- Clone
- Basophil/2D7 (See other available formats)
- Regulatory Status
- RUO
- Isotype
- Mouse IgG1, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
Cat # | Size | Price | Quantity Check Availability | Save | ||
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346204 | 100 tests | 278€ |
The Basophil/2D7 mAb specifically recognizes the human basophil population. The antigen that this antibody binds is present in cell granules but its identity is still unknown.
Product DetailsProduct Details
- Verified Reactivity
- Human
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- Lysates of purified basophils
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
- Preparation
- The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 647 under optimal conditions.
- Concentration
- Lot-specific (to obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.)
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
ICFC - Quality tested
- Recommended Usage
-
Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.
* Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633nm / 635nm.
Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.
View full statement regarding label licenses - Excitation Laser
-
Red Laser (633 nm)
- Application Notes
-
Additional reported applications for the relevant formats include: immunofluorescence staining7, and immunohistochemistry of acetone-fixed frozen sections1 and formalin-fixed paraffin-embedded sections1-6.
-
Application References
(PubMed link indicates BioLegend citation) -
- Kepley CL, et al. 1995. J. Immunol. 154:6548. (IHC)
- Irani AM, et al. 1998. J. Allergy Clin. Immunol. 1998 101:354. (IHC)
- Agis H, et al. 2006. J. Clin. Pathol. 59:396. (IHC)
- Plager DA, et al. 2006. J. Allergy Clin. Immunol. 117:626. (IHC)
- Sperr WR, et al. 2001. Blood 98:2200. (IHC)
- Aichberger KJ, et al. 2006. Blood 108:3538. (IHC)
- Kepley CL, et al. 1998. J. Allergy Clin. Immun. 102:304. (IF)
- RRID
-
AB_1967142 (BioLegend Cat. No. 346204)
Antigen Details
- Distribution
-
Basophils.
- Cell Type
- Basophils
- Biology Area
- Immunology, Innate Immunity
- Gene ID
- NA
- UniProt
- View information about Basophil on UniProt.org
Other Formats
View All Basophil Reagents Request Custom ConjugationDescription | Clone | Applications |
---|---|---|
Purified anti-human Basophils (2D7) | Basophil/2D7 | ICFC,ICC,IHC-P,IHC-F |
Alexa Fluor® 647 anti-human Basophils (2D7) | Basophil/2D7 | ICFC |
PE anti-human Basophils (2D7) | Basophil/2D7 | ICFC |
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Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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Purified anti-human Basophils (2D7)
Human peripheral blood mononuclear cells surface stained wit... -
Alexa Fluor® 647 anti-human Basophils (2D7)
Human peripheral blood mononuclear cells surface stained wit... -
PE anti-human Basophils (2D7)
Human peripheral blood mononuclear cells were stained with C...
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