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- Clone
- LpMab-21 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- T1a, T1a2, gp36, gp38, gp40, Aggrus, PDPN, D2-40
- Isotype
- Mouse IgG2a, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
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Human LN319 cell line was stained with anti-human podoplanin (clone LpMab-21) Alexa Fluor® 647 (filled histogram) or mouse IgG2a, κ isotype control (clone MOPC-173) Alexa Fluor® 647 (open histogram). -
Human paraffin-embedded normal breast tissue slices were prepared with a standard protocol of deparaffinization and rehydration. Antigen retrieval was done with Citrate buffer pH 6.0 1X at 95°C for 40 minutes. Tissue was washed with PBS/0.05% Tween 20 twice for five minutes, permealized with 0.5% Triton-X 100 and blocked with 5% FBS and 0.2% gelatin for 30 minutes. Then, the tissue was stained with 10 µg/mL of Alexa Fluor® 647 anti-human podoplanin (clone Lpmab-21, red) and Alexa Fluor® 594 anti-vimentin (clone O91D3, green) at 4°C overnight. Nuclei were counterstained with DAPI (Blue). The image was scanned with a 10X objective and stitched with Metamorph® software.
| Cat # | Size | Price | Quantity Check Availability | Save | ||
|---|---|---|---|---|---|---|
| 395003 | 25 tests | 128€ | ||||
| 395004 | 100 tests | 287€ | ||||
Podoplanin is a 40-43 kD type-I transmembrane sialomucin-type glycoprotein, also known as T1a, gp36, gp38, gp40, and Aggrus. Originally detected on the surface of podocytes, futher characterization showed podoplanin has a broad tissue distribution, including mesothelial cells, epithelial cells, follicular dendritic cells, and a variety of tumor cells. It has been reported that podoplanin is the ligand of CLEC2 and is involved in lymphatic vessel formation, platelet aggregation, and tumor metastasis. Podoplanin may serve as a useful marker for tumor diagnosis and prognosis.
Product DetailsProduct Details
- Verified Reactivity
- Human
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- Recombinant human podoplanin
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
- Preparation
- The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 647 under optimal conditions.
- Concentration
- Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
FC - Quality tested
IHC-P - Verified - Recommended Usage
-
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood. For immunohistochemistry on formalin-fixed paraffin-embedded tissue sections, a concentration range of 5.0 - 10 µg/ml is suggested. It is recommended that the reagent be titrated for optimal performance for each application.
* Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633 nm / 635 nm.
Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.
View full statement regarding label licenses - Excitation Laser
-
Red Laser (633 nm)
-
Application References
(PubMed link indicates BioLegend citation) -
- Kaneko MK, et al. 2017. Cancer Med. 6(2):382-396 (IHC-P, FC)
- Product Citations
-
- RRID
-
AB_2783309 (BioLegend Cat. No. 395003)
AB_2783309 (BioLegend Cat. No. 395004)
Antigen Details
- Structure
- 40-43 kD type-I transmembrane sialomucin-type glycoprotein
- Distribution
-
Broad tissue distribution, including mesothelial cells, epithelial cells, follicular dendritic cells, and many tumor cells
- Function
- Lymphatic vessel formation, tumor metastasis, platelet aggregation
- Ligand/Receptor
- CLEC2
- Biology Area
- Cancer Biomarkers, Cell Biology, Neuroinflammation, Neuroscience, Neuroscience Cell Markers
- Antigen References
-
- Raica M, et al. 2008. Anticancer Res. 28:2997.
- Xie Q, et al. 2008. Int. J. Clin. Exp. Pathol. 1:276.
- Ogasawara S, et al. 2008. Hybridoma. 27:259.
- Kato Y, et al. 2003. J. Biol. Chem. 278:51599.
- Gene ID
- 10630 View all products for this Gene ID
- UniProt
- View information about Podoplanin on UniProt.org
Related Pages & Pathways
Pathways
Other Formats
View All Podoplanin Reagents Request Custom Conjugation| Description | Clone | Applications |
|---|---|---|
| Purified anti-human Podoplanin | LpMab-21 | FC,IHC-P |
| Alexa Fluor® 647 anti-human Podoplanin | LpMab-21 | FC,IHC-P |
| Alexa Fluor® 594 anti-human Podoplanin | LpMab-21 | IHC-P |
Customers Also Purchased
Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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Purified anti-human Podoplanin
Human glioblastoma cell line LN319 was stained with podoplan... 
Human paraffin-embedded breast cancer tissue slices were pre... 
Human paraffin-embedded breast cancer tissue slices were pre... -
Alexa Fluor® 647 anti-human Podoplanin
Human LN319 cell line was stained with anti-human podoplanin... 
Human paraffin-embedded normal breast tissue slices were pre... -
Alexa Fluor® 594 anti-human Podoplanin
Human paraffin-embedded breast cancer tissue slices were pre... 
Human paraffin-embedded normal breast tissue slices were pre...
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