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Alexa Fluor® 647 anti-human CD107b (LAMP-2) Antibody

Pricing & Availability
Clone
H4B4 (See other available formats)
Regulatory Status
RUO
Workshop
HCDM listed
Other Names
LAMPb, LGP110
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
H4B4_A647_CD107b_Antibody_111317
Human acute myeloid leukemia cell line KG1a was fixed, permeabilized, and stained with CD107b (clone H4B4) Alexa Fluor® 647 (filled histogram) or mouse IgG1, κ Alexa Fluor® 647 isotype control (open histogram).
  • H4B4_A647_CD107b_Antibody_111317
    Human acute myeloid leukemia cell line KG1a was fixed, permeabilized, and stained with CD107b (clone H4B4) Alexa Fluor® 647 (filled histogram) or mouse IgG1, κ Alexa Fluor® 647 isotype control (open histogram).
  • H4B4_A647_CD107b_Antibody_IHC_111317
    Human paraffin-embedded prostate tissue slice were prepared with a standard protocol of deparaffinization and rehydration. Antigen retrieval was done with Citrate Buffer 1X (1.0M, pH 7.4) at 95°C for 40 minutes. Tissue was washed with PBS/0.05% Tween 20 twice for five minutes and blocked with 5% FBS and 0.2% gelatin for 30 minutes. Then, the tissue was stained with 10 µg/mL of Alexa Fluor® 594 anti-human CD44 (clone IM7, green) and Alexa Fluor® 647 anti-human CD107b antibody (clone H4B4, red) overnight at 4°C. The image was scanned with a 10X objective and stitched with MetaMorph® software.
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354311 25 tests 90€
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354312 100 tests 235€
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Description

CD107b, also known as LAMP-2, is a 105 kD, highly gylcosylated, type I transmembrane protein. CD107b is expressed in lysosomal/endosomal membranes in nearly all cells, and on the surface of activated platelets, activated lymphocytes and some tumor cell lines.  LAMP-2 is known to have roles in cell adhesion and cellular homeostasis, including autophagocytosis and antigen presentation.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Adult human adherent spleen cells
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 647 under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

ICFC - Quality tested
IHC-P - Verified 
Recommended Usage

Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood. For immunohistochemistry, a concentration of 10 µg/ml is suggested. It is recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633 nm / 635 nm.


Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

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Excitation Laser
Red Laser (633 nm)
Application Notes

Additional reported applications (for the relevant formats) include: immunohistochemical staining of frozen glomeruli2 and immunofluorescent staining of  neutrophils2,3.

This antibody is specific to human LAMP-2. Positive control: Hela cells; LAMP-2 molecular weight appears to be at ~110 kDa on the gel due to high glycosylation.

Application References

(PubMed link indicates BioLegend citation)
  1. Chen J, et al. 1985. J. Biol. Chem. 101:85.
  2. Kain R, et al. 2008. Nat. Med. 14:1088. (IF, IHC)
  3. Roark EA, et al. 2008. PLoS ONE 3:e3538. (IF)
Product Citations
  1. Chin MY, et al. 2021. ACS Sens. 6:2168. PubMed
RRID
AB_2721405 (BioLegend Cat. No. 354311)
AB_2721405 (BioLegend Cat. No. 354312)

Antigen Details

Structure
LAMP-2 is a 410 amino acid protein with a molecular mass of 45 kD.
Distribution

Lysosomal/endosomal membranes in nearly all cells; surface of activated platelets, activated lymphocytes and some tumor cell lines

Function
Adhesion, cellular homeostatis, including autophagocytosis, antigen presentation
Cell Type
B cells, Lymphocytes, Platelets
Biology Area
Cell Adhesion, Cell Biology, Cell Motility/Cytoskeleton/Structure, Immunology, Innate Immunity, Neurodegeneration, Neuroscience, Protein Trafficking and Clearance
Molecular Family
Adhesion Molecules, CD Molecules
Antigen References

1. Chen J, et al. 1985. J. Biol. Chem. 101:85.
2. Kain R, et al. 2008. Nat. Med. 14:1088.
3. Roark EA, et al. 2008. PLoS ONE 3:e3538.

Gene ID
3920 View all products for this Gene ID
UniProt
View information about CD107b on UniProt.org
Go To Top Version: 0    Revision Date: 11.14.2017

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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