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Alexa Fluor® 647 anti-mouse CD279 (PD-1) Antibody

Pricing & Availability
Clone
RMP1-30 (See other available formats)
Regulatory Status
RUO
Other Names
Programmed Death-1, CD279, PDCD1
Isotype
Rat IgG2b, κ
Ave. Rating
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Product Citations
publications
RMP1-30_A647_PD-1_Antibody_FC_1_112515
Con A (three-days) activated C57BL/6 splenocytes were stained with CD3 PE and CD279 (clone RMP1-30) Alexa Fluor® 647 (top) or rat IgG2b, κ Alexa Fluor® 647 isotype control (bottom).
  • RMP1-30_A647_PD-1_Antibody_FC_1_112515
    Con A (three-days) activated C57BL/6 splenocytes were stained with CD3 PE and CD279 (clone RMP1-30) Alexa Fluor® 647 (top) or rat IgG2b, κ Alexa Fluor® 647 isotype control (bottom).
  • RMP1-30_A647_PD-1_Antibody_FC_2_112515
Compare all formats See Alexa Fluor® 647 spectral data
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109117 25 µg 104€
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109118 100 µg 235€
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Description

CD279 is a 50-55 kD immunoglobulin superfamily member also known as programmed death-1 (PD-1). PD-1 is expressed on a subset of CD4-CD8- thymocytes and on activated T and B cells. PD-1 is thought to be involved in lymphocyte clonal selection and peripheral tolerance. The PD-1 ligands, PD-L1 (also known as B7-H1) and PD-L2 (B7-DC), are members of the B7 immunoglobulin superfamily.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Mouse PD-1 transfected BHK cells
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 647 under optimal conditions.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633 nm / 635 nm.


Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

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Excitation Laser
Red Laser (633 nm)
Application Notes

The RMP1-30 antibody does not block the binding of PD-1 to B7-H1 and B7-DC1.

Application References

(PubMed link indicates BioLegend citation)
  1. Matsumoto K, et al. 2004. J. Immunol. 172:2530.
  2. Raimondi G, et al. 2006. J. Immunol. 176:2808. (FC) PubMed
  3. King IL, et al. 2009. J. Exp Med 206:1001. (FC) PubMed
Product Citations
  1. Grégoire C, et al. 2022. Immunity. 55:1216. PubMed
  2. Melgrati S, et al. 2023. Front Immunol. 1242531:14. PubMed
  3. Turner JA, et al. 2020. Immunity. 53:1202. PubMed
  4. Wong HS, et al. 2021. Cell. . PubMed
  5. Harb H, et al. 2021. Immunity. 54(6):1186-1199.e7. PubMed
  6. Xu JL, et al. 2022. Front Pharmacol. 13:911663. PubMed
  7. Song W, et al. 2022. Immunity. 55:290. PubMed
RRID
AB_2566550 (BioLegend Cat. No. 109117)
AB_2566550 (BioLegend Cat. No. 109118)

Antigen Details

Structure
Ig superfamily, 50-55 kD
Distribution

Subset of double negative thymocytes, activated T and B cells

Function
Lymphocyte clonal selection, peripheral tolerance
Ligand/Receptor
PD-L1 (B7-H1), PD-L2
Cell Type
B cells, T cells, Thymocytes, Tregs
Biology Area
Apoptosis/Tumor Suppressors/Cell Death, Cancer Biomarkers, Cell Biology, Immunology, Inhibitory Molecules
Molecular Family
CD Molecules, Immune Checkpoint Receptors
Antigen References

1. Barclay A, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Agata Y, et al. 1996. Int. Immunol. 8:765.
3. Nishimura H, et al. 2001. Science 291:319.
4. Ishida Y, et al. 1992. EMBO J. 11:3887.

Gene ID
18566 View all products for this Gene ID
UniProt
View information about CD279 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 11.30.2015

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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