Loading...

Alexa Fluor® 647 anti-human IL-12/IL-23 p40 Antibody

Pricing & Availability
Clone
C11.5 (See other available formats)
Regulatory Status
RUO
Other Names
Interleukin-12 p40, Interleukin-23 p40, Cytotoxic lymphocyte maturation factor (CLMF), Natural killer cell stimulatory factor (NKSF), CTL maturation factor (TcMF), T-cell stimulating factor (TSF)
Isotype
Mouse IgG1, κ
Ave. Rating
Submit a Review
Product Citations
publications
C11dot5_A647_070609.jpg
IFN-γ-primed and then LPS-stimulated (overnight) human peripheral blood monocytes surface stained with CD14 (M5E2) FITC and intracellularly stained with C11.5 Alexa Fluor® 647.
  • C11dot5_A647_070609.jpg
    IFN-γ-primed and then LPS-stimulated (overnight) human peripheral blood monocytes surface stained with CD14 (M5E2) FITC and intracellularly stained with C11.5 Alexa Fluor® 647.
Compare all formats See Alexa Fluor® 647 spectral data
Cat # Size Price Quantity Check Availability Save
501818 100 tests 212€
Check Availability


Need larger quantities of this item?
Request Bulk Quote
Description

The C11.5 antibody reacts with human IL-12 p40 subunit of the IL-12 p70 heterodimer and IL-23 p40 subunit of the IL-23 p19/p40, as well as p40 monomer and homodimer. The C11.5 antibody can neutralize the bioactivity of natural or recombinant IL-12 p70.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
CHO-expressed, recombinant human IL-12 p70 heterod
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 647 under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

ICFC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633nm / 635nm.


Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

View full statement regarding label licenses
Excitation Laser
Red Laser (633 nm)
Application Notes

Flow Cytometry3,5: The fluorochrome-labeled C11.5 antibody is useful for intracellular immunofluorescent staining and flow cytometric analysis to identify IL-12 p40 (monomeric, heterodimeric, homodimeric) -producing cells within mixed cell populations. For intracellular cytokine staining protocol, please visit www.biolegend.com and click on the support section.
Neutralization6: The LEAF™ purified antibody (Endotoxin <0.1 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 501813).
Additional reported applications (for the relevant formats) include: Western blotting, ELISA Capture1,2, ICFC Blocking, immunohistochemical staining4 of paraformaldehyde-fixed, saponin-treated tissue frozen sections, and immunocytochemistry.
Note: For testing human IL-12/IL-23 p40 (monomer, dimer) in serum or plasma, BioLegend's ELISA Max™ Sets (Cat. No. 430701 to 430706) are specially developed and recommended. For testing human IL-12 p70 in serum or plasma, BioLegend's ELISA Max™ Sets (Cat. No. 431701 to 431706) are specially developed and recommended.

Application References

(PubMed link indicates BioLegend citation)
  1. D'Andrea A, et al. 1992. J. Exp. Med. 176:1387.
  2. D'Andrea A, et al. 1993. J. Exp. Med. 178:1041.
  3. Prussin C, et al. 1995. J. Immunol. Meth. 188:117.
  4. Leifeld L, et al. 2002. Hepatology 36:1001.
  5. Pekkari K, et al. 2001. Blood 97:3184.
  6. Bolovan-Fritts CA, et al. 2004. J. Virol. 78:13173.
Product Citations
  1. Etxeberria I, et al. 2020. Cancer Cell. 36(6):613-629. PubMed
  2. Nishibayashi R, et al. 2015. PLoS One. 10:129806. PubMed
RRID
AB_2124518 (BioLegend Cat. No. 501818)

Antigen Details

Structure
Cytokine; monomer, heterodimer (p40:p35 or p40:p19) or homodimer (p40:p40)
Bioactivity
IL-12 p70 (p40:p35) induces IFN-?, TNF-a production in T and NK cells; costimulation of PBL proliferation; proliferation/differentiation of TH1 T lymphocytes. IL-23 (p40:p19) induces proliferation and production of IFN-? by human memory T cells, but not n
Cell Sources
Dendritic cells, monocytes/macrophages, B cells, T cells
Cell Targets
T cells, NK cells, PBL
Receptors
IL-12Rß1 binds p40; dimeric with IL-12Rß2 binds p35
Biology Area
Immunology, Innate Immunity
Molecular Family
Cytokines/Chemokines
Antigen References

1. Fitzgerald K, et al. Eds. 2001. The Cytokine FactsBook. Academic Press San Diego.
2. Quesniaux V. 1992. Research Immunol. 143:385.
3. Trinchieri G, et al. 1992 Prog. Growth Factor Res. 4:355.
4. Trinchieri G, et al. 1993 Immunol. Today. 14:335.
5. Oppmann B, et al. 2000 Immunity. 13:715.
6. Aggarwal S, et al. 2003 J. Biol. Chem. 278:1910.
7. Parham C, et al. 2002 J. Immunol. 168:5699.
8. Belladonna ML, et al. 2002 J. Immunol. 168:5448.
9. Lankford CS, et al. 2003 J. Leukoc. Biol. 73:49.

Regulation
Downregulated by IL-10; homodimeric p40 antagonistic to functional p70 heterodimer; p40 monomer has no function; p40 subunit in common with IL-23
Gene ID
3593 View all products for this Gene ID
UniProt
View information about IL-12/IL-23 p40 on UniProt.org
Go To Top Version: 4    Revision Date: 09.20.2023

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

ProductsHere
Login / Register
Remember me
Forgot your password? Reset password?
Create an Account