Alexa Fluor 647 anti-human IL-12/IL-23 p40 Antibody anti-IL-12/IL-23 p40

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Clone: C11.5 (See other available formats)
Regulatory Status: RUO
Other Names: Interleukin-12 p40, Interleukin-23 p40, Cytotoxic lymphocyte maturation factor (CLMF), Natural killer cell stimulatory factor (NKSF), CTL maturation factor (TcMF), T-cell stimulating factor (TSF)
Isotype: Mouse IgG1, κ
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IFN-γ-primed and then LPS-stimulated (overnight) human peripheral blood monocytes surface stained with CD14 (M5E2) FITC and intracellularly stained with C11.5 Alexa Fluor® 647.

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501818

100 tests

212€

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Description

The C11.5 antibody reacts with human IL-12 p40 subunit of the IL-12 p70 heterodimer and IL-23 p40 subunit of the IL-23 p19/p40, as well as p40 monomer and homodimer. The C11.5 antibody can neutralize the bioactivity of natural or recombinant IL-12 p70.

Product Details

Technical data sheet

Product Details

Verified Reactivity: Human
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: CHO-expressed, recombinant human IL-12 p70 heterod
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation: The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 647 under optimal conditions.
Concentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: ICFC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per 10⁶ cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633nm / 635nm.

Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

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Excitation Laser: Red Laser (633 nm)
Application Notes: Flow Cytometry^3,5: The fluorochrome-labeled C11.5 antibody is useful for intracellular immunofluorescent staining and flow cytometric analysis to identify IL-12 p40 (monomeric, heterodimeric, homodimeric) -producing cells within mixed cell populations. For intracellular cytokine staining protocol, please visit www.biolegend.com and click on the support section.
Neutralization⁶: The LEAF™ purified antibody (Endotoxin <0.1 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 501813).
Additional reported applications (for the relevant formats) include: Western blotting, ELISA Capture^1,2, ICFC Blocking, immunohistochemical staining⁴ of paraformaldehyde-fixed, saponin-treated tissue frozen sections, and immunocytochemistry.
Note: For testing human IL-12/IL-23 p40 (monomer, dimer) in serum or plasma, BioLegend's ELISA Max™ Sets (Cat. No. 430701 to 430706) are specially developed and recommended. For testing human IL-12 p70 in serum or plasma, BioLegend's ELISA Max™ Sets (Cat. No. 431701 to 431706) are specially developed and recommended.
RRID: AB_2124518 (BioLegend Cat. No. 501818)

Antigen Details

Structure: Cytokine; monomer, heterodimer (p40:p35 or p40:p19) or homodimer (p40:p40)
Bioactivity: IL-12 p70 (p40:p35) induces IFN-?, TNF-a production in T and NK cells; costimulation of PBL proliferation; proliferation/differentiation of TH1 T lymphocytes. IL-23 (p40:p19) induces proliferation and production of IFN-? by human memory T cells, but not n
Cell Sources: Dendritic cells, monocytes/macrophages, B cells, T cells
Cell Targets: T cells, NK cells, PBL
Receptors: IL-12Rß1 binds p40; dimeric with IL-12Rß2 binds p35
Biology Area: Immunology, Innate Immunity
Molecular Family: Cytokines/Chemokines
Antigen References: 1. Fitzgerald K, et al. Eds. 2001. The Cytokine FactsBook. Academic Press San Diego.
2. Quesniaux V. 1992. Research Immunol. 143:385.
3. Trinchieri G, et al. 1992 Prog. Growth Factor Res. 4:355.
4. Trinchieri G, et al. 1993 Immunol. Today. 14:335.
5. Oppmann B, et al. 2000 Immunity. 13:715.
6. Aggarwal S, et al. 2003 J. Biol. Chem. 278:1910.
7. Parham C, et al. 2002 J. Immunol. 168:5699.
8. Belladonna ML, et al. 2002 J. Immunol. 168:5448.
9. Lankford CS, et al. 2003 J. Leukoc. Biol. 73:49.
Regulation: Downregulated by IL-10; homodimeric p40 antagonistic to functional p70 heterodimer; p40 monomer has no function; p40 subunit in common with IL-23
Gene ID: 3593 View all products for this Gene ID
UniProt: View information about IL-12/IL-23 p40 on UniProt.org

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Other Formats

View All IL-12/IL-23 p40 Reagents Request Custom Conjugation

Description	Clone	Applications
PE anti-human IL-12/IL-23 p40 (monomer, dimer, heterodimer)	C11.5	ICFC
APC anti-human IL-12/IL-23 p40 (monomer, dimer, heterodimer)	C11.5	ICFC
FITC anti-human IL-12/IL-23 p40 (monomer, dimer, heterodimer)	C11.5	ICFC
Purified anti-human IL-12/IL-23 p40 (monomer, dimer, heterodimer)	C11.5	ICFC,IHC-F,ICC,Block,Neut,WB
Alexa Fluor® 488 anti-human IL-12/IL-23 p40	C11.5	ICFC
Alexa Fluor® 647 anti-human IL-12/IL-23 p40	C11.5	ICFC
Pacific Blue™ anti-human IL-12/IL-23 p40	C11.5	ICFC
PerCP/Cyanine5.5 anti-human IL-12/IL-23 p40 (monomer, dimer, heterodimer)	C11.5	ICFC
Ultra-LEAF™ Purified anti-human IL-12/IL-23 p40	C11.5	ICFC,IHC-F,ICC,Block,Neut,WB
TotalSeq™-B1150 anti-human IL-12/IL-23 p40	C11.5	ICPG

Certificate of Analysis

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Go To Top Version: 4 Revision Date: 09.20.2023

For Research Use Only. Not for diagnostic or therapeutic use.

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