology, Transcription Factors
Molecular Family
Nuclear Markers
Antigen References

1. Szabo SJ, et al. 2000. Cell 100:655.
2. Szabo SJ, et al. 2002. Science 295:338.
3. Finotto S, et al. 2002. Science 295:336.
4. Mullen AC, et al. 2001. Science 292:1907.

Gene ID
30009 View all products for this Gene ID
UniProt
View information about T-bet on UniProt.org
Go To Top Version: 2    Revision Date: 02.09.2018

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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