Alexa Fluor 647 anti-mouse IL-9 Antibody anti-IL-9

Pricing & Availability

Clone: RM9A4 (See other available formats)
Regulatory Status: RUO
Other Names: Interleukin-9, P40, Mast cell growth factor (MCGF), Megakaryoblast growth factor, T cell growth factor-3 (TCGF-3)
Isotype: Rat IgG1, κ
Ave. Rating: Submit a Review
Product Citations: publications

CD4+ T cells from C57BL/6 mouse were cultured with anti-mouse CD3 and CD28 in the presence of recombinant IL-4 and TGF-beta for three days. Intracellular staining was performed following 5 hour restimulation with PdBU and ionomycin (in the presence of brefeldin A)

Compare all formats See Alexa Fluor® 647 spectral data

Cat #

Size

Price

Quantity Check Availability

Save

514107

25 µg

151€

Check Availability

Need larger quantities of this item?
Request Bulk Quote

Description

IL-9 is a potent, T cell-derived, T cell growth factor which can also enhance mast cell activity and IL-3- or IL-4- dependent proliferation of bone marrow-derived mast cells. IL-9 synergizes with erythropoietin to promote erythroid colony formation. IL-9 induces high affinity IgE receptor expression and granzyme A and B in murine T helper clones.

Product Details

Technical data sheet

Product Details

Verified Reactivity: Mouse
Antibody Type: Monoclonal
Host Species: Rat
Immunogen: Recombinant full length mouse IL-9
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation: The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 647 under optimal conditions.
Concentration: 0.5 mg/ml
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: ICFC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.06 µg per 10⁶ cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633nm / 635nm.

Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

View full statement regarding label licenses
Excitation Laser: Red Laser (633 nm)
Application Notes: See the protocol for Th9 polarization of mouse splenocytes.
RRID: AB_2126637 (BioLegend Cat. No. 514107)

Antigen Details

Structure: Cytokine; 30-40 kD (Mammalian)
Bioactivity: Potentiates production of IgG, IgM, and IgE by IL-4-induced B lymphocytes; regulates granzyme family proteases; enhances proliferation of bone marrow mast cells with IL-3; production of IL-6 by mast cells
Cell Sources: IL-2 activated T_H2 lymphocytes
Cell Targets: T lymphocytes, erythroid precursors
Receptors: IL-9R
Biology Area: Immunology
Molecular Family: Cytokines/Chemokines
Antigen References: 1. Fitzgerald K, et al. Eds. 2001. The Cytokine FactsBook. Academic Press San Diego.
2. Quesniaux V, et al. 1992. Research Immunology 143:385.
3. Renauld J, et al. 1993. Adv. Immunol. 54:79.
4. Yang Y, et al. 1992. Leuk. Lymphoma 8:441.
Regulation: Upregulated by IL-1 after TCR activation, TNF-α; downregulated by antibodies to IL-2
Gene ID: 16198 View all products for this Gene ID
UniProt: View information about IL-9 on UniProt.org

Documentation

Reviews

Review This Product

Related Protocols

Related Products

Description	Clone	Applications

Related Pages & Pathways

Pathways

Related FAQs

There are no FAQs for this product.

Other Formats

View All IL-9 Reagents Request Custom Conjugation

Description	Clone	Applications
PE anti-mouse IL-9	RM9A4	ICFC
Purified anti-mouse IL-9	RM9A4	ELISA
APC anti-mouse IL-9	RM9A4	ICFC
Alexa Fluor® 647 anti-mouse IL-9	RM9A4	ICFC
Brilliant Violet 421™ anti-mouse IL-9	RM9A4	ICFC
PerCP/Cyanine5.5 anti-mouse IL-9	RM9A4	ICFC

Certificate of Analysis

Customers Also Purchased

Brilliant Violet 711™ anti-mouse CD8a

Clone: 53-6.7

Brilliant Violet 711™ anti-mouse Ly-6G

Clone: 1A8

Brilliant Violet 650™ anti-mouse IL-17A

Clone: TC11-18H10.1

Brilliant Violet 711™ anti-human CD279 (PD-1)

Clone: EH12.2H7

Go To Top Version: 2 Revision Date: 06.27.2014

For Research Use Only. Not for diagnostic or therapeutic use.

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.